Matrigel matrix high concentration

Manufactured by Corning

Sourced in United States

Matrigel Matrix High Concentration is a protein mixture derived from Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells. It is a basement membrane extract that provides a natural extracellular matrix for the growth and differentiation of cells in vitro. The high concentration formulation offers a more defined and reproducible environment for cell culture and tissue engineering applications.

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Lab products found in correlation

Sonosite 180, by Fujifilm (2 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Adsc bulletkit, by Lonza (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Athymic nude nu nu mice, by Jackson ImmunoResearch (1 mentions) Female balb c nude mice, by Oriental Yeast (1 mentions) D luciferin, by GoldBio (1 mentions) Ivis spectrum imaging system, by PerkinElmer (1 mentions) Living image software, by PerkinElmer (1 mentions) Athymic nude mice, by Jackson ImmunoResearch (1 mentions)

Spelling variants of this product

Matrigel (8653 citations) Matrigel matrix (1030 citations) Matrigel Basement Membrane Matrix High Concentration (19 citations) Matrigel High Concentration (9 citations) Matrigel Matrix High Concentration (HC (3 citations) Matrigel GFR High Concentration (HC) Basement Membrane Matrix (2 citations)

10 protocols using matrigel matrix high concentration

Murine Hybridoma Cells for Erythrocyte Targeting

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10F7MN cells are murine hybridoma cells that secrete anti-human glycophorin A (CD235a) antibody, recognizing human erythrocytes. Details of 10F7MN cells are described in the Supplemental Material and Methods (SDC, http://links.lww.com/TXD/A213).
For transplantation of 10F7MN cells, B6 (allogeneic) and Balb (syngeneic) mice were used as hosts. To create a viscous gel suspension, 1 × 10⁶ 10F7MN cells were mixed with Matrigel matrix high concentration (Corning) and transplanted subcutaneously. Membranes were obtained from syngeneic, allogeneic, and third-party pregnant dams, and transplantations were carried out for following conditions: (a) 10F7MN cells only, (b) intact membranes only, (c) 10F7MN cells + intact membranes, (d) 10F7MN cells + digested membranes, and (e) Matrigel only. The progress of the tumors was monitored for 3 weeks after 10F7MN cell transplantation. Serum samples were collected from the transplanted mice at 1 and 3 weeks’ time points to determine the presence of anti-human CD235a antibody.

Togarrati P.P., Dinglasan N., Yee E., Heitman J.W., Jackman R.P., Geisberg M., Norris P.J., Bárcena A, & Muench M.O. (2019). Potential of Membranes Surrounding the Fetus as Immunoprotective Cell-Carriers for Allogeneic Transplantations. Transplantation Direct, 5(6), e460.

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Teratoma Assay for Stem Cell Differentiation

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For teratoma assays, the mouse and human iLEC were transfected with piggyBac GFP (either pBCAG-GFP or pBCAG-H2B-GFP and pBase plasmids, courtesy of Dr. Andras Nagy, Samuel Lunenfeld Research Institute, Toronto) using lipofectamine 3000 (Invitrogen) and FACS-sorted for GFP-high expression prior to injection. All cells were washed with PBS once and suspended into 200 ul of 66% DMEM (Thermo Fisher CAT# 11960044) + 33% Matrigel Matrix High Concentration (Corning Cat#354248). The 200 ul mixture was injected subcutaneously into each dorsal flank of NOD-SCID gamma (NSG) mouse, (NOD-SCID IL2Rg^null, JAX 005557). Palpable masses were formed 5–10 weeks after injection. To challenge the differentiation potential of iLEC, human (line CA1) or mouse (line R1) embryonic stem cells (ES) were co-transplanted with iLEC donors (1:4, 2 × 10⁶ ES: 8 × 10⁶ donor cells). As controls, 2.5 × 10⁶ human parental fibroblast or 8 × 10⁶ mouse embryonic fibroblast were suspended in 200 ul DMEM/matrigel mix, and injected subcutaneously into the contralateral dorsal flank of NSG mice. No palpable masses were formed during 10–16 weeks after injection. For mice with tumors, the animals were euthanized before the tumors reached endpoint size (1700 mm3), and tumors were dissected out and fixed in 10% formalin and paraffin-embedded prior to sectioning (8 μm thick) for H&E and IF staining. N = 2 for each cell line.

