The Ph.D.™ Phage Display Kit, BSA, BspQI, T4 Ligase, Q5 High Fidelity polymerase, dNTPs, and BL21(DE3) E. coli were purchased from New England Biolabs. Antibiotics were purchased from GoldBio. Na2SeO3 and HNaSeO3 were purchased from Alfa Aesar. NADPH was purchased from BioVision and Coomassie Plus Bradford Reagent from Thermo Scientific. GeneJet Plasmid Miniprep Kit (Cat# K0503) and PCR Cleanup Kit (Cat# K0702) were purchased from ThermoFisher Scientific.
Na2seo3
Manufactured by Thermo Fisher Scientific
Sourced in Germany
Na2SeO3 is a chemical compound that is used as a laboratory reagent. It is a sodium salt of selenious acid. Na2SeO3 is a white crystalline solid that is soluble in water.
Automatically generated - may contain errors
Lab products found in correlation
Genejet plasmid miniprep kit, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by New England Biolabs (1 mentions)
Bspqi, by New England Biolabs (1 mentions)
Nadph, by Abcam (1 mentions)
Coomassie plus bradford reagent, by Thermo Fisher Scientific (1 mentions)
Pcr cleanup kit, by Thermo Fisher Scientific (1 mentions)
T4 ligase, by New England Biolabs (1 mentions)
Q5 high fidelity polymerase, by New England Biolabs (1 mentions)
E coli bl21 de3, by New England Biolabs (1 mentions)
Dntps, by New England Biolabs (1 mentions)
Kanamycin, by Teknova (1 mentions)
Lb medium, by Teknova (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
3 protocols using na2seo3
1
Phage Display Kit Protocol
2
Heterotrophic Cultivation of Chlorella vulgaris
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Green freshwater microalga C. vulgaris G-120 (registered as C. vulgaris BEIJ., 1996/H 14, CCALA 30,001, Culture Collection of Autotrophic Organisms, Institute of Botany, Třeboň, Czech Republic) was cultivated heterotrophically for 4 days in conical flasks (300 mL starting volume). The flasks were placed on an orbital shaker (120 rpm) and grown in the dark at 25 °C in a batch mode. The initial biomass density was about 2 g dry weight (DW) per liter. The cultivation medium was described by Mylenko et al. [6 (link)].
Growth of the cultures was monitored by optical density at 750 nm, which is directly proportional to the biomass DW according to the empiric formula: DW [g/L] = OD750/2. Based on the optical density measurements, Se was supplemented to the culture twice a day in the form of Na2SeO3 (Alfa Aesar, Karlsruhe, Germany) in concentrations of 0.5, 1, 4, 8, and 16 mg Se/g DW. The pH of the medium was 7.5.
Growth of the cultures was monitored by optical density at 750 nm, which is directly proportional to the biomass DW according to the empiric formula: DW [g/L] = OD750/2. Based on the optical density measurements, Se was supplemented to the culture twice a day in the form of Na2SeO3 (Alfa Aesar, Karlsruhe, Germany) in concentrations of 0.5, 1, 4, 8, and 16 mg Se/g DW. The pH of the medium was 7.5.
3
Visualizing Metalloid Reductase Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A
volume of 3 mL of BL21 cells containing either a metalloid reductase
gene or GFP reporter gene was grown separately in 10 mL culture tubes
overnight containing LB medium (Teknova) supplemented with Kanamycin
at 25 μg/mL. The following morning, the culture was added to
a 125 mL Erlenmeyer flask containing LB medium supplemented with Kanamycin
(25 μg/mL). The cells were grown for 2.5 h, and 100 mM Na2SeO3 (Alfa Aesar, 98+%) was added to reach a final
concentration of 5 mM. The cells were collected by centrifuging for
20 min at 4000 rpm and 4 °C after 3 h of growth with selenite.
The cells were washed with 20 mM Tris (pH 7.4) (Fischer) three times
followed by resuspension in 1 mL of fixing solution (2% glutaraldehyde
(25% Sigma-Aldrich) and 2.5% formaldehyde); the fixing solution was
allowed to react for 12 h at 4 °C. The fixing solution was centrifuged
and the pellet was washed five times in 20 mM Tris (pH 7.4). The cells
were resuspended in 1 mL of 20 mM Tris (pH 7.4). The aliquots (4 μL)
were mounted on 400 mesh Cu grids with 50 nm C coating and washed
two times with H2O. The dry-mounted cells on transmission
electron microscopy grids were loaded onto a STEM holder. The STEM
images were taken with a JEOL JSM-6500-F scanning electron microscope
at an accelerating voltage of 15 kV.
volume of 3 mL of BL21 cells containing either a metalloid reductase
gene or GFP reporter gene was grown separately in 10 mL culture tubes
overnight containing LB medium (Teknova) supplemented with Kanamycin
at 25 μg/mL. The following morning, the culture was added to
a 125 mL Erlenmeyer flask containing LB medium supplemented with Kanamycin
(25 μg/mL). The cells were grown for 2.5 h, and 100 mM Na2SeO3 (Alfa Aesar, 98+%) was added to reach a final
concentration of 5 mM. The cells were collected by centrifuging for
20 min at 4000 rpm and 4 °C after 3 h of growth with selenite.
The cells were washed with 20 mM Tris (pH 7.4) (Fischer) three times
followed by resuspension in 1 mL of fixing solution (2% glutaraldehyde
(25% Sigma-Aldrich) and 2.5% formaldehyde); the fixing solution was
allowed to react for 12 h at 4 °C. The fixing solution was centrifuged
and the pellet was washed five times in 20 mM Tris (pH 7.4). The cells
were resuspended in 1 mL of 20 mM Tris (pH 7.4). The aliquots (4 μL)
were mounted on 400 mesh Cu grids with 50 nm C coating and washed
two times with H2O. The dry-mounted cells on transmission
electron microscopy grids were loaded onto a STEM holder. The STEM
images were taken with a JEOL JSM-6500-F scanning electron microscope
at an accelerating voltage of 15 kV.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!