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Human nag 1

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Human NAG-1 is a recombinant protein that functions as a growth and differentiation factor. It is produced in a human cell expression system.

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Lab products found in correlation

Non esterified free fatty acid kit, by Sekisui (4 mentions) Serum separator tubes, by Sarstedt (3 mentions) Mouse igf 1, by R&D Systems (3 mentions) Mouse glycerol, by Cayman Chemical (2 mentions) Cholesterol and triglycerides kit, by Beckman Coulter (2 mentions) Au400e clinical analyzer, by Beckman Coulter (2 mentions)

3 protocols using human nag 1

1

Serum Biomarker Measurement in Mice

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Terminal bleeds from mice were incubated at room temperature for 1 hour in serum separator tubes (Sarstedt, Nümbrecht, Germany) and then spun at 10,000 × rpm for 5 minutes. Serum was collected and stored at −80°C until analysis. Mouse leptin, human NAG-1, mouse IGF-1 (R&D, Minneapolis, MN), mouse insulin (Alpco, Salem, NH), and mouse glycerol (Cayman, Detroit, MI) ELISA kits was used according to manufacturer’s instructions. Cholesterol and triglycerides kit was purchased from Beckman Coulter (Melvill, NY) and non-esterified free fatty acid kit was purchased from Sekisui Diagnostics (Exton, PA). Analysis was done using an Olympus AU400e clinical analyzer by Beckman Coulter.
Chrysovergis K., Wang X., Kosak J., Lee S.H., Kim J.S., Foley J.F., Travlos G., Singh S., Baek S.J, & Eling T.E. (2014). NAG-1/GDF15 prevents obesity by increasing thermogenesis, lipolysis and oxidative metabolism. International journal of obesity (2005), 38(12), 1555-1564.
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2

Serum Biomarker Measurement in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Terminal bleeds from mice were incubated at room temperature for 1 hour in serum separator tubes (Sarstedt, Nümbrecht, Germany) and then spun at 10,000 × rpm for 5 minutes. Serum was collected and stored at −80°C until analysis. Mouse leptin, human NAG-1, mouse IGF-1 (R&D, Minneapolis, MN), mouse insulin (Alpco, Salem, NH), and mouse glycerol (Cayman, Detroit, MI) ELISA kits was used according to manufacturer’s instructions. Cholesterol and triglycerides kit was purchased from Beckman Coulter (Melvill, NY) and non-esterified free fatty acid kit was purchased from Sekisui Diagnostics (Exton, PA). Analysis was done using an Olympus AU400e clinical analyzer by Beckman Coulter.
Chrysovergis K., Wang X., Kosak J., Lee S.H., Kim J.S., Foley J.F., Travlos G., Singh S., Baek S.J, & Eling T.E. (2014). NAG-1/GDF15 prevents obesity by increasing thermogenesis, lipolysis and oxidative metabolism. International journal of obesity (2005), 38(12), 1555-1564.
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3

Serum Biomarker Profiling in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Terminal bleeds from mice were incubated at room temperature for 1 hour in serum separator tubes (Sarstedt, Nümbrecht, Germany) and then spun at 10,000 × rpm for 5 minutes. Serum was collected and stored at −80°C until analysis. Mouse leptin, mouse growth hormone, human NAG-1, mouse IGF-1 (R&D, Minneapolis, MN) and mouse insulin (Alpco, Salem, NH) ELISA kits were used according to manufacturer's instructions.
Wang X., Chrysovergis K., Kosak J., Kissling G., Streicker M., Moser G., Li R, & Eling T.E. (2014). hNAG-1 increases lifespan by regulating energy metabolism and insulin/IGF-1/mTOR signaling. Aging (Albany NY), 6(8), 690-704.
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