All stocks were raised on standard Drosophila media and crosses were performed at 25°C unless otherwise indicated. UAS-APLP1, UAS-APPLsd were kindly provided by Dr. Merdes [22 (link)]; pucH246, UAS-mmp1-IR, UAS-mmp2-IR, UAS-TIMP1, UAS-RasV12, UAS-Rho1-IR, UAS-p35, UAs-P53DN were obtained from Bloomington Stock Center; sd-Gal4, UAS-BskDN, UAS-Puc, UAS-Egr, UAS-Hep [70 (link)]; ptc-Gal4, en-Gal4, pucE69 34, UAS-LacZ [71 (link)] were previously described.
Sd gal4
Manufactured by Bloomington Drosophila Stock Center
The Sd-Gal4 is a genetic tool used in Drosophila research. It functions as a transcriptional activator, driving the expression of target genes in a spatially and temporally controlled manner. The Sd-Gal4 line is a valuable resource for studying gene function and developmental processes in the fruit fly model organism.
Automatically generated - may contain errors
Lab products found in correlation
En gal4, by Bloomington Drosophila Stock Center (2 mentions)
Ptc gal4, by Bloomington Drosophila Stock Center (2 mentions)
Uas bskdn, by Bloomington Drosophila Stock Center (1 mentions)
Uas hep, by Bloomington Drosophila Stock Center (1 mentions)
Uas rasv12, by Bloomington Drosophila Stock Center (1 mentions)
Uas puc, by Bloomington Drosophila Stock Center (1 mentions)
Uas lacz, by Bloomington Drosophila Stock Center (1 mentions)
Puch246, by Bloomington Drosophila Stock Center (1 mentions)
Uas mmp1 ir, by Bloomington Drosophila Stock Center (1 mentions)
Uas p35, by Bloomington Drosophila Stock Center (1 mentions)
Act gal4, by Bloomington Drosophila Stock Center (1 mentions)
Uas rpr, by Bloomington Drosophila Stock Center (1 mentions)
2 protocols using sd gal4
1
Drosophila Genetic Manipulations
2
Mosaic Transgene Expression in Drosophila Imaginal Discs
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Drosophila stocks were maintained by standard methods at 25°C. For generation of mosaic UAS-transgene overexpression clones in imaginal discs, first instar larvae (48 h after egg deposition) were heat-shocked for 15–60 min at 37°C. To control the timing of UAS-transgene overexpression with the temperature-sensitive Gal80 (Gal80ts) in imaginal discs, fly larvae were kept in 18°C until they reached second instar stage and were then transferred to 29°C. The following fly strains were used: ptc-Gal4 (Bloomington #2017), sd-Gal4 (Bloomington #8609), en-Gal4 (Bloomington #30564), act-Gal4 (Bloomington #4414), UAS-RhoGEF2-RNAi (Bloomington #34643), UAS-rpr (Bloomington #5823), UAS-lgl-RNAi (VDRC #51247), UAS-scrib-RNAi (VDRC #105412), UAS-STAT92E-RNAi (VDRC #106980), UAS-YkiM123 [51 (link)], UAS-3HA-STAT92EΔNΔC [27 (link)], 10xSTAT-GFP [22 (link)], and vkg-GFP (G00454). All genotypes of flies used in each experiment are described below.
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