Phospho jnk 9251

The antibodies and reagents used in this study were as follows: GFP (A6455; rabbit; Invitrogen; Chen et al., 2011 (link)); MAP2 (AB5622; rabbit; EMD Millipore; Chen et al., 2011 (link)); MAP2 (M4403; mouse; Sigma-Aldrich; Chen et al., 2011 (link)); SMI-312R (SMI-312R; mouse; Covance; Liu et al., 2013 (link)); HA (3F10; rat; Roche; Chen et al., 2017 (link)); phospho-P38 MAPK (9211; rabbit; Cell Signaling Technology); rabbit polyclonal P38 MAPK antibody (9212; rabbit; Cell Signaling Technology); phospho-ERK (4376; rabbit; Cell Signaling Technology); ERK (4695; rabbit; Cell Signaling Technology); phospho-JNK (9251; rabbit; Cell Signaling Technology); JNK (9252; rabbit; Cell Signaling Technology); phospho-TAK1 (9339; rabbit; Cell Signaling Technology); GAPDH (sc-25778; rabbit; Santa Cruz Biotechnology, Inc.; Chen et al., 2011 (link)); NeuN (MAB377; mouse; EMD Millipore; Wang et al., 2015 (link)); HRP-conjugated secondary antibodies (GE Healthcare); and Alexa Fluor 488– and Alexa Fluor 594–conjugated secondary antibodies (Invitrogen). Antibodies with validation profiles in Antibodypedia or 1DegreeBio are underlined. CL075, poly dT, poly(I:C) high molecular weight, and SB203580 were all purchased from InvivoGen. Takinib and NG 25 were purchased from Medchem Express, and (5Z)-7-oxozeaenol was purchased from Tocris.

Western blot analyses and immunofluorescence were performed as described [19 (link)]. Antibodies specific for p63 (sc-8431), YAP (sc-398182) or integrin β4 (sc-135950) were purchased from Santa Cruz Biotechnology (CA, USA); Antibody specific for FoxO3a (2497), MST1 (3682), MST2 (3952), phospho-MST1 (Thr183)/MST2(Thr180) (3681), JNK (9252), phospho-JNK (9251) or PI3 Kinase p110α (4249) were purchased from Cell Signaling Technology (MA, USA); Antibodies specific for E-cadherin (1702-1), AKT1 (1081-1), phospho-ATK(T308) (2214-1), or SOD2 (2299-1) were purchased from Epitomics (Burlingame, CA, USA). An antibody specific for HER2 (AH210) was purchased from Beyotime Biotechnology (Shanghai, China); and an antibody specific for GAPDH (340424) was purchased from Zen BioScience (Chengdu, China). Goat anti-mouse IgG-HRP (sc-2005) or goat anti-rabbit IgG-HRP (sc-2004) antibodies were purchased from Santa Cruz Biotechnology (CA, USA); Rhodamine (TRITC)–conjugated AffiniPure Donkey Anti-Rabbit IgG (711-025-152) used for immunostaining was from purchased from Jackson Immuno Research (PA, USA).