Anti gabab r1

Protein (50 mg) obtained from mice brain or intestine was isolated on 10% Tris-Glycine polyacrylamide gels (Bio-rad Laboratories, Inc., Hercules, CA, USA) and transferred to Immobilon-P membranes (Millipore Corporation, Bedform, MA, USA). The membrane was incubated overnight at 4 ℃ with following antibodies: anti-GABAA-a2 (Abcam, #ab72445), anti-GABAB-R1 (Cell signaling, #3835), anti-5HT1A (Abcam, #ab85615), or GAPDH (Cell signaling, #5174). The membrane-bound antibodies were applied to secondary IgG antibody (Cell signaling, #5127) and signals were visualized using ECL Prime (GE Healthcare Life Sciences, Issaquah, WA, USA) on a FluorChem E Imaging System (Protein Simple, San Jose, CA, USA). Protein signals were quantified using FluorChem E Image Quant software with normalization by GAPDH as an internal control.

MPTP and MPP⁺ were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ginsenoside Rb1 was purchased from MUST Biotechnology (Chengdu, China). Anti-GABA_ARα1 was purchased from Millipore (Bedford, MA, USA). Antibodies for anti-vGAT, anti-integrin, as well as the GABA_B-receptor agonist Baclofen, were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-gephyrin, anti-PSD-95, and anti-GABA_BR1 were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-GAD67 was purchased from Thermo Scientific (Waltham, MA, USA). Anti-actin was purchased from Beyotime (Shanghai, China). Alexa Fluor 488-conjugated goat anti-mouse, Alexa Fluor 594-conjugated goat anti-rabbit, horseradish peroxidase (HRP)-conjugated goat anti-mouse and anti-rabbit antibodies were purchased from Multi Sciences (Hangzhou, China). EZ-Link Sulfo-NHS-SS-Biotin was purchased from Thermo Scientific (Waltham, MA, USA).