Nicotine (cat no. N5768), α-bungarotoxin (α-BTX; a competitive irreversible antagonist of α7nAChR; cat no. T0195) and puromycin (cat no. P9620) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The MEK1/2 inhibitor U0126 (cat no. S1901) was purchased from Beyotime Biotechnology (Shanghai, China). Antibodies against ERK (cat no. 4695), phospho-p42/44 ERK (cat no. 4370), vimentin (cat no. 5741), β-actin (cat no. 4970), GAPDH (cat no. 5174) and anti-rabbit IgG, an HRP-linked antibody (cat no. 7074) used for western blotting or immunofluorescence, were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). The anti-α7nAChR antibody was purchased from Abcam (Cambridge, MA, USA; cat no. ab24644).
α bungarotoxin α btx
Manufactured by Merck Group
Sourced in United States
α-bungarotoxin (α-BTX) is a neurotoxin isolated from the venom of the banded krait snake (Bungarus multicinctus). It is a protein that selectively and reversibly binds to nicotinic acetylcholine receptors, blocking their function. α-BTX is commonly used as a research tool to study the structure and function of these receptors.
Automatically generated - may contain errors
Lab products found in correlation
Nicotine, by Merck Group (3 mentions)
Puromycin, by Merck Group (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Anti α7 nachr antibody, by Abcam (1 mentions)
Vimentin, by Cell Signaling Technology (1 mentions)
U0126, by Beyotime (1 mentions)
Y 27632, by Merck Group (1 mentions)
Gapdh sc 25778, by Santa Cruz Biotechnology (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Enhanced chemiluminescence reagent, by Cytiva (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Bcl 2, by Abcam (1 mentions)
Antibodies against gapdh, by Merck Group (1 mentions)
Cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions)
α7nachr, by Abcam (1 mentions)
Control plasmid, by Santa Cruz Biotechnology (1 mentions)
4 protocols using α bungarotoxin α btx
1
Investigating α7nAChR-mediated Signaling Pathways
2
Gastrocnemius Muscle Fiber and NMJ Analysis
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The mid-belly of the gastrocnemius muscle was trimmed for cryosectioning after post-fixation with 4% paraformaldehyde. Two sets of sections were prepared from each animal; one set was transversally sectioned for routine hematoxylin staining to count the number of muscular fibers. The second set was longitudinally sectioned for neuromuscular junction staining with α-bungarotoxin (α-BTX; 1:500; Sigma) as previously described (Anderson et al., 1984). The area of the junction was calculated. All quantifications were done in six random non-overlapping fields of every eighth section of each animal using Image-Pro Plus software.
3
Molecular Mechanisms in Cell Signaling
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Nicotine, Y27632, and α-bungarotoxin (α-Btx) were purchased from Sigma–Aldrich (St Louis, MO, USA). Antibodies against RhoGDIA (10509-1), RhoA (10749-1), RAC1 (24072-1), CDC42 (10155-1), MLC (10906-1), PAK1 (21401-1), MYPT1 (22117-1), desmin (16520-1), calponin (24855-1), and caldesmon (20887-1) were obtained from Proteintech Group Incorporation (Chicago, IL, USA). Actin (sc-10731), p-MYPT1 (sc-17432), p-PAK1 (sc-31685), p-MLC (sc-12896) and GAPDH (sc-25778) antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). An antibody against Alpha-Actin (#1184-1) was obtained from Epitomics (Burlingame, CA, USA). The Rho and Rac1/Cdc42 activation assay biochem kits were purchased from Cytoskeleton Incorporation (Denver, IL, USA). Other chemicals were of the highest grade commercially available.
4
Nicotine and α-BTX Signaling Pathways
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4NQO, nicotine and α-Bungarotoxin (α-BTX) were obtained from Sigma-Aldrich (St. Louis, MO, USA). TRIzol and Lipofectamine™ 2000 were obtained from Invitrogen Life Technologies (Grand Island, NY, USA). cDNA Reverse Transcription Kit and SYBR Green Dye reagent were obtained from Applied Biosystems (Grand Island, NY, USA). Antibodies against Prx1, α3nAChR, α7nAChR and Bcl-2 were purchased from Abcam (Cambrige, MA, USA); antibodies against p-p38, p-JUN N-terminal kinase (JNK), p-extracellular signal-regulated kinase (ERK) and all secondary antibodies were purchased from Cell signaling Technology (Beverly, MA, USA); antibodies against GAPDH were purchased from Sigma-Aldrich (St. Louis, MO, USA). Enhanced chemiluminescence reagent was purchased from Amersham Biosciences (Pittsburgh, PA, USA). Prx1 shRNA lentivirus plasmid and control shRNA lentivirus plasmid-A were purchased from Suzhou gemma gene (Suzhou, China). α3nAChR shRNA plasmid and control plasmid were purchased from Santa Cruz Biotechnology (Dallas, Texas, USA).
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