Cells were lysed in protease inhibitor-supplemented RIPA buffer by rotation at 4°C for 30 minutes, followed by centrifugation at 14,000 g for 5 minutes at 4°C. Protein concentration was determined by BCA assay (Pierce Biotechnology) according to manufacturer’s instructions. Protein lysates (10 – 20 μg) were resolved by SDS-PAGE, transferred to PVDF (polyvinylidene difluoride) membranes, and probed with the indicated primary antibodies: α-tubulin (ab4074, Abcam), β-actin (AC-15, A3854, Sigma), BLNK (2B11, sc-8003, Santa Cruz), CAD (RB18384, AP11110c-ev, Abgent), CLPP (B-12, sc-271284, Santa Cruz), CTPS1 (C-13, sc-131474, Santa Cruz), G6PD (ab76598, Abcam), GAPDH (6C5, MAB374, Millipore Sigma), Hexokinase 2 (C-14, sc-6521, Santa Cruz), HSP70 (W27, sc-24, Santa Cruz), HSP90 α/β (F-8, sc-13119, Santa Cruz), IMPDH2 (EPR8365B, ab129165, Abcam), and MTAP (EPR6893, ab126770, Abcam). Membranes were then incubated with peroxidase conjugated secondary antibodies (sc-2004 and sc-2005, Santa Cruz). We used ECL Western Blotting Substrate (Pierce Biotechnology) according to manufacturer’s instructions and the blots were visualized by autoradiography. Quantitative densitometry analysis of western blot bands was performed employing Image J version 10.2 (NIH).
Epr8365b
Manufactured by Abcam
The EPR8365B is a lab equipment product from Abcam. It is a scientific instrument used for electron paramagnetic resonance (EPR) spectroscopy. The core function of this product is to detect and analyze the properties of unpaired electrons in a sample.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using epr8365b
1
Western Blot Analysis of Cell Lysates
2
Western Blot Protein Quantification
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Cells were lysed in protease inhibitor-supplemented RIPA buffer by rotation at 4°C for 30 minutes, followed by centrifugation at 14,000 × g for 5 minutes at 4°C. Protein concentration was determined by BCA assay (Pierce Biotechnology) according to manufacturer's instructions. Protein lysates (10–20 μg) were resolved by SDS-PAGE, transferred to polyvinylidene difluoride (PVDF) membranes, and probed with the indicated primary antibodies: α-tubulin (ab4074, Abcam), β-actin (AC-15, A3854, Sigma), BLNK (2B11, sc-8003, Santa Cruz Biotechnology), CAD (RB18384, AP11110c-ev, Abgent), CLPP (B-12, sc-271284, Santa Cruz Biotechnology), CTPS1 (C-13, sc-131474, Santa Cruz Biotechnology), G6PD (ab76598, Abcam), GAPDH (6C5, MAB374, Millipore Sigma), hexokinase 2 (C-14, sc-6521, Santa Cruz Biotechnology), HSP70 (W27, sc-24, Santa Cruz Biotechnology), HSP90 α/β (F-8, sc-13119, Santa Cruz Biotechnology), IMPDH2 (EPR8365B, ab129165, Abcam), and MTAP (EPR6893, ab126770, Abcam). Membranes were then incubated with peroxidase conjugated secondary antibodies (sc-2004 and sc-2005, Santa Cruz Biotechnology). We used ECL Western Blotting Substrate (Pierce Biotechnology) according to manufacturer's instructions and the blots were visualized by autoradiography. Quantitative densitometry analysis of Western blot bands was performed employing Image J version 10.2 (NIH).
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