Cells or paraffin-embedded skin biopsies on glass coverslips were washed in PBS, fixed in 4% paraformaldehyde, quenched with 50 mM ammonium chloride, permeabilized with 0.3% Triton X-100 in PBS, saturated with 3% goat serum (Life Technologies), and incubated with primary antibodies at room temperature (Abcam, SMA; Santa Cruz, PDGF-A; Allele Biotechnology, mRFP) for 1 hr, followed by Alexa fluorescein-labeled secondary antibodies (Life Technologies) and Prolong Fade with 4′,6-diamidino-2-phenylindole (DAPI) (Life Technologies). Images were quantified using ImageJ (http://imagej.nih.gov/ij/ ) and Cell Profiler (www.cellprofiler.org ).
Alexa fluorescein labeled secondary antibodies
Manufactured by Thermo Fisher Scientific
Sourced in United States, Cameroon
Alexa fluorescein-labeled secondary antibodies are a type of fluorescent-labeled secondary antibodies used in various immunoassay techniques. They are designed to detect and visualize the presence of primary antibodies bound to target antigens. The fluorescein label provides a means to observe and quantify the targeted analytes.
Automatically generated - may contain errors
Lab products found in correlation
Goat serum, by Thermo Fisher Scientific (2 mentions)
Prolong fade with dapi, by Thermo Fisher Scientific (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
I2 u microscope, by Nikon (1 mentions)
Prolong antifade medium, by Thermo Fisher Scientific (1 mentions)
5 protocols using alexa fluorescein labeled secondary antibodies
1
Immunofluorescent Staining of Skin Biopsies
2
Immunofluorescence Analysis of DNA Damage Markers
3
mRFP Immunofluorescence Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells on glass coverslips were washed in PBS, fixed in 4% paraformaldehyde, quenched with 50 mM glycine, permeabilized with 0.3% Triton X-100 in PBS, saturated with 3% goat serum (Life Technologies), and incubated with mRFP primary antibody (Allele Biotechnology, #5F8) at room temperature for 1 hour, followed by incubation with Alexa fluorescein-labeled secondary antibodies (Life Technologies) for 45 minutes and mounted using Prolong Fade with Dapi (Life Technologies).
4
Immunofluorescence Staining of ZO-1
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells with various indicated treatments were washed twice with PBS, then cells were fixed with 4% paraformaldehyde for 10 min and permeabilized with 0.15% Triton X-100 in PBS for 15 min at room temperatures. Cells were then blocked with 3% BSA for 30 min and incubated with indicated primary antibody against ZO-1 (rabbit monoclonal, 1:2000, CST, USA) overnight at 4 °C, followed by incubation with Alexa fluorescein-labeled secondary antibodies (1:200, Thermo Fisher Scientific, Seoul, South Korea) for 1h and mounted with DAPI (Thermo Fisher Scientific, Seoul, South Korea). Images were captured with a Nikon i2 U microscope (Japan).
5
Immunohistochemical Analysis of Mouse Brain
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Brain tissues from mice sacrificed at the indicated time point were fixed in 4% paraformaldehyde for 24 h and dehydrated with 30 % sucrose prior to OCT freezing. Frozen tissues were cut at 50 μm intervals by a Leica microtome cryostats (CM1850). Sections were permeabilized with 0.3 % Triton X-100 in PBS, saturated with 3 % goat serum (Thermo Fisher Scientific, Seoul, Korea), and incubated with phosphorylated tau, GFAP and Iba-1 antibodies (1:200, Cell Signaling Technology, Danvers, MA) at 4 °C overnight, followed by rinses with PBS and incubation with Alexa fluorescein-labeled secondary antibodies (Thermo Fisher Scientific, Seoul, Korea) for 1 h and mounting using Prolong anti-fade medium (Thermo Fisher Scientific, Seoul, Korea).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!