Acrylamide and bis-Acrylamide were from SERVA (Heidelberg, Germany). Immobiline DryStrips, Pharmalyte buffer (broad pH range 3–10), Immobiline DryStrip cover fluid and Amersham™ CyDye DIGE Fluors (minimal dyes) for 2D-DIGE were purchased from GE Healthcare (Piscataway Township, NJ). Complete protease inhibitor cocktail was from Roche Diagnostic (Mannheim, Germany). All others chemicals were of the highest purity available and purchased from Sigma-Aldrich (St. Louis, USA).
Acrylamide
Manufactured by Serva Electrophoresis
Sourced in Germany, United States
Acrylamide is a chemical compound used in the production of polyacrylamide gels for electrophoresis applications. It serves as the primary component in the formation of these gels, which are used to separate and analyze biomolecules, such as proteins and nucleic acids, based on their size and charge.
Automatically generated - may contain errors
Lab products found in correlation
Immobiline drystrip, by GE Healthcare (3 mentions)
Bis acrylamide, by Serva Electrophoresis (3 mentions)
Complete protease inhibitor cocktail, by Roche (3 mentions)
N n methylenebisacrylamide, by Serva Electrophoresis (3 mentions)
Immobiline drystrip cover fluid, by GE Healthcare (2 mentions)
Pharmalyte buffer, by GE Healthcare (2 mentions)
Asolectin, by Merck Group (2 mentions)
Celite 535, by Carl Roth (2 mentions)
Hydroxylapatite bio gel htp, by Bio-Rad (2 mentions)
Amberlite bio beads sm 2, by Bio-Rad (2 mentions)
Triton x 114, by Serva Electrophoresis (2 mentions)
14c 2 oxoglutarate, by PerkinElmer (2 mentions)
14c malate, by PerkinElmer (2 mentions)
Nitrocellulose membrane, by Cytiva (1 mentions)
Coomassie blue g 250, by Serva Electrophoresis (1 mentions)
Triton x 100, by Serva Electrophoresis (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Cardiolipin, by Merck Group (1 mentions)
Hemin, by Merck Group (1 mentions)
Triton x 100, by Bio-Rad (1 mentions)
6 protocols using acrylamide
1
Two-Dimensional Gel Electrophoresis Reagents
2
Gel-Based Protein Identification Workflow
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Acrylamide, bis-Acrylamide and Coomassie Blue G-250 were from SERVA (Heidelberg, Germany), nitrocellulose membrane was from Whatman (Germany). Immobiline Dry-Strips, Pharmalyte buffer, and secondary anti-rabbit antibody labeled with horseradish peroxidase were purchased from GE Healthcare (Piscataway, NJ). Complete protease inhibitor cocktail was from Roche Diagnostic, Mannheim, Germany (cat. no. 1697498). All others chemicals were from Sigma-Aldrich and were of highest purity available. Primary antibody oriented against trimeric Gβ subunit protein (T-20, sc-378) was from Santa Cruz.
3
Isolation and Characterization of Mitochondria
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Hydroxylapatite (Bio-gel HTP) and Amberlite Bio-Beads SM-2 was obtained from Bio-Rad; Matrex Gel Orange was obtained from Amicon (Beverly, MA, USA); Triton X-100, Triton X-114, acrylamide, and N,N′-methylenebisacrylamide were obtained from Serva; egg-yolk phospholipids were obtained from Fluka; hemin, cardiolipin, 1,4-piperazine-diethanesulphonic acid (Pipes), Sodium dodecyl sulfate, (SDS) and asolectin were obtained from Sigma; celite 535 was obtained from Roth; Sephadex G-75 was obtained from Pharmacia; [14C] 2-oxoglutarate and [14C] malate were purchased from Perkin-Elmer Life Sciences (Waltham, MA, USA). All other chemicals used were of analytical grade.
4
Purification and Analysis of Mitochondrial Enzymes
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In regards to chemicals: [14C] 2-oxoglutarate and [14C] malate were purchased from Perkin-Elmer Life Sciences; hydroxylapatite (Bio-gel HTP) and Amberlite Bio-Beads SM-2 from Bio-Rad; Triton X-100, Triton X-114, acrylamide and N,N′-methylenebisacrylamide from Serva; egg yolk phospholipids from Fluka; Matrex Gel Orange from Amicon (Beverly, MA, USA); hemin, cardiolipin, 1,4-piperazine-diethanesulphonicacid (Pipes), sodium dodecyl sulfate (SDS) and asolectin from Sigma; celite 535 from Roth and Sephadex G-75 from Pharmacia. All other chemicals used were of analytical grade.
5
Two-Dimensional Protein Electrophoresis
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Acrylamide and bis-Acrylamide were from SERVA (Heidelberg, Germany). Immobiline DryStrips, Pharmalyte buffer (broad pH range 3–10) and Immobiline DryStrip cover fluid were purchased from GE Healthcare (Piscataway Township, NJ). Complete protease inhibitor cocktail was from Roche Diagnostic (Mannheim, Germany), [3H]-ouabain (30 mCi/mmol, NET211001MC) from Perkin Elmer and N-glycosidase F (11 365 169 001, Roche) was from Sigma-Aldrich. All others chemicals were of highest purity available and purchased from Sigma-Aldrich (St. Louis, USA).
6
Antioxidant and Protein Analysis Reagents
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Trichloroacetic acid (TCA), 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) and 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH) were purchased from Sigma-Aldrich (Munich, Germany). Bromophenol blue sodium salt (3′,3″,5′,5″-tetrabromophenol sulfophthalein sodium salt), 2-mercaptoethanol, acrylamide, N,N′-methylenebisacrylamide, sodium dodecylsulphate (SDS), and Coomassie Brilliant Blue R-250 were purchased from SERVA Electrophoresis GmbH (Munich, Germany). PageRuler Plus Protein Ladder 10 to 250 kDa was purchased from Thermo Fisher Scientific (Waltham, MA, USA), and 2-D Quant Kit from GE Healthcare Bio-Sciences (Marlborough, MA, USA). Fluorescein sodium salt was purchased from Fluka (Everett, WA, USA). Pronase E (4,000,000 PU/g) was purchased from Merck (Darmstadt, Germany). Other solvents and reagents of analytical (ACS) or HPLC grade were purchased from Sigma-Aldrich, SERVA Electrophoresis (Munich, Germany) or POCH (Gliwice, Poland).
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