9180 electrolyte analyzer

Manufactured by Roche

Sourced in Germany, Switzerland

The 9180 Electrolyte Analyzer is a laboratory instrument designed for the measurement of electrolyte concentrations in various bodily fluids. It provides accurate and reliable analysis of key electrolytes such as sodium, potassium, and chloride.

Automatically generated - may contain errors

Lab products found in correlation

Ysi 2300, by Yellow Springs Instruments (2 mentions) Triton x 100, by Avantor (2 mentions) Cobas integra 400 plus, by Roche (1 mentions) Silverquest silver staining kit, by Thermo Fisher Scientific (1 mentions) Eclipse 80i, by Nikon (1 mentions) Lactate pro 2 analyzer, by Arkray (1 mentions) 7180 clinical analyzer, by Hitachi (1 mentions) Protein assay, by Bio-Rad (1 mentions) Edta vacutainer, by BD (1 mentions) Fiske os osmometer, by Advanced Instruments (1 mentions) Pentra dx 120, by Horiba (1 mentions) Insyte w, by BD (1 mentions) Statplus, by AnalystSoft (1 mentions)

18 protocols using 9180 electrolyte analyzer

Serum Lithium Concentration Measurement

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serum lithium concentrations were measured using a direct ion-selective electrode method (Cobas Integra 400 Plus (at St Olav University Hospital) or 9180 Electrolyte Analyzer (at Diakonhjemmet Hospital), both from Roche Diagnostics GmbH, Mannheim, Germany).19
20 Concentrations were reported in mmol/L with two significant digits after the decimal point. The lower limits of quantification were 0.16 mmol/L at St Olav University Hospital and 0.10 mmol/L at Diakonhjemmet Hospital.

Westin A.A., Brekke M., Molden E., Skogvoll E., Aadal M, & Spigset O. (2017). Changes in drug disposition of lithium during pregnancy: a retrospective observational study of patient data from two routine therapeutic drug monitoring services in Norway. BMJ Open, 7(3), e015738.

+ Open protocol

+ Expand

Comprehensive Renal Function Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols

Urine sediment (30 μL) was examined under a light microscope (Eclipse 80i, Nikon, Tokyo, Japan) to confirm the occurrence of crystals. Plasma osmolality was measured using an osmometer (Precision Systems, Natick, MA, United States) and sodium concentration was determined by flame photometry (9180 Electrolyte Analyzer; Roche, Basel, Switzerland) as previously described (Casare et al., 2016 (link); de Ponte et al., 2017 (link)). Plasma urea and creatinine as well as urine creatinine levels were evaluated using colorimetric tests (Labtest, Lagoa Santa, MG Brazil). The creatinine clearance was calculated using the following formula: [C = (Urine_Cr⋅V)/Plasma_Cr], where C is clearance, Cr is creatinine, and V represents urinary flow. The urinary albumin excretion was determined using a SilverQuest Silver Staining Kit (Thermo Fisher Scientific, Waltham, MA, United States) by the modified Oakley method (Oakley et al., 1980 (link)). Briefly, urine samples (volume corresponding to 5 μg creatinine) from the metabolic cages were separated by SDS polyacrylamide gel electrophoresis (10%). Next, silver staining was performed on the gel, and albumin bands were identified using a molecular weight marker (bovine serum albumin – BSA, 66 kDa). The bands were analyzed by optical densitometry using ImageJ software [National Institutes of Health (NIH), Bethesda, MD, United States].

de Araújo L., Costa-Pessoa J.M., de Ponte M.C, & Oliveira-Souza M. (2020). Sodium Oxalate-Induced Acute Kidney Injury Associated With Glomerular and Tubulointerstitial Damage in Rats. Frontiers in Physiology, 11, 1076.

+ Open protocol

+ Expand

Metabolic and Renal Function Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols

At the end of six weeks, animals from all the experimental groups were housed in metabolic cages for 24 h urine collection. Then, the animals were anesthetized with ketamine and xylazine, and aortic blood samples were collected. The plasma and urinary concentrations of sodium, potassium (9180 Electrolyte Analyzer, Roche Diagnostics, Indianapolis, USA), and creatinine (Labtest Diagnostics, Lagoa Santa, Brazil) and the urinary excretion of protein (Labtest Diagnostics, Lagoa Santa, Brazil) were determined. Both kidneys were harvested, the cortex and medulla were quickly separated on ice, and the fragments were destined for gene expression analysis.

