Saracatinib

Manufactured by Cayman Chemical

Saracatinib is a tyrosine kinase inhibitor. It is used in research applications.

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Lab products found in correlation

Retinoic acid, by Merck Group (2 mentions) Antibody against gapdh, by Santa Cruz Biotechnology (2 mentions) Rhodamine conjugated transferrin, by Thermo Fisher Scientific (2 mentions) Boron dipyrromethene bodipy fl c5 laccer, by Thermo Fisher Scientific (2 mentions) Ski 1, by Merck Group (2 mentions) Doxorubicin, by Merck Group (2 mentions) Bafilomycin a1, by Merck Group (1 mentions) Ab138501, by Abcam (1 mentions) 5 fluorouracil, by Merck Group (1 mentions) P tyr 100, by Cell Signaling Technology (1 mentions) Flag m2 antibody, by Merck Group (1 mentions) Su6656, by Cayman Chemical (1 mentions) Phospho tyrosine p tyr 1000, by Cell Signaling Technology (1 mentions) Anti α sma, by Santa Cruz Biotechnology (1 mentions) Pf 573228, by Cayman Chemical (1 mentions) Anti fak, by Merck Group (1 mentions) Anti ctgf, by Abcam (1 mentions) Anti myc tag, by Abcam (1 mentions) Ehop 016, by Selleck Chemicals (1 mentions) Pf 562271, by Cayman Chemical (1 mentions)

5 protocols using saracatinib

Antibody Characterization for α-Synuclein Analysis

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Antibodies against cav-1 (#610407) and α-syn (#610786) were purchased from BD Biosciences (Franklin Lakes, NJ). Antibodies against human α-syn (#ab138501), Tuj-1 (#ab18207) and mCherry (#ab167453) were purchased from Abcam (Cambridge, MA). Antibodies against pCav-1 (#3251S), pSrc (Y416) (#6943), and c-Src (#2123) were purchased from Cell signaling Technology (Danvers, MA). Antibody against GAPDH (#SC-32233) was procured from Santa Cruz Biotechnology (Santa Cruz, CA). Rhodamine-conjugated transferrin (#T23365) and boron-dipyrromethene (BODIPY) FL C5-LacCer (#B34402) were purchased from Molecular Probes (Leiden, The Netherlands). Saracatinib (#11497) was procured from Cayman Chemical (Ann Arbor, MI). SKI-1 (c-Src inhibitor 1, #S2075), retinoic acid (RA) (#r2625), and bafilomycin A1 (#B1793) were obtained from Sigma (St. Louis, MO). Antibodies against Iba-1 (#019-19741) and pSer 129 α-syn (#015-25191) was purchased from Wako (Richmond, VA). Antibody against glial fibrillary acidic protein (GFAP) (#RA22101) was purchased from Neuromics (Montreal, QC). Recombinant α-syn monomers and fibrils were prepared as described previously [24 (link)]. The status of fibrils was determined by thioflavin T binding assay.

Ha T.Y., Choi Y.R., Noh H.R., Cha S.H., Kim J.B, & Park S.M. (2021). Age-related increase in caveolin-1 expression facilitates cell-to-cell transmission of α-synuclein in neurons. Molecular Brain, 14, 122.

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Epithelial and Colonic Injury Induction

Cited 1 time

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To induce epithelial injury, hydroxyurea, 5-fluorouracil, and doxorubicin (Sigma) were administered intraperitoneally at a dose of 1g/kg, 150mg/kg, and 15mg/kg, respectively. To induce colonic injury, 2% dextran sodium sulfate (DSS) was given for 5 days. For Lgr5⁺ cell ablation, DT was administered via intraperitoneal injection at a dose of 50ug/kg. Whole body irradiation at a dose of 10 or 12 Gy was given to some mice. Src inhibitor (Saracatinib, Caymanchemicals) and γ-secretase inhibitor dibenzazepine (DBZ, Tocris BioScience) were administered at indicated time points orally at a dose of 50mg/kg and intraperitonially at a dose of 5 mg/kg, respectively. Bone marrow transplantation and EdU label retention assay was performed as described previously^{23 (link), 24 (link)}.
More detailed information is described in Supplementary Methods.

Hayakawa Y., Tsuboi M., Asfaha S., Kinoshita H., Niikura R., Konishi M., Hata M., Oya Y., Kim W., Middelhoff M., Hikiba Y., Higashijima N., Ihara S., Ushiku T., Fukayama M., Tailor Y., Hirata Y., Guha C., Yan K.S., Koike K, & Wang T.C. (2018). BHLHA15-positive Secretory Precursor Cells Can Give Rise to Tumors in Intestine and Colon in Mice. Gastroenterology, 156(4), 1066-1081.e16.

