Standard laboratory chemicals and protease inhibitor cocktail (PIC) were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Research Products International (Mt. Prospect, IL, USA). Q-sepharose, Heparin and S200 size exclusion chromatography resins were from GE Healthcare (Pittsburgh, PA, USA). Ni2+-NTA agarose was from Gold Biotechnology (St. Louis, MO). Biogel-P4 resin was from Bio-Rad Laboratories (Hercules, CA, USA). Enzymes for molecular Biology were from New England Biolabs (Ipswich, MA, USA). Oligonucleotides were purchased from Integrated DNA Technologies (Coralville, IA). Fmoc-4-amino-phenylalanine was from Angene International Ltd. (Nanjing, China). Commercial 4-azidophenylalanine was purchased from Chem-Impex International (Wood Dale, IL, USA). MB543 DBCO was purchased from Click Chemistry Tools (Scottsdale, AZ, USA). Alexa Fluor 594 DIBO alkyne and BL21Ai cells were purchased from ThermoFisher Scientific (Waltham, MA, USA).
S200 size exclusion chromatography
Manufactured by GE Healthcare
Sourced in United States
The S200 size exclusion chromatography system is a laboratory instrument used for the separation and purification of biomolecules such as proteins, peptides, and nucleic acids based on their size and molecular weight. The system uses a porous stationary phase to separate the sample components, allowing larger molecules to elute first and smaller molecules to elute later. The S200 system provides a reliable and efficient method for the analysis and purification of a wide range of biological samples.
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Lab products found in correlation
Q sepharose, by GE Healthcare (1 mentions)
Heparin, by GE Healthcare (1 mentions)
Biogel p4 resin, by Bio-Rad (1 mentions)
Bl21 ai cells, by Thermo Fisher Scientific (1 mentions)
Protease inhibitor cocktail, by Merck Group (1 mentions)
Enzymes for molecular biology, by New England Biolabs (1 mentions)
Oligonucleotide, by Integrated DNA Technologies (1 mentions)
Porous q, by GE Healthcare (1 mentions)
Strep tactin, by Qiagen (1 mentions)
2 protocols using s200 size exclusion chromatography
1
Protein Purification Using Chromatography
2
Purification of S. cerevisiae Cohesin Complex
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S. cerevisiae cohesin (Smc1, Smc3, Rad21, and Scc3), Scc2-Scc4 complex, Pds5, and Wapl were amplified by polymerase chain reaction (PCR) using genomic DNA as templates and cloned into a modified pFBDM vector with a double Strep-tag II (ds) and a tobacco etch virus (TEV) cleavage site at the N terminus of Rad21, the C terminus of Scc2, the C terminus of Pds5, and the C terminus of Wapl respectively. The resultant protein expression cassettes were recombined with the DH10MultiBac cells to create the corresponding bacmids. S. cerevisiae cohesin was expressed using the baculovirus/insect cell (Sf21) systems whereas the Scc2-Scc4, Pds5, and Wapl were expressed using Hi5 insect cells. All proteins were purified by Strep-Tactin (Qiagen), anion exchange chromatography Porous Q, and S200 size-exclusion chromatography (GE Healthcare).
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