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Ligase 3

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Ligase 3 is a DNA repair enzyme that catalyzes the joining of DNA strands with nicks or breaks. It plays a critical role in the non-homologous end joining pathway of DNA double-strand break repair. Ligase 3 functions by forming a covalent phosphodiester bond between the 3' hydroxyl and 5' phosphate termini of DNA.

Automatically generated - may contain errors

Lab products found in correlation

Tubulin, by Merck Group (2 mentions) Anti mouse hrp, by GE Healthcare (2 mentions) Dna pkcs, by Abcam (2 mentions) Rabbit flag, by Merck Group (2 mentions) Brdu a488 3d4, by BD (2 mentions) Ligase 4, by Abcam (2 mentions) Anti rabbit hrp, by GE Healthcare (2 mentions) G box imager, by Syngene (2 mentions)

2 protocols using ligase 3

1

Immunoblotting Protocol for Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunoblotting was performed as described 7 (link) and developed using a Syngene G-Box imager. Antibodies: TRF2 (Karlseder lab), Rabbit FLAG (Sigma-Aldrich - F7425), FLAG (M2, Sigma-Aldrich - F1804), Tubulin (Sigma-Aldrich – T6557), BrdU A488 (3D4, BD Biosciences - 555627), Ku70 (V540, Cell Signalling - 4104), Ku70 (Abcam – ab3114), Ku86 (Cell Signalling - 2753), ATM (Epitomics - 1549-1), Ligase 4 (EPR16531, Abcam - ab193353), DNA-PKcs (Abcam - ab70250), Ligase 3 (BD Biosciences - 611876), Anti-Rabbit HRP (GE Healthcare - NXA931), Anti-Mouse HRP (GE Healthcare - NXA934V).
Arnoult N., Correia A., Ma J., Merlo A., Garcia-Gomez S., Maric M., Tognetti M., Benner C.W., Boulton S.J., Saghatelian A, & Karlseder J. (2017). Regulation of DNA Repair pathway choice in S/G2 by the NHEJ inhibitor CYREN. Nature, 549(7673), 548-552.
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2

Immunoblotting Protocol for Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunoblotting was performed as described 7 (link) and developed using a Syngene G-Box imager. Antibodies: TRF2 (Karlseder lab), Rabbit FLAG (Sigma-Aldrich - F7425), FLAG (M2, Sigma-Aldrich - F1804), Tubulin (Sigma-Aldrich – T6557), BrdU A488 (3D4, BD Biosciences - 555627), Ku70 (V540, Cell Signalling - 4104), Ku70 (Abcam – ab3114), Ku86 (Cell Signalling - 2753), ATM (Epitomics - 1549-1), Ligase 4 (EPR16531, Abcam - ab193353), DNA-PKcs (Abcam - ab70250), Ligase 3 (BD Biosciences - 611876), Anti-Rabbit HRP (GE Healthcare - NXA931), Anti-Mouse HRP (GE Healthcare - NXA934V).
Arnoult N., Correia A., Ma J., Merlo A., Garcia-Gomez S., Maric M., Tognetti M., Benner C.W., Boulton S.J., Saghatelian A, & Karlseder J. (2017). Regulation of DNA Repair pathway choice in S/G2 by the NHEJ inhibitor CYREN. Nature, 549(7673), 548-552.
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