Ishiy C.S., Ormanji M.S., Maquigussa E., Ribeiro R.S., da Silva Novaes A, & Boim M.A. (2020). Comparison of the Effects of Mesenchymal Stem Cells with Their Extracellular Vesicles on the Treatment of Kidney Damage Induced by Chronic Renal Artery Stenosis. Stem Cells International, 2020, 8814574.

+ Open protocol

+ Expand

Rigor Mortis Development in Fish

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood samples were immediately extracted from the caudal vein using lithium heparinised syringes (n = 30). The blood lactate was measured immediately using a Lactate Pro 2 analyzer (Arkray Factory Inc., Japan). The plasma was prepared by centrifugation (9500 g, 1 min, 6 °C, Eppendorf, 5415R, Hamburg, Germany), frozen in liquid nitrogen, and further stored at -80 °C until analysed. The plasma levels of Na + , K + and Cl -were then analysed on a 9180 Electrolyte Analyzer (Roche Diagnostics GmbH, Germany). Hct was obtained using heparinized micro capillary tubes and a Compur M1100 Hct centrifuge.
The development of rigor mortis was measured by Cuttingers Method (tail drop) (Bito, 1983) .
The rigor index (Ir) was calculated by the formula Ir = [(Lo-Lt)/Lo] × 100, where L represents the vertical drop (cm) of the tail when half of the fish fork length is placed on the edge of a table as a function of time. The tail drop at the beginning of the experiment is Lo, while Lt represents measurements throughout the experiment (t = 0-60 hours with interval of 4 hours).

Lerfall J., Hasli P.R., Skare E.F., Olsen R.E., Rotabakk B.T., Roth B., Slinde E, & Egelandsdal B. (2017). A comparative study of diploid versus triploid Atlantic salmon (Salmo salar L.). The effects of rearing temperatures (5, 10 and 15°C) on raw material characteristics and storage quality. Food chemistry, 225.

+ Open protocol

+ Expand

Blood Sample Processing and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood samples were drawn into 5-mL heparin syringes (Terumo Corporation, Leuven, Belgium). 200 μL of blood was immediately haemolysed in 200 μL of ice-cold Triton X-100 buffer solution (Triton X-100, Amresco, Salon, OH) and analysed to determine blood [lactate] and [glucose] within ~5 min of collection (YSI 2300, Yellow Springs Instruments, Yellow Springs, OH). The remaining whole blood was then centrifuged at 4000 rpm for 3 min (Hetting EBA 20, Tuttlingen, Germany) before plasma was extracted and stored on ice for ~30 min prior to being frozen at -80 °C for subsequent analysis of plasma [K⁺] and [Na⁺] (9180 Electrolyte Analyzer, F. Hoffmann-La Roche, Basel, Switzerland). Blood samples for pH and bicarbonate analyses were drawn in 2.5-mL syringes and analysed instantly by an ABL acid-base analyser (Radiometer, Brønshøj, Denmark) [22 (link)].

Krustrup P., Ermidis G, & Mohr M. (2015). Sodium bicarbonate intake improves high-intensity intermittent exercise performance in trained young men. Journal of the International Society of Sports Nutrition, 12, 25.

+ Open protocol

+ Expand

Salivary Ion Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The saliva collected was analyzed for the concentrations of potassium (K+), sodium (Na+). For the determination of salivary ions, saliva was diluted at either 1/100 or 1/1000 and K+, Na + concentrations were determined using Roche 9180 electrolyte analyzer.

Dhanarathna S., Jeddy N., Sabarinath B, & Protyusha G.B. (2021). Estimation and comparison of salivary flow rate and its composition in diabetic patients and nondiabetic patients: A pilot study. Journal of Oral and Maxillofacial Pathology : JOMFP, 25(2), 232-238.

+ Open protocol

+ Expand

Osteoporosis Development and Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols

To verify osteoporosis development and treatment efficacy, sera were collected at 3 months after OVX and at the end of the experiment. Collected sera were tested for serum OCN concentration using ELISA, for serum alkaline phosphatase (ALP) level using a Hitachi Clinical Analyzer 7180 (Hitachi Ltd., Tokyo, Japan), and for serum total calcium concentration using a 9180 Electrolyte Analyzer (Roche Diagnostics, Basel, Switzerland). The final serum concentrations were adjusted with respect to the initial concentrations.

