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2 protocols using xanthotoxol

1

Analysis of Phytochemicals in Herbal Extracts

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Deionized water was used for HPLC-Q-TOF-MS and HPLC-Q-TOF-MS/MS analysis and was purified using a Milli-Q system (Merck Millipore, Billerica, MA, USA). HPLC-grade acetonitrile and formic acid were purchased from Merck (Darmstadt, Germany) and ROE (Newark, New Castle, USA), respectively. Methanol (AR) was purchased from the National Institutes for Food and Drug Control (Beijing, China). The seventeen reference substances (seven flavonol glycosides, three flavonols, two polymethoxyflavonoids, three coumarins, one alkaloid, and one limonin), including synephrine (15), neoeriocitrin (36), poncirin (39), eriocitrin (40), naringin (42), naringenin (43), narirutin (44), neohesperidin (51), hesperidin (56), apigenin (59), hesperitin (61), xanthotoxol (71), scopoletin (76), auraptene (80), limonin (79), nobiletin (85), and tangeretin (87) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China).
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2

Analysis of Compounds in A. dahuricae

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A total of 14 batches of A. dahuricae from different regions were widely collected throughout China and Dr. Luping Qin (Naval Medical University, Shanghai, China) further confirmed their authenticity. Reference compounds, xanthotoxol, xanthotoxin, bergapten, imperatorin and isoimperatorin were provided by Shanghai yuanye Bio-Technology Co., Ltd. Reference compounds, prangenin hydrate, hydrate oxypeucedanin, oxypeucedanin and phellopterin were isolated and purified by our laboratory from A. dahuricae with their purities over 95% at HPLC level. Internal standard (IS), estazolam was purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Methanol (Merck company, Darmstadt, Germany) was HPLC grade. Ethanol and formic acid (China Medicine Group Shanghai Chemical Reagent Corporation, Shanghai, China) was analytical grade. Deionized water (18 MΩ, Milli-Q, Millipore, Bedford, MA, USA) was used in the study.
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