Anti his tag specific primary antibody

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analyses were performed as described by Kunze et al. (1998) (link). The antibodies used for Western blot analysis were anti-His-tag specific primary antibody produced in rabbit (Sigma-Aldrich, St. Louis, MO, United States) and secondary antibody anti-mouse IgG alkaline phosphatase conjugate produced in goat (Sigma-Aldrich, St. Louis, MO, United States). The staining procedure was done by membrane incubation with nitro-blue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) substrate (Roche Diagnostics, Rotkreuz, Switzerland).
The dye-binding method of Bradford was used for protein quantification (Bio-Rad, Hercules, CA, United States) using bovine serum albumin as the standard (Bradford, 1976 (link)).

SDS-PAGE and western analysis were performed as described by Kunze et al. [37 (link)]. Western blots were treated with an anti-His-tag specific primary antibody produced in mice (Sigma-Aldrich, Germany) and a rabbit anti-mouse IgG alkaline phosphatase conjugate (Sigma-Aldrich, USA), and subsequently stained by incubation with NBT/BCIP substrate (Roche Diagnostics, Switzerland).
The dye-binding method of Bradford [39 (link)] was used for protein quantification (BIO-RAD, USA), using bovine serum albumin as the standard.

SDS-PAGE and Western blot analysis were performed as described by Kunze et al. (1998 (link)). The antibodies used for Western blot analysis were anti-His-tag specific primary antibody produced in rabbit (Sigma-Aldrich, USA) and secondary antibody anti-mouse IgG alkaline phosphatase conjugate produced in goat (Sigma-Aldrich, USA). The staining procedure was done by membrane incubation with NBT/BCIP substrate (Roche Diagnostics, Switzerland).
The dye-binding method of Bradford was used for protein quantification (BIO-RAD, USA), using bovine serum albumin as the standard (Bradford, 1976 (link)).