Wong A.P., Shojaie S., Liang Q., Xia S., Di Paola M., Ahmadi S., Bilodeau C., Garner J., Post M., Duchesneau P., Waddell T.K., Bear C.E., Nagy A, & Rossant J. (2019). Conversion of human and mouse fibroblasts into lung-like epithelial cells. Scientific Reports, 9, 9027.

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Tumor Growth Inhibition Experiments

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Four different tumor growth inhibition experiments were performed in this study. In all cases, initial tumor volumes for each group averaged ~300-400 mm³, calculated as Volume = (Length x Width x Depth)/2. Digital calipers were used to measure the greatest diameter of the tumor (length), the greatest diameter in the perpendicular dimension (width), and the greatest thickness of the tumor in the direction perpendicular to both length and width (depth). To measure depth, mice were first restrained by the tail while standing on their cage. The tumor was rocked to the side using the hand holding the tail, and the other hand was used to measure the tumor's depth using the digital calipers. This method was facile and reproducible. Five million UMUC3 cells and 2.5 million 3T3 cells were inoculated in a total volume of 100 μL and in a 1:3 matrigel:PBS solution (Corning Matrigel Matrix, High Concentration). Tumor volumes were measured daily. In groups receiving CA4P, CA4P was always dissolved in PBS and administered i.p. in a total of 200 ul. Groups receiving ¹⁷⁷Lu-LCP always received a dose of 250 microCuries (μCi) administered i.v. via the tail vein. Groups receiving ⁹⁰Y-LCP always received a dose of 125 μCi administered i.v. via the tail vein. Groups receiving a combination therapy always received CA4P 3 h before LCP.

Satterlee A.B., Rojas J.D., Dayton P.A, & Huang L. (2017). Enhancing Nanoparticle Accumulation and Retention in Desmoplastic Tumors via Vascular Disruption for Internal Radiation Therapy. Theranostics, 7(2), 253-269.

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3D iADSC Sheets for Cell Transplant

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Biocompatible nonwoven fabric sheets (ORTHOREBIRTH, Yokohama, Japan), which are made of fibers comprising biodegradable poly(lactic-co-glycolic acid) and hydroxyapatite [13 (link)], were cut into 3 mm squares and placed one per well in a Nunclon Sphera 96F Bottom Plate. After each well was filled with ADSC-BulletKit (Lonza), plates were degassed in a vacuum desiccator to remove air bubbles from the sheets and then preincubated for 1 h in a humidified incubator with 5% CO₂. iADSCs-mCherry-nls (4 × 10⁴) cells were added per well and cultured at 33 °C for 7–14 days. Sheets harboring the iADSCs were then removed and dipped into Matrigel Matrix High Concentration (Corning, Corning, NY, USA) before transplantation. Resulting sheets are referred to as 3D iADSC sheets. For the negative control, fabric sheets were degassed, precultured, and dipped into Matrigel without cell culture.

Ichise T., Ichise H, & Shimizu Y. (2024). Development of a Mouse Experimental System for the In Vivo Characterization of Bioengineered Adipose-Derived Stromal Cells. Cells, 13(7), 582.

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Teratoma Formation from Mouse ESCs

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Matrigel matrix high concentration (Corning, 354248) was diluted 1:1 in ice-cold DMEM and kept on ice. mESCs were treated with Trypsin-EDTA at 37 °C, resuspended in ESC media, centrifuged and washed once with PBS. Then, 5 × 10⁶ mESCs were diluted in 100 μl of ice-cold Matrigel:DMEM mixture and injected subcutaneously at the back of the neck or each dorsal flank (two separate injections) for teratoma assays (5 × 10⁶ mESCs in 100 μl at each injection site). Mice were anaesthetized during injections with isofluorane to allow for the cell/Matrigel mixture to gel in place. Palpable teratomas developed 10–40 days after injection, depending on the strain. Teratoma size was measured with calipers and the volume calculated using the formula V = (L×W×H)π/6. At endpoint, mice were euthanized and the teratomas excised and prepared for immunohistochemistry and histology.