Kim G., Park Y.S., Lee Y., Jin Y.M., Choi D.H., Ryu K.H., Park Y.J., Park K.D, & Jo I. (2018). Tonsil-derived mesenchymal stem cell-embedded in situ crosslinkable gelatin hydrogel therapy recovers postmenopausal osteoporosis through bone regeneration. PLoS ONE, 13(7), e0200111.

+ Open protocol

+ Expand

Electrolyte and Renal Function Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The 24-h urine and plasma samples were used for creatinine and sodium quantification for the evaluation of the glomerular filtration rate (GFR) and sodium excretion fraction. Plasma and urinary creatinine levels were determined using a commercial kit (Labtest, Lagoa Santa, Brazil). Urinary and plasma sodium levels were analysed using a quantitative electrode quantification technique (9180 Electrolyte Analyzer, Roche Diagnostics GmbH, Mannheim, Germany). Urinary osmolality was determined by the freezing method (The Advanced Osmometer, Model 3250; Advanced Instruments, Norwood, USA). Plasma calcium and creatine kinase were determined using a commercial kit (Labtest, Lagoa Santa, Brazil and Laborlab, São Paulo, Brazil respectively).

Reis N.G., Francescato H.D., de Almeida L.F., Silva C.G., Costa R.S, & Coimbra T.M. (2019). Protective effect of calcitriol on rhabdomyolysis-induced acute kidney injury in rats. Scientific Reports, 9, 7090.

+ Open protocol

+ Expand

Creatinine and Electrolyte Measurement

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasma and urinary creatinine levels were measured by the Jaffé alkaline picrate assay (VALTEK Diagnostica, Santiago, Chile). Urinary protein concentration was determined by Bradford’s method (Bio-Rad protein assay, Kidlington, UK) [62 (link)]. Creatinine clearance was calculated according to the standard formula C = (U × Ṽ/P), where C is the creatinine clearance, U is the creatinine urinary concentration, Ṽ is the urine flow rate per minute, and P is the creatinine plasmatic concentration [59 (link)]. In the samples of urine and plasma, the amounts of Na⁺ and K⁺ were measured using the electrolyte meter 9180 Electrolyte Analyzer (Roche). To determine the fractional excretion of Na⁺ and K⁺, the following equation was applied:

FE {Na}^{+} or K^{+} = \frac{(PC * [{Na}^{+}] U or [K^{+}] U)}{(CU * [{Na}^{+}] P or [K^{+}] P)} * 100

where, [Na⁺]U or [K⁺]U = Concentration of Na⁺ or K⁺ in urine; and [Na⁺]P or [K⁺]P = Concentration of Na⁺ or K⁺ in plasma. PC = Plasma creatinine and CU = Creatinine in urine.
To determine the ratio U prot/U Crea, the value for urinary protein was divided by the value for urinary creatinine.

Gómez G.I., Velarde V, & Sáez J.C. (2019). Role of a RhoA/ROCK-Dependent Pathway on Renal Connexin43 Regulation in the Angiotensin II-Induced Renal Damage. International Journal of Molecular Sciences, 20(18), 4408.

+ Open protocol

+ Expand

Venous Blood Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Venous blood samples were collected into lithium-heparin (LH) and ethylenediaminetetraacetic-acid (EDTA) vacutainers (Becton–Dickinson, New Jersey, USA) on seven occasions throughout laboratory sessions three and four (Fig. 1). Immediately after sampling, 200 µL of blood was extracted from the LH vacutainer into 200 µL of Triton-X-100 solution (Triton X-100, Amresco, Salon, OH) and plasma lactate and glucose concentrations were measured ([lactate] and [glucose], respectively) (YSI 1500; Yellow Springs Instrument, Yellow Springs, OH). The remaining blood was centrifuged at 4000 rpm for 8 min at 4 °C after which plasma was extracted and analyzed for potassium and sodium concentrations ([K⁺] and [Na⁺], respectively) (9180 Electrolyte Analyzer, F. Hoffmann-La Roche, Basel, Switzerland). The plasma obtained from the EDTA vacutainers was frozen at − 80 °C and, upon completion of the study, defrosted at room temperature and analysed for cortisol concentration ([cortisol]) using an ELISA kit (Abnova, Taiwan).

Clark I.E., Goulding R.P., DiMenna F.J., Bailey S.J., Jones M.I., Fulford J., McDonagh S.T., Jones A.M, & Vanhatalo A. (2018). Time-trial performance is not impaired in either competitive athletes or untrained individuals following a prolonged cognitive task. European Journal of Applied Physiology, 119(1), 149-161.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!