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Tyrosine Phosphorylation and PDHA1 Mutants

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HEK293, SW480, SW620 and 4T1 cells were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% bovine calf serum. AsPC1 cells were cultured in RPMI-1640 medium with 10% fetal bovine serum (FBS). MCF-10A cells were grown in DMEM/F12 with 5% Horse Serum, 20ng/ml EGF, 0.5 mg/ml Hydrocortisone, 10μg/ml Insulin. Src inhibitors PP2, SU6656 and Saracatinib were purchased from Cayman Chemical, and chemodrug Doxorubicin from Sigma. Mouse and rabbit phospho-tyrosine (P-Tyr-1000 and P-Tyr-100) and PDHA1 monoclonal antibodies were from Cell Signaling. Mouse monoclonal Flag M2 antibody was from Sigma. PDHA1 AAA mutant was created by substituting three serine residues (S293, S300, and S232) with alanine. PDHA1 Y289F mutant was generated by replacing tyrosine 289 with phenylalanine. Mutagenesis was achieved using a method described in QuickChange II Site-Directed Mutagenesis (Stratagene).

Jin Y., Cai Q., Shenoy A.K., Lim S., Zhang Y., Charles S., Tarrash M., Fu X., Kamarajugadda S., Trevino J.G., Tan M, & Lu J. (2016). Src drives the Warburg effect and therapy resistance by inactivating pyruvate dehydrogenase through tyrosine-289 phosphorylation. Oncotarget, 7(18), 25113-25124.

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Pathway Inhibitors Modulate Cellular Signaling

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All three FAK family inhibitors (PF-431396, PF-562271, and PF-573228) and Pan-Src kinase inhibitor (Saracatinib) were purchased from Cayman Chemicals. Src kinase inhibitor PP2 and Smad3 inhibitor (SIS3) were purchased from Tocris of R&D Systems. ROCK inhibitor (Y-27632), Cdc42 GTPase inhibitor (ML-141), and Rac1/3 inhibitor (EHop-016) were purchased from Selleckchem. YAP-TEAD inhibitor (Verteporfin) was purchased from Cayman Chemicals (USA) and Selleckchem. Anti-FAK was purchased from Millipore. Antibodies against p-FAK(Y397), p-PYK2(Y402), PYK2, c-Src, p-Src(Y416), Smad2, p-Smad2(S465/467), p-Smad3(S423/425), Smad3, and YAP/TAZ were purchased from Cell Signaling Technology. anti-α-SMA and anti-actin were Sigma-Korea. Antibodies to detect p-PYK2(Y402), p-PYK2(Y579/580), and p-PYK2(Y881) were purchased from Invitrogen. Anti-CTGF and anti-myc-tag were purchased from Abcam. Anti-YAP and anti-α-SMA were purchased from Santa Cruz Biotechnology and Dako, respectively. Secondary antibodies to detect the primary antibodies were purchased as followed; HRP-conjugated anti-rabbit and anti-mouse from Pierce-Thermo Scientific, all Alexa-fluorophore conjugated antibodies from Invitrogen.

Kim J., Kang W., Kang S.H., Park S.H., Kim J.Y., Yang S., Ha S.Y, & Paik Y.H. (2020). Proline-rich tyrosine kinase 2 mediates transforming growth factor-beta-induced hepatic stellate cell activation and liver fibrosis. Scientific Reports, 10, 21018.

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Antibody Characterization and Protein Preparation

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Antibodies against cav-1 (#610407) and α-syn (#610786) were purchased from BD Biosciences (Franklin Lakes, NJ). Antibodies against human α-syn (#ab138501), and mCherry (#ab167453) were purchased from Abcam (Cambridge, MA). Antibody against pCav-1 (#3251S) was purchased from Cell signaling Technology (Danvers, MA). Antibody against GAPDH (#SC-32233) was procured from Santa Cruz Biotechnology (Santa Cruz, CA). Rhodamine-conjugated transferrin (#T23365) and boron-dipyrromethene (BODIPY) FL C5-LacCer (#B34402) were purchased from Molecular Probes (Leiden, The Netherlands).
Saracatinib (#11497) was procured from Cayman Chemical (Ann Arbor, MI). SKI-1 (c-Src inhibitor 1, #S2075), retinoic acid (RA) (#r2625), and ba lomycin A1 (#B1793) were obtained from Sigma (St. Louis, MO). Antibody for pSer 129 α-syn (#015-25191) was purchased from Wako (Richmond, VA).
Recombinant α-syn monomers and brils were prepared as described previously [24] . Aβ (#RP10017) was purchased from GenScript (Piscataway, NJ). Aβ brils were prepared as described previously [25] . The status of brils was determined by thio avin T binding assay.

Ha T., Choi Y.R., Noh H.R., Cha S., Kim J., & Park S.M. (2021). Age-Related Increase in Caveolin-1 Expression Facilitates Cell-to-Cell Transmission of α-Synuclein in Neurons.

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