The analysis of the bonding architecture and identification of functional groups present in the samples was performed by Fourier transform infrared (FTIR) spectroscopy with a Perkin Elmer Spectrum BX II spectrometer (PerkinElmer, Inc., Waltham, MA, USA), in attenuated total reflectance (ATR) mode using a Pike-Miracle head with diamond-ZnSe crystal. The spectra were recorded in the range 4000–500 cm−1, at a resolution of 4 cm−1 and 32 scans/experiment.
Spectrum bx 2 spectrometer
Manufactured by PerkinElmer
Sourced in United States
The Spectrum BX II spectrometer is a laboratory instrument designed for spectroscopic analysis. It is capable of measuring the absorption or transmission of light across a range of wavelengths, allowing users to identify and quantify the composition of samples.
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Lab products found in correlation
4 protocols using spectrum bx 2 spectrometer
1
FTIR Spectroscopy of Sample Bonding
2
Chitosan Nanoparticles for AS1411 Aptamer Delivery
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AS1411 aptamer was purchased from TAG (Copenhagen, Denmark). N,Nʹ-Dicyclohexylcarbodiimide (DCC), chitosan nanoparticles and CaCl2 were supplied by Merck (Darmstadt, Germany). N-Hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) were obtained from Sigma-Aldrich (St Louis, MO, USA). HEK-293 and T47D cell lines were purchased from the Pasteur Institute of Iran (Tehran, Iran). Dynamic light scattering (DLS) and electrophoretic light scattering (ELS) to measure the zeta potential were performed using a Malvern Nano ZS (Malvern Instruments, Malvern, UK). Atomic force microscopy (AFM) images were captured using a NanoWizard II (JPK Instruments, Berlin, Germany). Fourier-transform infrared spectroscopy (FT-IR) experiments were conducted using a Perkin Elmer Spectrum BX-II spectrometer (Nizhni Novgorod, Russia). All other chemical reagents were of analytical grade and used without additional purification. The animal experiments were approved by the Tehran University of Medical Sciences (number IR.TUMS.PSRC.REC.1396.2340) and followed the guidelines of the university ethics committee.
3
Infrared Spectroscopy of AS1411-Chitosan Conjugates
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Using a Perkin Elmer Spectrum BX-II spectrometer, the infrared spectrum was evaluated to confirm the synthesis of the labeled AS1411–chitosan conjugates.
4
Infrared Spectroscopy for Functional Groups
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The presence of functional groups was established by Fourier transform infrared (FTIR) spectroscopy in attenuated total reflectance (ATR) mode. A Perkin Elmer Spectrum BX II spectrometer (Perkin Elmer, Waltham, MA, USA) equipped with a Pike-MIRacle ATR head with diamond-ZnSe crystal plate, having a diameter of 1.8 mm (Pike Technologies, Madison, WI, USA) was used. The spectra were acquired in the 450–3800 cm−1 spectral range. The resolution was 4 cm−1 and represented the average of 32 individual scans.
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