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Accucore c18

Manufactured by Thermo Fisher Scientific
Sourced in United States, Hungary

Accucore C18 is a reversed-phase high-performance liquid chromatography (HPLC) column. It features a 2.6 μm core-shell particle technology that provides high efficiency and resolution. The column is designed for a variety of analytical applications.

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50 protocols using accucore c18

1

LC-MS/MS Analysis of S. caseolaris Extracts

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The extracted leaves paste of S. caseolaris from the ethanol solvent was dissolved into 1 mL methanol. The mixture was injected into the LC column at a flow rate of 0.2 mL/min at 30 °C. The system of LC-MS/MS was UHPLC Vanquish Tandem Q Exactive Plus Orbitrap HRMS from Thermo Fisher Scientific, Waltham, MA, USA. The ionisation mode on the MS system used was negative ionisation. The scan mode used was a full scan from 100–500 m/z. The UPLC column used was Accucore C18, 100 × 2.1 mm, 1.5 µm (Thermo Fisher Scientific, Waltham, MA, USA). The eluent used was H2O + 0.1% Formic acid (A) and acetonitrile + 0.1% formic acid (B). The results of the analysis were displayed using a mass chromatogram.
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2

Quantitative Analysis of Polyphenols in Shoumei Tea

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Ten milligrams of standard product (dried at 25°C for 24 h, Shanghai Yuanye Bio-Technology Co., Ltd., Shanghai, China) was accurately weighed to the nearest 0.01 mg. The standard was placed into a 10 mL volumetric flask, dissolved, and fixed with an appropriate amount of methanol. At the same time, 12.5 mg of polyphenol extract was accurately weighed, placed into a 25 mL volumetric flask, dissolved, and fixed with an appropriate amount of methanol. The flasks were shaken well to obtain 0.5 mg/mL test solutions. The polyphenolic components in the Shoumei tea samples were detected by chromatography under the following conditions using a diode array HPLC detector (Thermo Scientific Accucore C18 (2.6 μm, 4.6 mm × 150 mm)): mobile phase A = 0.1%formic acid aqueous solution; mobile phase B = acetonitrile; column temperature = 30°C; flow rate = 0.5 mL/min; injection volume = 10 μL; and detection wavelength = 359 nm (UltiMate 3000; Thermo Fisher Scientific, Waltham, MA, USA). The gradient elution conditions of the mobile phase are listed in Table 1.
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3

Characterization of PDES-GO Nanoparticles

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The morphology of PDES-GO was examined by scanning electron microscopy (SEM, Phenom-World BV, Eindhoven, The Netherlands). Fourier-transform infrared (FTIR) spectroscopy was performed using a Thermo Scientific Nicolet iS10 FTIR spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). A Thermo UltiMate3000 DGLC HPLC system (Thermo Fisher Scientific, Waltham, MA, USA) with a Chromeleon 7.2 workstation, diode array detector, and chromatographic column (Accucore C18, 100 mm × 4.6 mm, 2.6 μm) was employed for the analysis of I3C. The mobile phase consisted of acetonitrile-water (40:60, V/V) at a flow rate of 1.0 mL/min. The analysis wavelength of the detector was 218 nm, and the injection volume was 20 μL.
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4

HPLC Separation of Compound Mixture

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The chromatographic separation was made with an Ultimate 3000 HPLC system (Thermo Fischer Scientific) equipped with a column Accucore C18 (100 × 4.6 mm, 2.6 µm). The mobile phase consisted of: (A) water with ammonium acetate (50 mM) adjusted at pH 4.4 with acetic acid, and (B) acetonitrile 100%. The gradient used is presented in Table 1. To separate the compounds in the peak, the gradient began with a high percentage of the aqueous phase and a low flow; as time passed, the percentage of organic proportion increased along with the flow of the mobile phase.
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5

UHPLC Analysis of Short-Chain Fatty Acids

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Following a previous study [24 (link)], analysis of the SCFAs was performed using ultra-high performance liquid chromatography (UHPLC). There was no need for sample pre-treatment prior to injection. SCFA standards of acetic, butyric, lactic, propionic, and succinic acids (all from Merck; Darmstadt, Germany), prepared in the mobile phase at concentrations ranging from 5 to 10,000 ppm, were quickly created. The mobile phase was supplied at a flow rate of 0.250 mL/min and consisted of a mixture of two solutions. The mobile phase consisted of aqueous acetonitrile (1%) and ultrapure water (99%), both acidified with 1% formic acid. After fermentation, 1 mL of the supernatant was centrifuged, filtered through a 0.22 µm nylon filter, and then transferred to a vial for UHPLC analysis. The UV–Vis photodiode array detector (PDA) was set at 210 nm, and the column was a reversed-phase Accucore™ C18 (ThermoFisher Scientific, Waltham, MA, USA) with a particle size of 2.6 µm and length of 150 mm set at 35 °C. The analysis was performed twice, and the information shown represents the mean values of the millimolar (mM) concentration of each SCFA.
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6

