Hf000mc100

Manufactured by Merck Group

Sourced in Germany

The HF000MC100 is a laboratory equipment product from Merck Group. It is a piece of equipment designed for basic laboratory operations. The core function of this product is to perform measurements and analysis of samples in a controlled laboratory environment. Further details about the intended use or specific capabilities of this product are not available.

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Lab products found in correlation

6 protocols using hf000mc100

Fabrication and Characterization of Magnetic Immunosensor

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Multiwalled carbon nanotubes (MWCNTs, SN2302, purity>95%) were purchased from Nanomaterial Store (Fremont, CA, https://www.nanomaterialstore.com/), FeCl₂·4H₂O (purity>99%) was purchased from Acros Organics BVBA (Geel, Belgium, http://www.acros.com/), FeCl₃·6H₂O, N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), N-hydroxysulfosuccinimide (sulfo-NHS), 2-(4-Morpholino) ethanesulfonic acid (MES), streptavidin, sucrose, Tween 20, bovine serum albumin (BSA), Hexadecyltrimethylammonium bromide (CTAB) and phosphate buffer saline (0.01 M, pH 7.4) were purchased from Sigma-Aldrich (St. Louis, MO, http://www.sigmaaldrich.com/). Glass ﬁbers (GFCP000800), cellulose ﬁber (CFSP001700), nitrocellulose membranes (HF090MC100, HFB18004 and HFB24004) and laminated cards (HF000MC100) were purchased from Millipore (Billerica, MA, http://www.merckmillipore.com/). CA 19-9 protein, Mouse monoclonal CA 19-9 antibody 10-CA19A (Ab₁) and 10-CA19B (Ab₂) were purchased from Fitzgerald Industries International (Acton, MA, https://www.fitzgerald-fii.com/). Goat anti-mouse IgG (Ab₃) were purchased from ThermoFisher Scientific (Rockford, IL, https://www.thermofisher.com/).
All the chemicals used in this study were analytical reagent grade. Solutions were prepared with ultrapure (Z18 MΩ) water from Millipore Milli-Q water puriﬁcation system (Billerica, MA, http://www.merckmillipore.com).

Huang Y., Wen Y., Baryeh K., Takalkar S., Lund M., Zhang X, & Liu G. (2017). Lateral flow assay for carbohydrate antigen 19-9 in whole blood by using magnetized carbon nanotubes. Mikrochimica acta, 184(11), 4287-4294.

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Immunoassay Development Using Tetraspanins

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Anti-tetraspanin antibodies were purchased at Immunostep (Salamanca, Spain): anti-CD9 (clone VJ1/20), anti-CD63 (clone Tea 3/18), and anti-CD147 (clone VJ1/9). Anti-mouse IgG, bovine serum albumin (BSA), 1-ethyl-3-[3-dimethylaminopropyl]-carbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS) were provided by Sigma-Aldrich (Madrid, Spain). HEPES was purchased from Fisher Scientific.
Some materials for strips were provided by Millipore (Darmstadt, Germany): glass fibre membrane (GFCP001000) used as sample pad, nitrocellulose membrane (HF07504XSS) and backing cards (HF000MC100). Absorbent pad was purchased from Whatman (Piscataway, NJ, USA). The sample buffer used was 10 mM HEPES pH 7.4 with 0.05% Tween-20 and 1% BSA.
To prepare the detection and control lines, an IsoFlow reagent dispensing system was used. A guillotine Fellowes Gamma (Madrid, Spain) was used to cut the strips. In order to quantify the intensity of the test line using reflectance measurements, a portable strip reader ESE Quant LR3 lateral flow system (Qiagen Inc., Hilden, Germany) was used.

Moyano A., Serrano-Pertierra E., Duque J.M., Ramos V., Teruel-Barandiarán E., Fernández-Sánchez M.T., Salvador M., Martínez-García J.C., Sánchez L., García-Flórez L., Rivas M, & Blanco-López M.D. (2021). Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker Detection. Sensors (Basel, Switzerland), 21(11), 3756.

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Fabrication of Lateral Flow Assay

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FeSO₄·7H₂O (>99%), FeCl₃ (97%), NH₄OH, oleic acid (90%), myristic acid (>98%), and lauric acid (>98%) were purchased from Merk Schuchardt OHG (Hehenbrunn, Germany) and used without any further modification.
Neutravidin protein was obtained from Thermo Fischer Scientific (Waltham, MA, USA). 1-ethyl-3-[3-dimethylpropyl] carbodiimide (EDC), bovine serum albumin (BSA), biotin-conjugated bovine serum albumin (BBSA), n-hydroxysuccinimide (NHS), 2-(n-morpholino) ethanesulfonic acid (MES), and Tween20 were purchased from Sigma-Aldrich (Madrid, Spain). Glass fiber membranes (GFCP001000), used as sample pad and backing cards (HF000MC100), were purchased from Millipore (Darmstadt, Germany). Other materials used were nitrocellulose membranes (UniSart CN95, Sartorius, Spain) and absorbent pads (Whatman, Piscataway, NJ, USA).

