Fragment analyzer auto capillary electrophoresis system

Manufactured by Agilent Technologies

Sourced in France

The Fragment Analyzer Auto Capillary Electrophoresis System is a lab equipment product from Agilent Technologies. It is an automated system that utilizes capillary electrophoresis technology to analyze and separate DNA, RNA, and protein fragments.

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Lab products found in correlation

Iscript cdna synthesis kit, by Bio-Rad (1 mentions) Nucleospin rna kit, by Macherey-Nagel (1 mentions) Fugene hd transfection reagent, by Promega (1 mentions)

Spelling variants of this product

Fragment Analyzer (884 citations) Fragment Analyzer system (61 citations) Capillary electrophoresis system (11 citations) Fragment Analyzer capillary electrophoresis system (2 citations)

2 protocols using fragment analyzer auto capillary electrophoresis system

Quantifying Rhodopsin Expression in Mutant midigenes

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HEK 293-T cells were transfected with 1.5 µg of WT or mutant midigene construct. After 24 hours, each well was trypsinized and subdivided in 5 wells of a 24-well plate. After reattachment, cells were transfected with the AON, the SON, or not treated (NT) (FuGENE HD Transfection Reagent, Promega, The Netherlands). After 48 hours, RNA was isolated (NucleoSpin RNA kit, Macherey-Nagel, Germany) followed by complementary DNA (cDNA) synthesis (iSCRIPT cDNA synthesis kit, Bio-Rad, Belgium). A PCR with 50 ng of cDNA was performed of which 10 µl was loaded on a 2% agarose gel (primers: Table S10). Rhodopsin was used as transfection and loading control. Two independent experiments were performed. Quantification of transcript ratios was done via a Fragment Analyzer Auto Capillary Electrophoresis System (Advanced Analytical Technologies, Inc., France).

Bauwens M., Garanto A., Sangermano R., Naessens S., Weisschuh N., De Zaeytijd J., Khan M., Sadler F., Balikova I., Van Cauwenbergh C., Rosseel T., Bauwens J., De Leeneer K., De Jaegere S., Van Laethem T., De Vries M., Carss K., Arno G., Fakin A., Webster A.R., de Ravel de l’Argentière T.J., Sznajer Y., Vuylsteke M., Kohl S., Wissinger B., Cherry T., Collin R.W., Cremers F.P., Leroy B.P, & De Baere E. (2019). ABCA4-associated disease as a model for missing heritability in autosomal recessive disorders: novel noncoding splice, cis-regulatory, structural, and recurrent hypomorphic variants. Genetics in Medicine, 21(8), 1761-1771.

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Quantifying Aberrant Transcripts via RT-PCR

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To quantify the ratios between correct and aberrant transcripts we loaded the RT-PCR samples onto a Fragment Analyzer Auto Capillary Electrophoresis System (Advanced Analytical Technologies, Inc.). Analysis of the peaks was performed using the corrected peak area parameter and only the peaks corresponding to the expected bands were taken into account.

Sangermano R., Garanto A., Khan M., Runhart E.H., Bauwens M., Bax N.M., van den Born L.I., Khan M.I., Cornelis S.S., Verheij J.B., Pott J.W., Thiadens A.A., Klaver C.C., Puech B., Meunier I., Naessens S., Arno G., Fakin A., Carss K.J., Raymond F.L., Webster A.R., Dhaenens C.M., Stöhr H., Grassmann F., Weber B.H., Hoyng C.B., De Baere E., Albert S., Collin R.W, & Cremers F.P. (2019). Deep-intronic ABCA4 variants explain missing heritability in Stargardt disease and allow correction of splice defects by antisense oligonucleotides. Genetics in Medicine, 21(8), 1751-1760.

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