GTS-21, α-bungarotoxin, and antibodies against Bcl2 and PGC-1α were purchased from Abcam (Cambridge, UK). LPS (Escherichia coli serotype 055:B5), methyllycaconitine, and antibodies against β-actin and BDNF were purchased from Sigma-Aldrich (St. Louis, MO, USA). MPTP was obtained from Tokyo Chemical Industry Co. (Tokyo, Japan). Antibodies against Iba-1 were purchased from Wako (Osaka, Japan). Antibodies against phospho-/total forms of AMPK, Akt, MAP kinases, CREB, and antibodies for IL-6, TGF-β, NQO1, PPAR-γ, and TH were obtained from Cell Signaling Technology (Beverley, CA, USA). While antibodies against phospho-p47phox were provided by Assaybiotech (Sunnyvale, CA, USA), those against TNF-α, Nrf2, HO-1, catalase, lamin A, and COX-2 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against iNOS and IL-1β were purchased from BD Biosciences (San Jose, CA, USA), and an antibody for 4-hydroxy-2E-nonenal (HNE) was purchased from Alpha Diagnostic International (San Antonio, TX, USA).
Antibodies against bcl 2
Manufactured by Abcam
Sourced in United States
Antibodies against Bcl-2 are laboratory reagents used to detect and study the Bcl-2 protein, which plays a role in regulating cell survival and apoptosis. These antibodies can be used in various immunoassay techniques, such as Western blotting and immunohistochemistry, to identify and quantify Bcl-2 expression in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
P akt, by Cell Signaling Technology (3 mentions)
β actin, by Proteintech (2 mentions)
Antibodies against cleaved caspase 3, by Proteintech (2 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (2 mentions)
Antibodies against β actin, by Merck Group (2 mentions)
Antibodies against iba1, by Fujifilm (1 mentions)
Gts 21, by Abcam (1 mentions)
α bungarotoxin, by Abcam (1 mentions)
Pparγ, by Cell Signaling Technology (1 mentions)
Cisplatin, by Merck Group (1 mentions)
Anti cleaved caspase 9, by Cell Signaling Technology (1 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Anti p53, by Cell Signaling Technology (1 mentions)
Anti cytochrome c cyt c, by Cell Signaling Technology (1 mentions)
Anti phosphorylated p53 p p53, by Cell Signaling Technology (1 mentions)
Anti poly adp ribose polymerase, by Cell Signaling Technology (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
N acetyl l cysteine, by Beyotime (1 mentions)
β tubulin, by ABclonal (1 mentions)
Ly294002, by Cell Signaling Technology (1 mentions)
6 protocols using antibodies against bcl 2
1
Neuroinflammation and Oxidative Stress
2
Apoptosis Mechanism in Cancer Cells
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MTT[3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetra-zolium bromide] and cisplatin were purchased from Sigma-Aldrich (St. Louis, MO, USA). The antibodies used in this study including: antibodies against bcl-2, bax, bak, were purchased from Abcam (Cambridge, MA). Antibodies against cleaved caspase 3, mcl-1, pink1, parkin, beta-actin, HRP-linked secondary antibody were purchased from Proteintech (Chicago, IL). Antibodies against grp78, chop were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).
3
Reagents and Antibodies for Cell Experiments
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PITC and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich; Merck KGaA. N-acetyl-L-cysteine (NAC) was purchased from Beyotime Institute of Biotechnology. PITC was dissolved in DMSO to prepare stock solutions for in vitro assays and stored at −20°C. Cells in the control groups were treated with vehicle at an equal volume. To minimize toxicity, DMSO concentration was >0.1% for cell culture. Anti-poly-ADP-ribose polymerase (PARP; catalog no. 9542; 1:1,000), anti-cleaved caspase-3 (catalog no. 9662; 1:1,000), anti-cleaved caspase-9 (catalog no. 9502; 1:1,000), anti-GAPDH (catalog no. 5174; 1:1,000), anti-cytochrome c (Cyt c; catalog no. 4272; 1:1,000), anti-p53 (catalog no. 2527; 1:1,000) and anti-phosphorylated p53 (p-p53; catalog no. 2521; 1:1,000) antibodies were purchased from Cell Signaling Technology, Inc. Anti-cyclin A1 antibody (catalog no. ab118897; 1:1,000) was obtained from Abcam, Inc. Antibodies against Bcl-2 (catalog no. A2845; 1:1,000), Bax (catalog no. A0207; 1:1,000) and β-tubulin (catalog no. AC008; 1:1,000 for western blot analysis and 1:400 for immunofluorescence assay) were purchased from ABclonal Biotech Co., Ltd.
4
NAC and LY294002 Signaling Pathways
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N-acetyl-cysteine (NAC) was purchased from Sigma–Aldrich (St. Louis, MO, USA). LY294002 was supplied by Cell Signaling Technology (Danvers, MA, USA). The rabbit anti-TRIM8 and anti-GAPDH were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). These antibodies including Cleaved caspase-3, BAX, AKT, p-AKT, PI3K and p-PI3K were purchased from Cell Signaling Technology (Danvers, MA, USA), while antibodies against Bcl-2 and IL-1β were obtained from Abcam (Cambridge, UK). Primary antibodies against NLRP3, Caspase-11 and Caspase-1 and ASC and GSDMD-N were supplied by Santa Cruz Biotechnology (Santa Cruz, CA, USA).
5
Apoptosis Pathway Profiling Protocol
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Antibodies against caspase-3 (#56053) and compound ABT-737 were purchased from Santa Cruz Biotechnology, Inc.; antibodies against AKT (#4691), p-AKT (#4060), ERK (#4695), p-ERK (#4370), NF-κB (#8242), p-NF-κB (#3033), E-cadherin (#3195), vimentin (#5741), N-cadherin (#4068), caspase-8 (#4790), caspase-9 (#9504) and IAP family proteinsc-IAP1 (#7065), c-IAP2 (#3130), survivin (#2808), XIAP (#2045) and livin (#5471) were obtained from Cell Signaling Technology, Inc. Antibodies against β-actin (#A2228) and NFE2L3(#HPA055889) were obtained from Merck KGaA. Antibodies against Bcl-2 (#32124), Mcl-1 (#32087), Bad (#32245), and Bak (#32371) were purchased from Abcam. All antibodies were diluted at 1:1000. Annexin V-FITC was purchased from BD Biosciences. JC-1 was obtained from ThermoFisher Scientific, Inc. Doc was purchased from Selleck Chemicals. All other reagents were from Sigma-Aldrich; Merck KGaA. All drugs were dissolved in DMSO and stocked at -80°C.
6
Investigating Autophagy and Apoptosis Pathways
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D-galactose was purchased from Shanghai Yuan Ye Bio-Technology Co., Ltd. (Shanghai, China), MTT [3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetra-zolium bromide] was purchased from Sigma-Aldrich (St. Louis, MO, USA). The antibodies used in this study including antibodies against Bcl-2, Bax, Bak, p62, LC3 I/II were purchased from Abcam (Cambridge, MA, USA). Antibodies against Cleaved caspase 3, Mcl-1, Pink, Parkin, Beta-actin, HRP-linked secondary antibody were purchased from Proteintech (Chicago, IL, USA). Antibodies against AKT, p-AKT, 4EBP1, p-4EBP1, p70s6k, p-p70s6k were purchased from Cell Signaling Technology (Boston, MA, USA).
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