Salvador M., Marqués-Fernández J.L., Martínez-García J.C., Fiorani D., Arosio P., Avolio M., Brero F., Balanean F., Guerrini A., Sangregorio C., Socoliuc V., Vekas L., Peddis D, & Rivas M. (2022). Double-Layer Fatty Acid Nanoparticles as a Multiplatform for Diagnostics and Therapy. Nanomaterials, 12(2), 205.

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Lateral Flow Assay Materials

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Glass fibers (GFCP000800), cellulose fiber sample pads (CFSP001700), laminated cards (HF000MC100) and nitrocellulose membranes (HFB24004) were provided by Millipore (Bedford, MA). Polyvinylpyrrolidone (PVP K30) was obtained from Fluka (www.fluka.org). Na₃PO₄ 12H₂O, HAuCl₄, trisodium citrate, sucrose, Tween 20, Triton X-100, phosphate buffer saline (PBS, pH 7.4, 0.01 mol L⁻¹ NaH₂PO₄ and Na₂HPO₄ containing 0.9% NaCl), silver nitrate (AgNO₃), hexamethylenetetramine (HTM, C₆H₁₂N₄), ascorbic acid, bovine serum albumin (BSA), human serum albumin (HSA) and casein (from bovine milk) were purchased from Sigma-Aldrich (www.sigmaaldrich.com). Rabbit IgG, Polyclonal goat anti-rabbit immunoglobulin G (IgG) antibody, mouse anti goat immunoglobulin G secondary antibody and human IgM were purchased from Thermo Scientific (www.thermofisher.com).

Yang Y., Ozsoz M, & Liu G. (2017). Gold nanocage-based lateral flow immunoassay for immunoglobulin G. Mikrochimica acta, 184(7), 2023-2029.

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Protein Immunoassay Development Protocol

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Cellulose membrane (CFSP001700), nitrocellulose membrane (HF180), glass fibber (GFCP00080000) and adhesive laminated cards (HF000MC100) were purchased from Millipore. Bovine serum albumin (BSA), sucrose, polyclonal goat anti-human IgG (I1886), Human IgG (I2511), phosphate buffer saline (PBS) tablets, sodium dodecyl sulfate (SDS), sodium tetraborate, orthoboric acid, drying pearls "orange" and Tween 20 were purchased from Sigma-Aldrich. Chicken anti-goat IgG (ab86245) was purchased from Abcam.

Quesada-González D., Sena-Torralba A., Wicaksono W.P., de la Escosura-Muñiz A., Ivandini T.A, & Merkoçi A. (2019). Iridium oxide (IV) nanoparticle-based lateral flow immunoassay. Biosensors & bioelectronics, 132.

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Lateral Flow Assay for Histamine Detection

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Mouse histamine monoclonal antibody (MBS2025715) and histamine-BSA conjugate antigen (MBS358205) were purchased from Mybiosource. Anti-mouse IgG, Bovine Serum Albumin (BSA), 1ethyl-3-[3-dimethylaminopropyl]-carbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), 2-(N-Morpholino)ethanesulfonic acid (MES) and histamine dihydrochloride were provided by Sigma-Aldrich (Spain). Recombinant protein A/G was purchased at Thermo-Scientific (Massachusetts, USA).
Gold nanoparticles of size 40 nm were purchased from BB International (UK). Disposable 0.45 µm PVDF filters were purchased from GE Healthcare Life Sciences.
Glass fibre membrane (GFCP001000) used as sample pad and backing cards (HF000MC100) were purchased from Millipore (Germany). Other materials used were nitrocellulose membranes (UniSart CN95, Sartorius, Spain) and absorbent pads (Whatman, USA). Based on previous results, the sample buffer consisted of 10 mM Phosphate-Buffer (PB) pH 7.4 with 0.5% Tween-20 and 1% BSA.
An IsoFlow reagent dispensing system (Imagene Technology, USA) was used to dispense the detection lines (dispense rate 0.100 µL/mm) and the strips were cut with a guillotine Fellowes Gamma (Spain).
A portable strip reader ESE Quant LR3 lateral flow system (Qiagen Inc., Germany) was used to quantify the intensity of the test line by reflectance measurements.

Moyano A., Salvador M., Martínez-García J.C., Socoliuc V., Vékás L., Peddis D., Alvarez M.A., Fernández M., Rivas M, & Blanco-López M.C. (2019). Magnetic immunochromatographic test for histamine detection in wine. Analytical and bioanalytical chemistry, 411(25).

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