After 24 hours of reperfusion, venous blood from mice was collected through the posterior orbital venous plexus to determine the serum troponin T level using an ELISA kit for troponin T (Mito Sciences). Next, the mice heart tissues were harvested to detect IFN‐γ (ab252352; Abcam), TNF‐α (ab208348; Abcam), IL‐4 (ab221833; Abcam) and IL‐13 (ab219634; Abcam) protein levels using an ELISA kit. The myeloperoxidase (MPO) level was detected using an MPO activity assay kit (ab105136; Abcam).
Ab105136
Manufactured by Abcam
Sourced in United Kingdom, United States
Ab105136 is a lab equipment product offered by Abcam. It is a reagent designed for use in scientific research applications. The core function of this product is to serve as a tool for researchers in their laboratory work, but no further details about its intended use or application can be provided in an unbiased and factual manner.
Automatically generated - may contain errors
Lab products found in correlation
Ab221833, by Abcam (1 mentions)
Ab208348, by Abcam (1 mentions)
Mpo activity assay kit, by Abcam (1 mentions)
Ab219634, by Abcam (1 mentions)
Bc0020, by Solarbio (1 mentions)
Ab100556, by Abcam (1 mentions)
Ab174443, by Abcam (1 mentions)
Ab178013, by Abcam (1 mentions)
Bc0170, by Solarbio (1 mentions)
Microplate reader, by Agilent Technologies (1 mentions)
Spectramax 340, by Molecular Devices (1 mentions)
Ab213906, by Abcam (1 mentions)
Ab100778, by Abcam (1 mentions)
Lxsarm, by R&D Systems (1 mentions)
Ab119558, by Abcam (1 mentions)
Ab118970, by Abcam (1 mentions)
Ab119585, by Abcam (1 mentions)
St51011, by R&D Systems (1 mentions)
Ab102523, by Abcam (1 mentions)
Ab102531, by Abcam (1 mentions)
15 protocols using ab105136
1
Cardiac Injury Biomarkers and Cytokine Profiling
2
Uterine MPO Activity Quantification
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The mouse uteri were weighed, and PBS was added according to a weight-to-volume ratio of 1:9 to make tissue homogenates with a glass homogenizer. After centrifugation, the supernatants were collected to measure MPO activity using an MPO kit (ab105136, Abcam, Cambridge, MA, USA) according to the manufacturer’s instructions.
3
Quantifying Myeloperoxidase Activity
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4
Neutrophil Recruitment Evaluation
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Myeloperoxidase (MPO) activity was measured to evaluate neutrophil recruitment using an MPO activity assay kit (ab105136, Abcam) according to the manufacturer’s instructions. The data are expressed as units per milligram of total protein.
5
Inflammatory Cytokines and Oxidative Stress in Colonic Tissue
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The supernatant of the cells was collected and centrifuged at 4 °C and 10,000 rpm for 5 min. Small sections (~ 1 cm) of excised distal colonic tissue were collected. Then, ~ 10 mg colon tissue was homogenized with 1 ml PBS and centrifuged at 16,000g for 20 min at 4 °C. The levels of IL-6 (ab178013, Abcam), IL-17 (ab100556, Abcam), IFN-γ (ab174443, Abcam), IL-10 (cat. no. EK0417, Boster Biological Technology), SOD (BC0170, Solarbio, Beijing, China), MDA (BC0020, Solarbio) and MPO (ab105136, Abcam) in the supernatant were detected according to the product instructions.
6
Myeloperoxidase Activity Assay
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Lung tissue homogenate was subjected to MPO assay using a commercial kit (cat. no. ab105136, Abcam) and the absorbance at 460 nm was detected using a microplate reader (Bio-Tek Instruments, Inc.).
7
Quantifying Lung Tissue MPO Activity
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Lung tissues were homogenized and MPO activity was detected using a commercially available kit (ab105136, Abcam, Cambridge, MA, USA). Absorbance at 412 nm was determined.
8
Colorimetric MPO Activity Assay
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For the measurement of MPO activity, colon specimens were weighed and homogenized in 4 volumes of assay buffer from the colorimetric MPO activity assay kit (ab105136, Abcam, Cambridge, UK). The supernatant was then collected via centrifugation at 13,000× g and 4 °C for 10 min. MPO activity was determined using the MPO activity assay kit according to the manufacturer’s instructions.
9
Measuring Lung Tissue MPO Activity
10
Measurement of Colon MPO Activity
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MPO activity was measured using an MPO activity assay kit (ab105136; Abcam, Cambridge, UK) according to the manufacturer's protocol. Briefly, the colons (10 mg) of mice euthanized on day 28 were homogenized with MPO assay buffer, and the sample was centrifuged at 13 000 × g for 10 min. After collecting the supernatant, the MPO activity assay was performed. MPO activity of the samples was calculated by measuring the absorbance at 412 nm using a microplate reader (SpectraMAX 340, Molecular Devices, San Jose, USA).
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