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The UAS-GCaMP6s is a genetically encoded calcium indicator that can be used to monitor neuronal activity in Drosophila. It consists of a calcium-sensitive GFP variant fused to a calmodulin domain and a M13 peptide. When calcium binds to the calmodulin domain, it causes a conformational change that increases the fluorescence of the GFP, allowing for the visualization of neuronal activity.

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Lab products found in correlation

Uas tnt, by Bloomington Drosophila Stock Center (2 mentions) Uas cd4 tdgfp, by Bloomington Drosophila Stock Center (1 mentions) Uas gcamp6f, by Bloomington Drosophila Stock Center (1 mentions) Uas tdtomato, by Bloomington Drosophila Stock Center (1 mentions) Elav gal4, by Bloomington Drosophila Stock Center (1 mentions) R43d01 gal4, by Bloomington Drosophila Stock Center (1 mentions) Or67d gal4, by Bloomington Drosophila Stock Center (1 mentions) Nsyb gal4, by Bloomington Drosophila Stock Center (1 mentions) R71g01 gal4, by Bloomington Drosophila Stock Center (1 mentions) R25e04 gal4, by Bloomington Drosophila Stock Center (1 mentions) Uas nachbac, by Bloomington Drosophila Stock Center (1 mentions) Uas mcd8 gfp, by Bloomington Drosophila Stock Center (1 mentions) Uas gcamp3, by Bloomington Drosophila Stock Center (1 mentions) Ok371 gal4, by Bloomington Drosophila Stock Center (1 mentions) Uas trpa1, by Bloomington Drosophila Stock Center (1 mentions) Uas chr2, by Bloomington Drosophila Stock Center (1 mentions) Uas shits, by Bloomington Drosophila Stock Center (1 mentions) Uas kir2, by Bloomington Drosophila Stock Center (1 mentions) Uas hid, by Bloomington Drosophila Stock Center (1 mentions) Pdf gal80, by Bloomington Drosophila Stock Center (1 mentions)

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20×uas-ivs-gcamp6s (2 citations)

4 protocols using uas gcamp6s

1

Drosophila Neuronal Line Database

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Several lines (GMR57C10-Gal4, elav-Gal4, UAS-GCaMP6s, UAS-GCaMP6f, UAS-CD4:tdGFP, and UAS-tdTomato) were obtained from the Bloomington Stock Center. MAN-Gal4 (VT50660-AD; VT14014-DBD) and MDN-1-Gal4 (VT44845-DBD; VT50660-AD) were provided by B. Dickson (Janelia Research Campus). DNa01-Gal4 (SS00731: GMR22C05-AD; GMR56G08-DBD), DNb06-Gal4 (SS02631: GMR20C04-AD; BJD113E03-DBD), DNg13-Gal4 (SS02538: BJD118A10-AD; BJD123E03-DBD), and DNg16-Gal4 (SS01543: BJD104A07-AD; BJD107F12-DBD) were provided by G. Rubin (Janelia Research Campus).
Chen C.L., Hermans L., Viswanathan M.C., Fortun D., Aymanns F., Unser M., Cammarato A., Dickinson M.H, & Ramdya P. (2018). Imaging neural activity in the ventral nerve cord of behaving adult Drosophila. Nature Communications, 9, 4390.
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2

Drosophila Genetic Toolkit for Neuroscience

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Flies were maintained in bottles on cornmeal molasses food obtained from Lab Express (Fly Food R, recipe available at http://lab-express.com/DIS58.pdf) at 25°C on a 12h:12h light:dark cycle. nsyb-Gal4, fruNP21-Gal4, R71G01-Gal4, R25E04-Gal4, R43D01-Gal4, or67d-Gal4, UAS-TNT, UAS-mCD8::GFP, UAS-NaChBac, UAS-GCaMP6S, and NF1 RNAi (BDSC #53322) were obtained from Bloomington stock center and outcrossed into an iso31 background. daughterless-GeneSwitch, Nf1E1, Nf1E2, Alk8 and Alk9 mutant alleles, UAS-dNf1 and UAS-GRD (Lorén et al., 2003 (link); Walker et al., 2006 (link); Tricoire et al., 2009 (link)) were previously described. Wild-type Canton-S flies were generously provided by Dr. Edward Kravitz. K33, Nf1P1 and Nf1P2 mutant alleles (The et al., 1997 (link)) were generously provided by Dr. Amita Sehgal and outcrossed into an iso31 background. Gr32a−/− was generously provided by Dr. Hubert Amrein. Drosophila simulans stock was generously provided by Dr. Jean-Christophe Billeter. ppk23-Gal4/CyO was generously provided by Dr. Barry Dickson. ppk23-Gal4/TM6,Sb was generously provided by Dr. Kristin Scott.
Moscato E.H., Dubowy C., Walker J.A, & Kayser M.S. (2020). Social Behavioral Deficits with Loss of Neurofibromin Emerge from Peripheral Chemosensory Neuron Dysfunction. Cell reports, 32(1), 107856.
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3

