Multiplex cytokine panel assay

Manufactured by Bio-Rad

The Multiplex cytokine panel assay is a laboratory tool that allows for the simultaneous detection and quantification of multiple cytokines in a single sample. It provides a comprehensive assessment of the cytokine profile in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Bioplex bead array technique, by Bio-Rad (3 mentions) Luminex bead array technique, by Bio-Rad (2 mentions) Bio plex bead array, by Bio-Rad (1 mentions)

Close spelling variants of this product

Cytokine multiplex assay Multiplex panel Multiplex assay

7 protocols using multiplex cytokine panel assay

Cytokine and MMP2 Quantification Protocol

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Quantification of cytokine expression and MMP2 activity in murine aortic tissue and culture supernatants was performed using the Luminex Bead Array technique using a multiplex cytokine panel assay (Bio-Rad Laboratories, Hercules, CA), as previously described (13 (link), 30 (link)).

Ladd Z., Su G., Hartman J., Lu G., Hensley S., Upchurch GR J.r, & Sharma A.K. (2023). Pharmacologic inhibition by spironolactone attenuates experimental abdominal aortic aneurysms. Frontiers in Cardiovascular Medicine, 10, 1101389.

+ Open protocol

+ Expand

Multiplex Cytokine Profiling in Lungs

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Using separate groups of animals, proinflammatory cytokines were measured in whole lung lysates using a multiplex cytokine panel assay (Bio-Rad Laboratories, Hercules, CA) as described previously (5 (link), 21 (link)).

Stone M.L., Sharma A.K., Mas V.R., Gehrau R.C., Mulloy D.P., Zhao Y., Lau C.L., Kron I.L, & Laubach V.E. (2015). Ex vivo Perfusion with Adenosine A2A Receptor Agonist Enhances Rehabilitation of Murine Donor Lungs after Circulatory Death. Transplantation, 99(12), 2494-2503.

+ Open protocol

+ Expand

Multiplex cytokine quantification in BAL

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cytokine concentrations in BAL fluid were quantified using the Bioplex Bead Array technique and a multiplex cytokine panel assay (Bio-Rad Laboratories, Hercules, CA) as previously described [3 (link)].

Stone M.L., Zhao Y., Robert Smith J., Weiss M.L., Kron I.L., Laubach V.E, & Sharma A.K. (2017). Mesenchymal stromal cell-derived extracellular vesicles attenuate lung ischemia-reperfusion injury and enhance reconditioning of donor lungs after circulatory death. Respiratory Research, 18, 212.

+ Open protocol

+ Expand

Multiplexed Cytokine Profiling in Rat Aortic Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cytokine content in rat aortic tissue homogenates was quantified using the Bio-Plex bead array technique using a multiplex cytokine panel assay (Bio-Rad Laboratories, Hercules, CA). Tissue inhibitor of metalloproteinase 1 (TIMP-1) concentrations were determined in terms of mean pixel intensity using a Proteome Profiler Array Rat Cytokine Array Panel A (R&D Systems, Inc, Minneapolis, MN), and array data were developed on x-ray film and quantified using Image Lab software (Version 3.0; Bio-Rad Laboratories, Hercules, CA). Western blot analyses for uPA, tPA, and PAI-1 protein concentrations were performed in a standard fashion.

English S.J., Piert M.R., Diaz J.A., Gordon D., Ghosh A., D'Alecy L.G., Whitesall S.E., Sharma A.K., DeRoo E.P., Watt T., Su G., Henke P.K., Eliason J.L., Ailawadi G, & Upchurch GR J.r. (2015). Increased 18F-FDG Uptake Is Predictive of Rupture in a Novel Rat Abdominal Aortic Aneurysm Rupture Model. Annals of surgery, 261(2), 395-404.

+ Open protocol

+ Expand

Cytokine Quantification in Murine Aortic Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cytokine expression in murine aortic tissue homogenates and cell culture supernatants was quantified using the Luminex Bead Array technique using a multiplex cytokine-panel assay (Bio-Rad Laboratories, Hercules, CA).

Filiberto A.C., Spinosa M.D., Elder C.T., Su G., Leroy V., Ladd Z., Lu G., Mehaffey J.H., Salmon M.D., Hawkins R.B., Ravichandran K.S., Isakson B.E., Upchurch GR J.r, & Sharma A.K. (2022). Endothelial pannexin-1 channels modulate macrophage and smooth muscle cell activation in abdominal aortic aneurysm formation. Nature Communications, 13, 1521.

+ Open protocol

+ Expand

Cytokine Quantification in Murine Aortic Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Shannon A.H., Elder C.T., Lu G., Su G., Mast A., Salmon M.D., Montgomery W.G., Spinosa M.D., Upchurch GR J.r, & Sharma A.K. (2020). Pharmacologic Inhibition of Transient Receptor Channel Vanilloid 4 Attenuates Abdominal Aortic Aneurysm Formation. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(7), 9787-9801.

+ Open protocol

+ Expand

Quantification of Cytokines and HMGB1

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cytokine content in murine aortic tissue homogenates and cell culture supernatants was quantified using the Bioplex Bead Array technique using a multiplex cytokine panel assay (Bio-Rad Laboratories, Hercules, CA) per the manufacturer's instructions. HMGB1 was measured in cell culture supernatants using an ELISA kit per the manufacturer's instructions (IBL International, Hamburg, Germany).

Filiberto A.C., Ladd Z., Leroy V., Su G., Elder C.T., Pruitt E.Y., Hensley S.E., Lu G., Hartman J.B., Zarrinpar A., Sharma A.K, & Upchurch GR J.r. (2022). Resolution of inflammation via RvD1/FPR2 signaling mitigates Nox2 activation and ferroptosis of macrophages in experimental abdominal aortic aneurysms. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 36(11).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!