Anti centrin

Manufactured by Merck Group

Anti-centrin is a laboratory equipment product designed for the detection and visualization of centrin proteins in cellular samples. Centrin proteins play a crucial role in the organization and function of the centrosome, a key cellular structure involved in cell division and motility. The anti-centrin product provides researchers with a tool to study the distribution and dynamics of centrin proteins within cells, enabling a better understanding of centrosome biology and related cellular processes.

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Lab products found in correlation

Anti β actin, by Santa Cruz Biotechnology (1 mentions) Anti mouse igg hrp, by Promega (1 mentions) Anti mouse igg alexafluor 488, by Thermo Fisher Scientific (1 mentions) Anti γ tubulin, by Merck Group (1 mentions) Anti rabbit igg alexafluor 555, by Thermo Fisher Scientific (1 mentions) Anti rabbit igg hrp, by Promega (1 mentions) Anti p15, by Santa Cruz Biotechnology (1 mentions) Anti hsp90, by BD (1 mentions) Alexa fluor 488 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 donkey anti goat igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 555 donkey anti mouse igg, by Thermo Fisher Scientific (1 mentions) Anti cep164, by Santa Cruz Biotechnology (1 mentions) Anti γ tubulin, by Abcam (1 mentions) Anti α tubulin, by Merck Group (1 mentions) Anti usp9x, by Fortis Life Sciences (1 mentions) Anti myc, by Santa Cruz Biotechnology (1 mentions) Anti cep290, by Fortis Life Sciences (1 mentions) Anti ub, by Agilent Technologies (1 mentions) Anti k48 ub, by Merck Group (1 mentions) Anti k 63 ub, by Abcam (1 mentions)

4 protocols using anti centrin

Antibody Panel for Centrosome Proteins

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The following primary antibodies were used in this study: anti-CEP128 [Abcam, ab118797; immunofluorescence (IF) 1:500, western blotting (WB) 1:1000], anti-γ-tubulin (Merck, GTU88; IF 1:1000), anti-p15 (Santa Cruz Biotechnology, sc-271791; WB 1:1000), anti-centrin (Millipore, A302-479A; IF 1:1000), anti-KLC1 (Abcam, ab187179; IF 1:500), anti-HSP90 (BD Biosciences, 610419; WB 1:1000) and anti-β-actin (Santa Cruz Biotechnology, sc-47778; WB 1:1000). The following secondary antibodies were used: anti-mouse IgG Alexa Fluor 488 (Molecular Probes, 1:1000), anti-rabbit IgG Alexa Fluor 555 (Molecular Probes, 1:1000), anti-mouse IgG HRP (Promega, WB 1:10,000) and anti-rabbit IgG HRP (Promega, WB 1:10,000).

Komori T., Hata S., Mabuchi A., Genova M., Harada T., Fukuyama M., Chinen T, & Kitagawa D. (2023). A CRISPR-del-based pipeline for complete gene knockout in human diploid cells. Journal of Cell Science, 136(6), jcs260000.

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Immunofluorescence of Centrosome Proteins

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Primary antibodies: anti-Centrin (Millipore, 04–1624), anti-γ-tubulin (Abcam, ab11321), anti-α-tubulin (Sigma, T5168), anti-Cep164 (Santa Cruz, sc-240226). Secondary antibodies for immunofluorescence microscopy: Donkey anti-Rabbit IgG Alexa Fluor 488 (Invitrogen, A21206), Donkey anti-Mouse IgG Alexa Fluor 555 (Invitrogen, A31570), Goat anti-Rat IgG Alexa Fluor 564 (Invitrogen, A11081), Donkey anti-Goat IgG Alexa Fluor 488 (Invitrogen, A21202).

Wang K., Muñoz K.J., Tan M, & Sütterlin C. (2021). Chlamydia and HPV induce centrosome amplification in the host cell through additive mechanisms. Cellular microbiology.

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Antibodies for Ciliopathy Protein Analysis

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Antibodies used in this study included anti-HA, anti-NPHP5, anti-Myc, anti-MARCH7, anti-γ tubulin-FITC (Santa Cruz); anti-Flag, anti-GFP, anti-β actin, (Sigma); anti-CP110, anti-USP9X, anti-CEP290 (Bethyl Laboratories); anti-BBS11 (Thermo Fisher), anti-centrin, anti-K48 Ub (Millipore); anti-K63 Ub (Abcam); anti-Ub (Dako); anti-IFT88 (Proteintech); anti-glutamylated tubulin GT335, anti-acetylated tubulin and anti-Ki67 (Invitrogen). Two additional anti-NPHP5 antibodies (IRCM1 and IRCM2) were also used[25 (link)].

Das A., Qian J, & Tsang W.Y. (2017). USP9X counteracts differential ubiquitination of NPHP5 by MARCH7 and BBS11 to regulate ciliogenesis. PLoS Genetics, 13(5), e1006791.

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Antibody Generation and Characterization for ITPKC

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Affinity purified anti-mouse ITPKC polyclonal antibodies named GS2 and GS4 were made by immunizing rabbits with peptide LPERDNKPRVDNLRC and QPGSDGFSS KDTESC, respectively (GenScript). Affinity purified anti-mouse ITPKC antibody named Ald was produced by immunizing rabbits with three peptides: RGGRRRQPGLQRPGPGAG, KPRQNKELDGSNLQTHPRRN-C and SQTDDSLKGPSTQTAC (Aldevron). Those peptides were chosen in the sequence of mouse ITPKC (Pouillon et al., 2003) . Specificity of detection was confirmed by Western blot and immunofluorescence analysis using anti-ITPKC antibodies preincubated or not with their corresponding immunizing peptides.
Anti-ezrin antibody was obtained from ThermoFisher Scientific. Anti-centrin was purchased from Millipore. Antiacetylated tubulin, phalloidin-TRICT and anti-gamma tubulin were obtained from SigmaeAldrich. Rabbit IgGs and mouse IgGs were from Santa Cruz Biotechnologies. Anti-rabbit IgGs Atto 488 and Atto 594-conjugated secondary antibodies were obtained from SigmaeAldrich. Horse-radish peroxidase (HRP)-conjugated secondary antibodies were obtained from Santa Cruz Biotechnologies.

Scoumanne A., Molina-Ortiz P., Monteyne D., Perez-Morga D., Erneux C, & Schurmans S. (2016). Specific expression and function of inositol 1,4,5-trisphosphate 3-kinase C (ITPKC) in wild type and knock-out mice. Advances in biological regulation, 62.

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