Drosophila neuronal and muscular reporters

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Following lines were used: OK371-Gal4 (genotype w1118;OK371-Gal4;+, Bloomington #26160) for targeting glutamatergic neurons, MHC-tauGFP [22 (link)] as a muscle reporter line, MT11-Gal4 (genotype w;+;P{GawB}MT11)(Bloomington #37295), UAS-mCD8::mRFP (genotype y,w;UAS-mCD8::mRFP;+, Bloomington #27398 and y,w;+;UAS-mCD8::mRFP) (Bloomington #27399 (3rd Chr.)),10xUAS-mCD8::GFP(genotype w;+;10xUAS-mCD8::GFP)(Bloomington #32184) UAS-TrpA1 (genotype w;UAS-TrpA1;+) (Bloomington #26263), UAS-shiTS (w;+;UAS-shiTS [39 (link)]), UAS-ChR2(H134R) (w;UAS-ChR2(H134R);+, Bloomington #28995), UAS-eNpHR (w;+;UAS-eNpHR::YFP, Bloomington #41752 gift from Leslie Griffith), UAS-PaGFP(A206K) (w;+;UAS-PaGFP(A206K), gift from A.S. Chiang [40 ]), UAS-GCamP3 (genotype w;+;UAS-GCamP3, Bloomington #32236), UAS-GCamP6s (genotype w1118;UAS-GCamP6s;+, Bloomington #42746), OregonR (genotype +/w;+/UAS-TrpA1;+/+). For PaGFP experiments genotype was y,w/w;UAS-mCD8::mRFP; UAS-PaGFP(A206K).
Hückesfeld S., Schoofs A., Schlegel P., Miroschnikow A, & Pankratz M.J. (2015). Localization of Motor Neurons and Central Pattern Generators for Motor Patterns Underlying Feeding Behavior in Drosophila Larvae. PLoS ONE, 10(8), e0135011.
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4

Drosophila Neurophysiology Experimental Protocol

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Flies were maintained on a media of sucrose, yeast, molasses, and agar under 12:12 LD cycles at 25 °C. One- to 3-day-old female flies were separated and maintained on standard cornmeal-yeast medium under 12:12 LD cycles at 25 °C for 4 nights before experiments began. Clk[out] (56754), pers (80919), pdf-Gal4 (6899), pBDP (pBDP-Gal4Uw)(68384), pBDP split (p65-AD Uw; Gal4-DBD Uw) (79603), R23E10-Gal4 (49032), R69F08-Gal4 (39499), R58H05 p59AD (70750), R48H04 DBD (69353) pdf-Gal80 (80940), R51H05 p65AD (70720), R18H11 DBD (69017), R92H07-Gal4 (40633), R52B02-Gal4 (38814), R20D01-Gal4 (48889), BRPstar (55751),UAS-GCaMP6s (42746), UAS-TNT (28838), UAS-kir2.1 (6596) and UAS-hid (65403) were obtained from the Bloomington Drosophila Stock Center. mGluR-RNAi (1793) was obtained from Vienna Drosophila Resource Center. MB122B and 20xUas-IVS-Syn-GFP was obtained from Janelia Farm.
Andreani T., Rosensweig C., Sisobhan S., Ogunlana E., Kath W, & Allada R. (2022). Circadian programming of the ellipsoid body sleep homeostat in Drosophila. eLife, 11, e74327.
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