Glucose tolerance tests were performed after an overnight fasting. Blood glucose was measured before and 15, 30, 60 and 120 min after an i.p. glucose injection (1 g kg−1) with a Freestyle Glucose Monitoring System (Abbott Laboratories). Insulin tolerance testing was performed after a 6-h fast. Blood glucose was measured before and 15, 30, 60 and 120 min after an i.p. insulin injection (0.75 U kg−1; Novolin R, Novo Nordisk Inc.).
Freestyle glucose monitoring system
Manufactured by Abbott
The FreeStyle Glucose Monitoring System is a handheld device designed to measure and monitor blood glucose levels. It is a compact and portable tool for individuals to track their glucose readings.
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5 protocols using freestyle glucose monitoring system
1
Glucose and Insulin Tolerance Tests
2
Glucose and Insulin Tolerance Assays
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Glucose tolerance tests (GTTs) and Insulin tolerance tests (ITTs) were performed following our established protocol38 (link). Briefly, GTTs were performed after an overnight (for CC and HFD-fed mice or db/db mice) fasting. Blood glucose was measured before and 15, 30, 60, 120 min after an i.p. glucose injection (1 g/kg) with a Freestyle Glucose Monitoring System (Abbott Laboratories). ITTs were performed after 4 h fasting. Blood glucose was measured before and indicated time periods after an i.p. insulin injection.
3
Pyruvate Tolerance Test in Mice
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To determine pyruvate tolerance, mice were fasted for 16 hr, and Na-pyruvate was administered (1 g/kg body weight) by intraperitoneal injection. Blood glucose levels were monitored at 0, 15, 30, 60, and 120 min by a Freestyle Glucose Monitoring System (Abbott Laboratories).
4
Glucose, Insulin, and Osteocalcin Tolerance Tests
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Glucose tolerance tests (GTTs) were performed after an overnight (for CC and HFD-fed mice) or 6 h fasting (for STZ-induced diabetes mice) following the published protocol52 (link). Blood glucose was measured after an i.p. glucose injection (1 g/kg) with a Freestyle Glucose Monitoring System (Abbott Laboratories). Insulin (ITTs) and OCN tolerance testing (OTTs) were performed after 4 h fasting. Blood glucose was measured after an intravenous injection of OCN (150 μg/kg, Abcam) for OTTs, and an i.p. insulin injection at 0.5 U/kg for ITTs in CC-fed (Supplementary Fig. 4a ) and HFD-fed mice (Fig. 6f ), and 1 U/kg for HFD-fed mice (Fig. 4a ).
5
Glucose and Insulin Tolerance Tests
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Glucose tolerance tests (GTTs) and Insulin tolerance tests (ITTs) were performed following our established protocol 36 . Briefly, GTTs were performed after an overnight (for CC and HFD-fed mice or db/db mice) fasting. Blood glucose was measured before and 15, 30, 60, 120 min after an i.p. glucose injection (1 g/kg) with a Freestyle Glucose Monitoring System (Abbott Laboratories). ITTs were performed after 4 hours fasting. Blood glucose was measured before and indicated time periods after an i.p. insulin injection.
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