Zetapals zeta potential analyzer

Manufactured by Brookhaven Instruments

Sourced in United States

The ZetaPALS zeta potential analyzer is a laboratory instrument used to measure the zeta potential of particles suspended in a liquid. It determines the electrostatic charge on the surface of particles, which is a key parameter in understanding the stability and behavior of colloidal systems.

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Lab products found in correlation

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37 protocols using zetapals zeta potential analyzer

Physicochemical Characterization of Solid Lipid Nanoparticles

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Particle size analysis was conducted by dynamic light scattering (DLS), using a particle size analyzer (Brookhaven Instruments, Holtsville, NY, USA). Mean hydrodynamic diameter (Z-average), the size distribution and polydispersity index (PDI) of the nanoparticles in suspension were assessed by this technique. Zeta potential was determined by electrophoretic light scattering (ELS) using a ZetaPALS zeta potential analyzer (Brookhaven Instruments, Holtsville, NY, USA). Prior to the measurements, all samples were diluted (1:200) in PBS (pH 7.4) to yield a suitable scattering intensity. The average count rate was always between 100 and 500 kcps, showing that the dilution applied to the formulations was appropriate. The Z-average, PDI and zeta potential were obtained by calculating the average of ten runs of three independent batches of SLNs.

Neves A.R., Queiroz J.F, & Reis S. (2016). Brain-targeted delivery of resveratrol using solid lipid nanoparticles functionalized with apolipoprotein E. Journal of Nanobiotechnology, 14, 27.

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Superparamagnetic Sphere Characterization

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A double-sided gasket cut from 3M Tape (467MP with 200MP adhesive, Maple, MN) of 0.5 cm inner diameter was affixed to the top surface of a Dove prism (Thorlabs, PS991, Newton, NJ). A 10 μL sample of 8.3 μm superparamagnetic spheres (COMPEL COOH Glacial Blue, UMGB003-UMC4f, Fishers, IN) of density 1.1–1.2 g/mL suspended in deionized (DI) water, resistivity = 18.2 mΩ/cm, was injected into the gasket well and sealed with a coverslip. These particles were chosen for their large size, which minimizes the contribution of Brownian fluctuations on the measured gap width. To determine particle surface roughness, particle suspensions were dried on glass slides, and an atomic force microscopy (AFM) (Asylum Research, Santa Barbara, CA) was used in air tapping mode with a silicon probe with a resonant frequency of 190 kHz and a spring constant of 48 N/m (Tap190E-G, BudgetSensors, Sofia, Bulgaria). Zeta potential was measured with a ZetaPALS zeta potential analyzer (Brookhaven Instruments, Long Island, NY).

Disharoon D., Neeves K.B, & Marr D.W. (2019). ac/dc Magnetic Fields for Enhanced Translation of Colloidal Microwheels. Langmuir : the ACS journal of surfaces and colloids, 35(9), 3455-3460.

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Physicochemical Characterization of TEs

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The physicochemical properties of TEs were assessed with a ZetaPALS zeta potential analyzer (Brookhaven Instruments Corporation; Holtsville, NY, USA). Particle size (PS), polydispersity index (PDI), and zeta potential (ZP) were obtained after 5× times dilution in water. For particle size measurements, six runs of 2 min were performed at room temperature while for zeta potential six runs of 10 cycles were performed at a scattering angle of 90°.

Basto R., Andrade R., Nunes C., Lima S.A, & Reis S. (2021). Topical Delivery of Niacinamide to Skin Using Hybrid Nanogels Enhances Photoprotection Effect. Pharmaceutics, 13(11), 1968.

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Formulation and Characterization of β-CD-(D3)7/MMP-9-siRNA Complexes

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The β-CD-(D₃)₇/MMP-9-siRNA complexes were prepared at various charge ratios by mixing equal volumes of β-CD-(D₃)₇ with MMP-9-siRNA in phosphate-buffered saline. The charge ratio (amino group to phosphate group [N/P]) was calculated as a ratio of the number of primary amines in the polymer to the number of anionic phosphate groups in the MMP-9-siRNA. The samples were then vortexed and incubated at 37°C for 15 minutes to ensure formation of the complex. For the resultant β-CD-(D₃)₇/MMP-9-siRNA complexes, their particle sizes in aqueous system were measured at 25°C using a ZetaPALS zeta potential analyzer (Brookhaven Instruments Corporation, Holtsville, NY, USA). Their morphology was observed using transmission electron microscopy (TEM) (JEM2010; JEOL, Tokyo, Japan). Briefly, one drop of β-CD-(D₃)₇/MMP-9-siRNA complex suspension was placed on a copper grid and incubated for 60 seconds. The sample was negatively stained with 2% (w/v) uranyl acetate solution for 60 seconds. Excess liquid was removed by blotting with filter paper.

Li N., Luo H.C., Yang C., Deng J.J., Ren M., Xie X.Y., Lin D.Z., Yan L, & Zhang L.M. (2014). Cationic star-shaped polymer as an siRNA carrier for reducing MMP-9 expression in skin fibroblast cells and promoting wound healing in diabetic rats. International Journal of Nanomedicine, 9, 3377-3387.

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Investigating SDF-1α Interaction with Silicate Nanodisks

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Silicate nanodisks were dispersed in Milli-Q water at a concentration of 1% w/v and vigorously vortexed to ensure the formation of a clear suspension. To monitor the interaction between SDF-1α and nSi, the SDF-1α solution was added to the nSi suspension to achieve a final concentration of 50 ng/mL of the chemokine. The zeta potential of the samples was determined by a ZetaPALS zeta potential analyzer (Brookhaven Instruments Corporation, USA).

