For scanning electron microscopy (SEM) analysis, different plant tissues were collected and placed in FAA (3.7% formaldehyde, 5% acetic acid, 50% EtOH, v/v/v) solution until use. Then the FAA was removed and the tissues were washed in an increasing gradient of ethanol (up to 100%). Fixed samples were critical point dried, mounted on a copper plate, and gold coated. Samples were viewed in a Jeol 5410 LV microscope (Jeol, Tokyo, Japan).
5410 lv microscope
Manufactured by JEOL
Sourced in Japan
The JEOL 5410 LV microscope is a scanning electron microscope (SEM) designed for high-resolution imaging of samples. It features a thermionic electron gun and provides magnification up to 300,000x. The 5410 LV is capable of examining a wide range of sample types, including solid, non-conductive, and low-vacuum specimens.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using 5410 lv microscope
1
Scanning Electron Microscopy of Plant Tissues
2
Protocols for SEM and DAPI Analysis of Pollen Grains
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For SEM analysis, pollen grains were collected and placed in FAA (3.7% formaldehyde, 5% acetic acid, 50% EtOH) solution until use. Then the FAA solution was removed and pollen grains were dehydrated in an increasing gradient of ethanol (up to 100%), critical-point-dried, mounted on a copper plate and gold-coated. Samples were viewed in a Jeol 5410 LV microscope (Tokyo, Japan). To stain pollen grains with DAPI, grains at different developmental stages were released by vortex into a DAPI solution (0.1 M sodium phosphate buffer (pH 7), 1 mM EDTA, 0.1% Triton X-100, 0.4 µg/mL DAPI), incubated for 10 min at room temperature and then viewed by Olympus IX81/FV500 laser-scanning confocal microscope (Olympus) at 361 nm maximum absorption, 461 nm maximum emission.
3
Visualizing Leaf Primordium Development
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Images analyzing the early developmental stages of the whole leaf primordium were captured using an Olympus SZX7 stereo micro-scope (http://www.olympus.com/) equipped with a Nikon DXM1200 camera and ACTA software, or a Nikon SMZ1270 stereo micro-scope equipped with a Nikon DS-Ri2 camera and NIS-ELEMENTS software. The expression pattern of the DR5::VENUS reporter was detected by a Stereomicroscope, as described before (Shani et al., 2010; Bar et al., 2016) . For scanning electron microscopy (SEM), tissues were fixed in 30% Ethanol and vacuumed for 10 min, followed by dehydration in an increasing ethanol series up to 100% ethanol. Fixed tissues were critical-point dried, mounted on a copper plate and coated with gold. Samples were viewed using a JEOL 5410 LV microscope (Tokyo, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!