Chromatographic Separation of Compounds

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Chromatographic separation was accomplished with a Dionex Ultimate 3000RS UHPLC instrument, equipped with Thermo Accucore C18 (100 mm × 2.1 mm i.d., 2.6 μm) analytical column for separation of compounds. Water (A) and methanol (B) containing 0.1% formic acid were employed as mobile phases, respectively. The total run time was 70 min; the elution profile and all exact analytical conditions have been published [67 (link)].
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7

Chromatographic Separation and Mass Spectrometry

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Chromatographic separation was accomplished with a Dionex Ultimate 3000RS UHPLC instrument, equipped with a Thermo Accucore C18 (100 mm × 2.1 mm i. d., 2.6 μm) analytical column for the separation of compounds. Water (A) and methanol (B) containing 0.1% formic acid were employed as mobile phases, respectively. The total run time was 70 min for the elution profile. Mass spectrum analysis was carried out using a Thermo Q-Exactive Orbitrap mass spectrometer (Thermo Scientific, Waltham, MA, USA) equipped with an electrospray ionisation probe interface in positive and negative-ion mode. All detailed analytical conditions have been published [62 (link)].
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8

HPLC-DAD Analysis of Coffee Compounds

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Instrumental analysis of the CGAs and caffeine was performed using a high-performance liquid chromatography coupled with diode array detector (HPLC-DAD) system (Dionex, UltiMate 3000, Sunnyvale, CA, USA) with an Accucore C18 (150 mm × 4.6 mm, 2.6 μm, Thermo Scientific, MA, USA). The mobile phase was composed of eluent A (20 mM KH2PO4 buffer containing 0.1% phosphoric acid) and eluent B (acetonitrile containing 0.1% phosphoric acid). The gradient mode was initially set at an A/B ratio of 97:3 from 0 to 5 min, the eluent was increased to 93:7 from 5 to 15 min, eluent B was slightly increased to 92:8 from 15 to 25 min, then eluent B was decreased to 75:25 from 25 to 35 min, and finally the eluent was returned to 97:3 from 35 to 45 min for column equilibration and system washing. The gradient program was carefully conducted to separate the 7 isomers of CGAs and caffeine (Fig. 1). The flow rate and injection volume were 1 mL/min and 10 μL, respectively. Detection wavelengths of 324 nm and 272 nm were used for the analysis of CGAs and caffeine, respectively [35] .

Identification of 3-CQA, 4-CQA, 5-CQA, 3- FQA, 3,4-diCQA, 3,5-diCQA, 4,5-diCQA, and caffeine from chromatograms of the roasted and ground coffee samples.

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9

LC-MS Analysis of Compounds

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LC–MS analysis was performed on Acquity Ultra Performance Liquid Chromatography equipped with a XEVO- TQD interfaced via an ESI source (Waters Co., Milford, CT, USA). The compounds were separated on a Thermo Scientific™ ACCUCORE C-18, 150 × 2.1 mm, 2.6 µm reverse-phase column at a constant flow rate of 0.25 mL/min. An applied column and the auto-sampler temperatures were 35 ± 5 °C and 25 ± 5 °C, respectively. The mobile phase consisted of three solvents. Acetonitrile (A) and 0.1% formic acid buffer was prepared in 95:5 v/v, and water/ acetonitrile (B) using a multi-step gradient was applied, as shown in Table 6, with a sample injection volume of 2 μL.
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10

HPLC Analysis of Tamoxifen and Metabolite

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HPLC analysis of tamoxifen and tamoxifen N-oxide was performed on a Vanquish Flex HPLC system (Thermo Fisher Scientific) using an Accucore C18 reversed-phase column (150 × 3 mm, 2.6 μm; Thermo Fisher Scientific). Tamoxifen and tamoxifen N-oxide were separated by isocratic elution of 55% Milli-Q water (+0.1% trifluoroacetic acid) and 45% acetonitrile (+0.1% trifluoroacetic acid) using a column temperature of 68 °C and a flow rate of 1.1 mL min−1. The detection wavelength was set to 276 nm. Retention times of the commercially available standards of tamoxifen and tamoxifen N-oxide were 4.0 and 4.8 min, respectively.37 (link)
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