Harding J., Vintersten-Nagy K., Yang H., Tang J.K., Shutova M., Jong E.D., Lee J.H., Massumi M., Oussenko T., Izadifar Z., Zhang P., Rogers I.M., Wheeler M.B., Lye S.J., Sung H.K., Li C., Izadifar M, & Nagy A. (2023). Immune-privileged tissues formed from immunologically cloaked mouse embryonic stem cells survive long term in allogeneic hosts. Nature Biomedical Engineering, 8(4), 427-442.

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Subcutaneous Tumor Xenograft Model

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Four week old male Nu/Nu athymic nude mice (J:NU 007850, Jackson labs, Bar Harbor, ME, USA) were purchased and allowed to acclimate for one week. Tumors were established by subcutaneous injection of 1.0×10⁶ DU145 cancer cells using a 28 gauge needle. A 200 µL bolus containing cells in PBS (Thermo Fisher Scientific) mixed in a 1:1 volume ratio with Matrigel Matrix High Concentration (Corning, Corning, NY, USA) was injected on left and right flanks of each animal. Tumor dimensions were measured daily using a digital caliper and a SonoSite 180 plus diagnostic ultrasound imaging system (SonoSite, Bothell, WA, USA). Tumor volumes were calculated using the formula V = π/6 × 2a × b, where a is the short axis and b the long axis of the tumor. When the volumes reached approximately 200 mm³ (roughly 3 weeks post injection), the mice were randomly assigned to groups and treatments commenced. A total of 10 tumors per group were tested.

Murad H.Y., Yu H., Luo D., Bortz E.P., Halliburton G.M., Sholl A.B, & Khismatullin D.B. (2019). Mechanochemical disruption suppresses metastatic phenotype and pushes prostate cancer cells toward apoptosis. Molecular cancer research : MCR, 17(5), 1087-1101.

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ALKBH4 shRNA in Xenograft Mice

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Female BALB/c nude mice were obtained from Oriental Yeast Corporation (Tokyo, JAPAN). Five-week-old mice were used for ALKBH4 shRNA stable cell-xenograft experiments. Animals were kept under 12 h light–dark cycles at 22–24 °C. A549 cells, which had been stably transfected with ALKBH4 shRNA (A549-shALKBH4) and control shRNA (A549-shControl), were both adjusted to a concentration of 0.6 × 10⁷ cells in 100 μL of serum-free DMEM. The cell suspensions, together with 100 μL of Matrigel Matrix High Concentration (Corning, New York, USA) were then injected subcutaneously into the right flanks of BALB/c nude mice (A549-shALKBH4, n = 8; A549-shControl, n = 8). One xenografted mouse, which was inoculated with A549-shALKBH4, died during the experiment. The tumour volume was calculated as follows: (tumour length × tumour width²)/2. All procedures were performed under a protocol approved by the Animal Experimentation Committee at Osaka University. All methods were carried out in accordance with relevant ARRIVE guidelines and regulations. We confirmed that all methods were carried out in accordance with relevant guidelines and regulations. Developed tumours were resected 52 days after xenografts.

Jingushi K., Aoki M., Ueda K., Kogaki T., Tanimoto M., Monoe Y., Ando M., Matsumoto T., Minami K., Ueda Y., Kitae K., Hase H., Nagata T., Harada-Takeda A., Yamamoto M., Kawahara K., Tabata K., Furukawa T., Sato M, & Tsujikawa K. (2021). ALKBH4 promotes tumourigenesis with a poor prognosis in non-small-cell lung cancer. Scientific Reports, 11, 8677.

