In order to evaluate the adhesion potential of MDA-MB-231 breast cancer cells, the following adhesion protocol was conducted, as it was described by previous works [39 (link),40 (link)]. Briefly, 96-well plate was coated with collagen type I solution (40 μg/mL) and kept at 4 °C. After 12 h, the solution was removed, and the plate was air-dried; 3% BSA in PBS solution was added in each well, for 30 min, to block non-specific adsorption. Then the solution was removed, and the plate was washed with PBS and air-dried. Cells treated for 24 h prior to the adhesion assay were detached with PBS-EDTA 1× and resuspended in serum-free medium with 0.1% BSA. and seeded at a density of 2 × 104 cells/well. Cells were incubated for 30 min, to allow adhesion to the surface. Non-adherent cells were removed with serum free medium, and then cells were incubated with medium supplemented with 10% FBS for 2–3 h for recovery. After the incubation period, Premix WST-1 (water-soluble tetra-zolium salt) Cell Proliferation Assay System (Takara Bio Inc., Göteborg, Sweden) was added at a ratio 1:10, and the absorbance at 450 nm was measured (reference wavelength at 650 nm).
Cell proliferation assay system
Manufactured by Takara Bio
Sourced in Japan, Sweden
The Cell Proliferation Assay System is a tool designed to measure and analyze the growth and proliferation of cells in a laboratory setting. It provides quantitative data on cell population dynamics, enabling researchers to study various cellular processes and responses.
Automatically generated - may contain errors
Lab products found in correlation
Premix wst 1, by Takara Bio (5 mentions)
Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Silver stainreagent, by Cosmo Bio (1 mentions)
Gefitinib, by Cell Signaling Technology (1 mentions)
Fitc dextran 70 kda, by Merck Group (1 mentions)
Hoechst 33342, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
5 protocols using cell proliferation assay system
1
Adhesion Potential of MDA-MB-231 Breast Cancer Cells
2
Cell Proliferation Assay with WST-1
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Parental and transfected breast cancer cells were seeded in the presence of FBS into 96-well plates at a density of 5,000 cells/well and then the cells were incubated in serum-free culture medium. Premix WST-1 (water-soluble tetrazolium salt) Cell Proliferation Assay System (Takara Bio Inc., Japan) was added after 24 hours at a ratio 1:10. The assay is based on the reduction of WST-1 by viable cells, producing a soluble formazan salt absorbing at 450 nm (reference wavelength at 650 nm).
3
Collagen-Induced Proliferation of MSCs
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Wharton’s jelly MSCs and DPSCs at passage five were seeded in 48-well plates containing type II collagen at a density of 3,200 cells/well and then the cells were incubated in DMEM supplemented with 2% FBS for 3, 7, 10, 14 and 24 days. Medium was replaced every two days following one wash with PBS 1X. Premix WST-1 (water-soluble tetrazolium salt) Cell Proliferation Assay System (Takara Bio Inc., Japan) was added after the treatment period at a ratio 1:10 for 1-3 h depending on cell density. The assay is based on the reduction of WST-1 by viable cells, producing a soluble formazan salt absorbing at 450 nm (reference wavelength at 650 nm). Following incubation, the optical density of each well was measured at 450 nm with a TECAN spectrophotometer.
4
Breast Cancer Cell Adhesion Assay
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In order to evaluate the adhesion potential of breast cancer cells, the following adhesion protocol was performed, as previously described (25 (link)). Briefly, 96-well plate was coated with collagen type I (40 μg/ml) and kept at 4°C. After 12 hours, the solution was removed, and the plate was air-dried; 3% BSA in PBS solution was added in each well, for 30 minutes, to block non-specific adsorption. Then the solution was removed, and the plate was washed with PBS and air-dried. Cells treated for 24 hours prior to the adhesion assay were detached with PBS-EDTA 1x and resuspended in serum-free medium with 0.1% BSA. and seeded at a density of 2x104 cells/well. Cells were incubated for 30 min, to allow adhesion to the surface. Non-adherent cells were removed with serum free medium, and then cells were incubated with medium supplemented with 10% FBS for 2 hours for recovery. Premix WST-1 (water-soluble tetra-zolium salt) Cell Proliferation Assay System (Takara Bio Inc., Göteborg, Sweden) was then added at a ratio 1:10, and the absorbance at 450 nm was measured (reference wavelength at 650 nm).
5
Evaluating Liposomes for Cancer Treatment
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