Basu S., Alkiswani A.R., Pacelli S, & Paul A. (2019). Nucleic Acid-Based Dual Cross-Linked Hydrogels for in Situ Tissue Repair via Directional Stem Cell Migration. ACS applied materials & interfaces, 11(38), 34621-34633.

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Nanoparticle Physicochemical Characterization

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The produced NPs were characterized for particle size, size distribution (polydispersity index), and zeta potential. Mean hydrodynamic diameter and polydispersity index were assessed by dynamic light scattering using a 90 Plus particle size analyzer (Brookhaven Instruments Corporation, Holtsville, NY, USA) and zeta potential was determined by phase analysis light scattering using a ZetaPALS zeta potential analyzer (Brookhaven) at 660 nm, with a detection angle of 90° at 25°C. All samples were diluted in water to a suitable scattering intensity and measurements were performed with three independent batches of NPs (six runs, ten cycles each).

Moura C.C., Segundo M.A., Neves J.D., Reis S, & Sarmento B. (2014). Co-association of methotrexate and SPIONs into anti-CD64 antibody-conjugated PLGA nanoparticles for theranostic application. International Journal of Nanomedicine, 9, 4911-4922.

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Characterization of Nanoparticle Formulations

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The hydrodynamic size distribution, polydispersity (PDI), and the surface charge (ζ-potential) of the developed formulations were characterized using dynamic and electrophoretic light scattering using a ZetaPALS/ZetaPotential Analyzer (Brookhaven Instruments, Holtsville, NY, USA), operating at a scattering angle of 90°, at 20 °C, with a dust cut-off set to 30. Prior to the measurements, formulations were diluted (1:100) in double-distilled water and filtered with a syringe filter (800 nm). For mean hydrodynamic diameter and PDI, six runs of 2 min were performed at each measurement. For ζ-potential determination, ten runs with ten cycles were performed at each measurement. All measurements were done in triplicate, and results were expressed as mean ± standard deviation (SD).

Magalhães J., L. Chaves L., C. Vieira A., G. Santos S., Pinheiro M, & Reis S. (2020). Optimization of Rifapentine-Loaded Lipid Nanoparticles Using a Quality-by-Design Strategy. Pharmaceutics, 12(1), 75.

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Characterization of Nanoformulations

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The mean size, polydispersity index (PDI), and zeta potential of the formulations were determined using a ZetaPALS zeta potential analyzer (Brookhaven Instruments Corporation; Holtsville, NY, USA). In particle size measurements, six runs of 2 min were performed at room temperature for each assay. The zeta potential of the nanoformulations was determined using an electrode operating at a scattering angle of 90° at room temperature. For each assay, six runs of 10 cycles were performed.

Barbosa A.I., Costa Lima S.A, & Reis S. (2019). Application of pH-Responsive Fucoidan/Chitosan Nanoparticles to Improve Oral Quercetin Delivery. Molecules, 24(2), 346.

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Zeta Potential Measurements of M. luteus

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Zeta potential measurements were performed using the ZetaPALS Zeta Potential Analyzer (Brookhaven Instruments Corporation), as described previously [24 (link)]. M. luteus was suspended in 1 X PBS buffer at 1•10⁹ cells/ml. The influence of CHX-hydrochloride on the zeta potential was studied over a range of concentrations from 0μg/ml to 1000μg/ml CHX.

Wendel S.O., Menon S., Alshetaiwi H., Shrestha T.B., Chlebanowski L., Hsu W.W., Bossmann S.H., Narayanan S, & Troyer D.L. (2015). Cell Based Drug Delivery: Micrococcus luteus Loaded Neutrophils as Chlorhexidine Delivery Vehicles in a Mouse Model of Liver Abscesses in Cattle. PLoS ONE, 10(5), e0128144.

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Nanoparticle Characterization by DLS, Physisorption, and TEM

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The hydrodynamic diameter and zeta potential of the nanoparticles were measured by dynamic light scattering (DLS, Brookhaven BIC ZetaPals), with a 35 mW 660 nm laser at room temperature. The zeta potential was measured in ultrapure water with a Brookhaven ZetaPALS Zeta-Potential Analyzer (Holtsville, NY). Particle porosity and surface area were measured with N₂ Physisorption (Micromeritics ASAP 2020, GA, USA). Samples were then analyzed under the ASAP 2020 analysis port at cryogenic temperatures. The surface area and pore size of samples were determined by the BJH method. Electron micrographs of the UMNs and PERFUMNs were obtained via TEM (FEI Tecnai 12, Houston, TX, USA) and Cryo-TEM (Tecnai G2 Spirit Biotwin, Houston, TX, USA) respectively. In TEM, 3 µL of UMNs were dried on Formvar-coated carbon grids overnight. For Cryo-TEM, 3 µL of PERFUMNs were placed on lacey carbon grids which were immediately dried with filter paper for 5 seconds at 100% humidity. The samples were vitrified by submerging grids into liquid ethane. Electron micrographs were obtained at 120 kV with an emission current of 4 mA.

Lee A.L., Gee C.T., Weegman B.P., Einstein S.A., Juelfs A., Ring H.L., Hurley K.R., Egger S.M., Swindlehurst G., Garwood M., Pomerantz W.C, & Haynes C.L. (2017). Oxygen Sensing with Perfluorocarbon-Loaded Ultraporous Mesostructured Silica Nanoparticles. ACS nano, 11(6), 5623-5632.

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