Ranbp3

The cell lysates were prepared as previously described,^[28] and the protein concentration was determined with a BCA kit (Thermo Fisher Scientific). Protein samples were separated by sodium dodecyl sulfate (SDS)‐PAGE and transferred to PVDF membranes (Millipore, Bedford, MA, USA). After blocking with 5% fat‐free milk for 1 h, the PVDF membrane was incubated with primary antibody for 1–2 h at room temperature and then washed with 1× tris‐buffered saline with Tween (TBST) for 30 min. Next, the membrane was incubated with the corresponding horseradish peroxidase‐conjugated secondary antibodies for 1 h. After washing with TBST, signals were detected using ECL substrate (Bio–Rad, Hercules, CA, USA). The antibodies used included actin and RanBP3 from Santa Cruz Biotechnology (Santa Cruz, CA, USA), CDK4 and p‐Rb from Cell Signaling Technology (Beverly, MA, USA), cyclin D1, CDK6, GAPDH, histone H3, lamin B1, β‐catenin, and c‐Myc from Proteintech (Rosemont, IL, USA).