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His tagged cxcl16

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His-tagged CXCL16 is a recombinant protein with a histidine (His) tag. CXCL16 is a chemokine that plays a role in the immune system.

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Lab products found in correlation

Calcein, by Thermo Fisher Scientific (2 mentions) Mithras lb 940 multimode microplate reader, by Berthold Technologies (2 mentions) Recombinant mouse cxcl16, by Thermo Fisher Scientific (2 mentions) His tagged epcam, by Sino Biological (2 mentions) Calcein, by Berthold Technologies (2 mentions)

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CXCL16 (11 citations)

2 protocols using his tagged cxcl16

1

T cell adhesion to CXCL16 and EpCAM

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T cells were either stained with Calcein (Life Technologies, Carlsbad, CA, USA) or CSFE (Cat. Number C34554, ThermoScientific, Darmstadt) and pre-incubated with or without 9 pmol recombinant mouse CXCL16 (Cat. Number 250-28, Peprotech, London, UK). Nickel-coated 96-well plates (Cat. Number 15442, ThermoScientific, Darmstadt) were coated with 9 pmol His-tagged CXCL16 (Cat. Number 50142-M08H, SinoBiological, Peking, China), 9 pmol His-tagged EpCAM (Cat. Number 50591-M08H, SinoBiological, Peking, China) or 9 pmol BSA. The pre-stimulated T cells were transferred to the CXCL16- or BSA-coated Nickel plate. After 25 minutes incubation and a washing step, adherent cells were lysed using RIPA buffer. Calcein or CSFE was detected with the Mithras LB 940 Multimode Microplate Reader (Berthold Technologies, Bad Wildbad), where the fluorescent signal intensity is proportional to the quantity of adherent cells.
Lesch S., Blumenberg V., Stoiber S., Gottschlich A., Ogonek J., Cadilha B.L., Dantes Z., Rataj F., Dorman K., Lutz J., Karches C.H., Heise C., Kurzay M., Larimer B.M., Grassmann S., Rapp M., Nottebrock A., Kruger S., Tokarew N., Metzger P., Hoerth C., Benmebarek M.R., Dhoqina D., Grünmeier R., Seifert M., Oener A., Umut Ö., Joaquina S., Vimeux L., Tran T., Hank T., Baba T., Huynh D., Megens R.T., Janssen K.P., Jastroch M., Lamp D., Ruehland S., Di Pilato M., Pruessmann J.N., Thomas M., Marr C., Ormanns S., Reischer A., Hristov M., Tartour E., Donnadieu E., Rothenfusser S., Duewell P., König L.M., Schnurr M., Subklewe M., Liss A.S., Halama N., Reichert M., Mempel T.R., Endres S, & Kobold S. (2021). T cells armed with the C-X-C chemokine receptor type 6 enhance adoptive cell therapy for pancreatic tumours. Nature biomedical engineering, 5(11), 1246-1260.
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2

T cell adhesion to CXCL16 and EpCAM

Check if the same lab product or an alternative is used in the 5 most similar protocols
T cells were either stained with Calcein (Life Technologies, Carlsbad, CA, USA) or CSFE (Cat. Number C34554, ThermoScientific, Darmstadt) and pre-incubated with or without 9 pmol recombinant mouse CXCL16 (Cat. Number 250-28, Peprotech, London, UK). Nickel-coated 96-well plates (Cat. Number 15442, ThermoScientific, Darmstadt) were coated with 9 pmol His-tagged CXCL16 (Cat. Number 50142-M08H, SinoBiological, Peking, China), 9 pmol His-tagged EpCAM (Cat. Number 50591-M08H, SinoBiological, Peking, China) or 9 pmol BSA. The pre-stimulated T cells were transferred to the CXCL16- or BSA-coated Nickel plate. After 25 minutes incubation and a washing step, adherent cells were lysed using RIPA buffer. Calcein or CSFE was detected with the Mithras LB 940 Multimode Microplate Reader (Berthold Technologies, Bad Wildbad), where the fluorescent signal intensity is proportional to the quantity of adherent cells.
Lesch S., Blumenberg V., Stoiber S., Gottschlich A., Ogonek J., Cadilha B.L., Dantes Z., Rataj F., Dorman K., Lutz J., Karches C.H., Heise C., Kurzay M., Larimer B.M., Grassmann S., Rapp M., Nottebrock A., Kruger S., Tokarew N., Metzger P., Hoerth C., Benmebarek M.R., Dhoqina D., Grünmeier R., Seifert M., Oener A., Umut Ö., Joaquina S., Vimeux L., Tran T., Hank T., Baba T., Huynh D., Megens R.T., Janssen K.P., Jastroch M., Lamp D., Ruehland S., Di Pilato M., Pruessmann J.N., Thomas M., Marr C., Ormanns S., Reischer A., Hristov M., Tartour E., Donnadieu E., Rothenfusser S., Duewell P., König L.M., Schnurr M., Subklewe M., Liss A.S., Halama N., Reichert M., Mempel T.R., Endres S, & Kobold S. (2021). T cells armed with the C-X-C chemokine receptor type 6 enhance adoptive cell therapy for pancreatic tumours. Nature biomedical engineering, 5(11), 1246-1260.
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