Total RNA was extracted using the mirVana miRNA isolation kit according to the manufacturer’s protocol (Invitrogen, Waltham, MA). The RNA quality was analyzed using a 2100 Bioanalyzer Instrument (Agilent Technologies, Santa Clara, CA) and concentration was measured using a Qubit fluorometer (Life Technologies, Carlsbad, CA). For sRNA and mRNA sequencing, the total RNA was sent for library preparation and paired-end sequencing at the National Genomics Infrastructure (NGI), Stockholm, Sweden. The sRNA library was generated using TruSeq small RNA kit (Illumina, San Diego, CA), while the mRNA library was generated using a TruSeq Stranded mRNA Poly(A) selection kit (Illumina, San Diego, CA). The sRNA and mRNA libraries were sequenced on a NovaSeq SP flow cell with a 2 × 50-bp reads and NovaSeqXp workflow in S4 mode flow cell with 2 × 151 setup, respectively, using Illumina NovaSeq6000 equipment at NGI Stockholm. The Bcl to FastQ conversion was performed using bcl2fastq_v2.19.1.403 from the CASAVA software suite (126 (link)). The quality scale used was Sanger/phred33/Illumina 1.8+.
Truseq stranded mrna poly a selection kit
Manufactured by Illumina
Sourced in United States
The TruSeq Stranded mRNA Poly(A) selection kit is a laboratory equipment product designed for the isolation and purification of messenger RNA (mRNA) from total RNA samples. The kit utilizes oligo-dT magnetic beads to selectively capture and enrich the poly(A) tailed mRNA molecules, allowing for the removal of unwanted ribosomal RNA and other non-polyadenylated RNA species.
Automatically generated - may contain errors
Lab products found in correlation
Truseq small rna kit, by Illumina (3 mentions)
2100 bioanalyzer instrument, by Agilent Technologies (3 mentions)
Qubit fluorometer, by Thermo Fisher Scientific (3 mentions)
Mirvana mirna isolation kit, by Thermo Fisher Scientific (3 mentions)
Novaseq 6000, by Illumina (3 mentions)
Rna screentape analysis kit, by Agilent Technologies (1 mentions)
4150 tapestation system, by Agilent Technologies (1 mentions)
Novaseq 6000 sequencer, by Illumina (1 mentions)
Novaseq sp, by Illumina (1 mentions)
4 protocols using truseq stranded mrna poly a selection kit
1
RNA Extraction and Sequencing Protocol
2
Sequencing of SARS-CoV-2 Infected U4.4 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Large RNA isolated from infected U4.4 cells was quantified and quality-controlled with the 4150 TapeStation System using the RNA ScreenTape Analysis kit (Agilent Technologies, Santa Clara, CA, USA). Triplicates for each infection and timepoint were submitted to SciLifeLab for library preparation using the Illumina Truseq Stranded mRNA (poly-A selection) kit (San Diego, CA, USA). The libraries were sequenced on an Illumina NovaSeq 6000 sequencer using one lane of a S4 flow cell to a depth of 30–40 million reads per sample with a read length of 2 × 151 bp.
3
RNA Extraction and Sequencing Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was extracted using the mirVana miRNA isolation kit according to the manufacturer’s protocol (Invitrogen, Waltham, MA). The RNA quality was analyzed using a 2100 Bioanalyzer Instrument (Agilent Technologies, Santa Clara, CA) and concentration was measured using a Qubit fluorometer (Life Technologies, Carlsbad, CA). For sRNA and mRNA sequencing, the total RNA was sent for library preparation and paired-end sequencing at the National Genomics Infrastructure (NGI), Stockholm, Sweden. The sRNA library was generated using TruSeq small RNA kit (Illumina, San Diego, CA), while the mRNA library was generated using a TruSeq Stranded mRNA Poly(A) selection kit (Illumina, San Diego, CA). The sRNA and mRNA libraries were sequenced on a NovaSeq SP flow cell with a 2 × 50-bp reads and NovaSeqXp workflow in S4 mode flow cell with 2 × 151 setup, respectively, using Illumina NovaSeq6000 equipment at NGI Stockholm. The Bcl to FastQ conversion was performed using bcl2fastq_v2.19.1.403 from the CASAVA software suite (126 (link)). The quality scale used was Sanger/phred33/Illumina 1.8+.
4
Total RNA Extraction and Sequencing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was extracted using the mirVana miRNA isolation kit following the manufacturer's protocol (Invitrogen, Waltham, MA). The RNA quality was analyzed using a 2100 Bioanalyzer Instrument (Agilent Technologies, Santa Clara, CA) and concentration was measured using a Qubit fluorometer (Life Technologies, Carlsbad, CA). For sRNA and mRNA sequencing, the total RNA was sent for library preparation and paired-end sequencing at the National Genomics Infrastructure (NGI) Stockholm, Sweden. The sRNA library was generated using TruSeq small RNA kit (Illumina, San Diego, CA), while the mRNA library was generated using TruSeq Stranded mRNA poly-A selection kit (Illumina, San Diego, CA). The sRNA and mRNA libraries were sequenced on one NovaSeq SP flowcell with a 2x50 bp reads and NovaSeqXp workflow in S4 mode flow cell with 2x151 setup, respectively, using Illumina NovaSeq6000 equipment at NGI Stockholm. The Bcl to FastQ conversion was performed using bcl2fastq_v2.19.1.403 from the CASAVA software suite [72] . The quality scale used was Sanger / phred33 / Illumina 1.8+. The raw data was submitted to ENA in the bioproject PRJEB43636.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!