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Teratoma formation and stabilization

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Matrigel Matrix High Concentration (Corning) was diluted 1:1 in cold DMEM and kept on ice. mESCs were dissociated, resuspended in mESC media, centrifuged, and washed twice in DMEM. Then, 5 million mESCs were diluted in 100 μl of the Matrigel-DMEM mixture and injected subcutaneously into the dorsal flank of the mice. Mice were anaesthetized during injections with isoflurane. Animals were randomly placed into control and treatment groups without specific methodology. Most teratomas formed 1–2-weeks following cell transplantation. Tissue size was measured using calipers and volume was calculated using the formula V = (L * W * H)*(π/6). All injected mESCs contained the FailSafe™ system (HSV-TK linked to Cdk1 expression) [42 (link)] and thus teratomas were stabilized with ganciclovir (GCV) (Fresenius Kabi C315110) daily or every other day through intraperitoneal injections at 50 mg/kg in PBS. GCV treatment durations varied depending on teratoma volume and mouse strain and are indicated in the figures. Mouse plasma was obtained from peripheral blood, collected by the tail vein in 7.5% EDTA-coated tubes (Covidien), before cell transplantation and weekly thereafter.

Sawula E., Miersch S., Jong E.D., Li C., Chou F.Y., Tang J.K., Saberianfar R., Harding J., Sidhu S.S, & Nagy A. (2023). Cell-based passive immunization for protection against SARS-CoV-2 infection. Stem Cell Research & Therapy, 14, 318.

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Orthotopic Liver Tumor Xenograft Model

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All in vivo procedures were approved and overseen by the Johns Hopkins Institutional Animal Care and Use Committee. Female athymic nude mice (NU/J) were purchased from the Jackson Laboratory (Bar Harbor, ME) and were implanted with orthotopic liver tumors at 6 to 8 weeks of age. Hep3b cells with or without constitutive firefly luciferase expression (fLuc+) were resuspended in a 1:1 mixture of Hanks’ balanced salt solution and Corning Matrigel Matrix High Concentration (Corning, catalog no. 354248) at 50 million cells/ml. Before implantation, animals were anesthetized with 2.5% isoflurane in oxygen, and the skin was cleaned with povidone-iodide and ethanol. An incision was made with a scalpel extending caudally from the xiphoid process. The left lateral liver lobe was visualized, and 1 million (20 μl) cells were injected under the liver capsule. Successful inoculation with cancer cells was be verified by pale, white protrusion at the point of injection. fLuc+ Hep3b tumor growth was monitored by IVIS (in vivo imaging system) (IVIS Spectrum imaging system, PerkinElmer, Waltham, MA). d-Luciferin (150 mg/kg) (Gold Biotechnology, St. Louis, MO) was administered intraperitoneally to mice, and then, imaging was performed 8 min later. Images were analyzed across regions of interest using Living Image software (PerkinElmer, Waltham, MA).

Vaughan H.J., Zamboni C.G., Hassan L.F., Radant N.P., Jacob D., Mease R.C., Minn I., Tzeng S.Y., Gabrielson K.L., Bhardwaj P., Guo X., Francisco D., Pomper M.G, & Green J.J. (2022). Polymeric nanoparticles for dual-targeted theranostic gene delivery to hepatocellular carcinoma. Science Advances, 8(29), eabo6406.

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Hep3B Xenograft Tumor Model in Nu/Nu Mice

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The four-week-old male and female Nu/Nu athymic nude mice were purchased from Jackson labs (J:NU 007850, Bar Harbor, ME) and allowed one week of acclimation. A 200 μL bolus containing 1.0 × 10⁶ Hep3B cells in PBS and Matrigel Matrix High Concentration (Corning, Corning, NY) mixed in a 1:1 volume was subcutaneously injected on the left and right flanks of each animal via a 28-gauge needle. Digital calipers and a SonoSite 180+ ultrasound imaging system (SonoSite, Bothell, WA) were used to measure tumor dimensions. The tumor volume was calculated using the following formula: V = π/6 × 2a × b, where a is the short axis and b the long axis of the tumor. Once the tumor volume reached approximately 200 mm³, mice were randomly assigned to a specific treatment group. There was a total of 10 tumors per group.

Murad H.Y., Bortz E.P., Yu H., Luo D., Halliburton G.M., Sholl A.B, & Khismatullin D.B. (2019). Phenotypic alterations in liver cancer cells induced by mechanochemical disruption. Scientific Reports, 9, 19538.

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