To check the influence of miR‐203a‐3p mimic/inhibitor and siMAP3K1 on the process of the cell cycle of TPC‐1 and KTC‐1 cells, cell cycle evaluation was carried out using the PI/RNase staining reagent (Becton), and were detected with a flow cytometer (Beckman Coulter) as previously described.18
Pi rnase staining reagent
Manufactured by BD
Sourced in United States
The PI/RNase staining reagent is a laboratory product used for DNA staining and analysis. It contains propidium iodide (PI) and RNase. Propidium iodide is a fluorescent dye that binds to DNA, allowing for the detection and quantification of DNA content in cells. RNase is included to remove any interfering RNA, ensuring that the measured DNA content is from the cell's nucleus. This reagent is commonly used in flow cytometry and other applications requiring DNA analysis.
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Lab products found in correlation
2 protocols using pi rnase staining reagent
1
Analyzing Cell Cycle Dynamics in Thyroid Cancer
2
Cell Cycle Analysis of HCT-116 Cells
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For cell cycle analysis, 1 × 105 HCT-116 cells were seeded in 12-well plates. On the second day, either OPT-A or DMSO (vehicle) was administrated. Treated cells continued to be cultured for 48 h. Then, all adherent cells were trypsinized, collected and fixed in 80% ethanol for 2 h at −20 °C. After being treated with 0.25% Triton X-100 for 5 min, the cells were resuspended in 50 µL of PI/RNase staining reagent (Becton Dickinson, San Diego, CA, USA), incubated in the dark for 20 min at room temperature, and finally counted with a FACS Canto flow cytometer [18 (link)]. At least 10,000 cells were read for each measurement.
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