Anti neun ab177487

After dewaxing and dehydration, sections were incubated with 3% hydrogen peroxide at RT for 10 min, followed by washing with PBS for three times. Then, the sections were incubated with anti-NeuN (ab177487, 1/1000, Abcam, Cambridge, UK) at 37°C for 1 h, washed with PBS again for three times, added horseradish peroxidase, and kept at 37°C for 40 min. Then, 3, 3′-diaminobenzidine (DAB) and hematoxylin staining was done and sealed.

Anti-Map2 (ab32454), anti-vGlut2 (ab79157), anti-Tubb3 (Tuj1) (ab18207), anti-NeuN (ab177487), anti-Synapsin I (ab8) and anti-H3K27Me2&Me3 (ab6147) antibodies are from Abcam. Antibody against β-actin (clone AC-74) (A5316), DMEM high glucose (D5671), Fetal Bovine Serum (F0804), Penicillin-Streptomycin (P0781), MEM non-essential amino acid solution (M7145), CHIR99021 (SML1046), Forskolin (F3917) and FGF-Basic (F0291) are from Sigma-Aldrich. Anti-GAPDH HRP (HRP 60004) is from Proteintech. Anti-total Actin HRP (sc-1615) is from Santa Cruz. Anti-Brg1 antibody is from Dr. Anki Ӧstlund-Farrants Lab (Department of Molecular Biosciences, University of Stockholm, Sweden). Hoechst 43222 (H1399), Maxima SYBR Green qPCR Master Mix (K0252), RevertAid First Strand cDNA Synthesis Kit (K1622), anti-mouse IgG Dylight 550 (84540), anti-rabbit IgG Dylight 550 (84541), smooth muscle α-actin (α-SMA) antibody (MA5-11547), Glutamax Supplement (35050061), Neurobasal medium (21103049), N2 supplement (17502048) and B27 supplement (17504044) are from Thermo Fisher Scientific. I-BET 151 (4650) and ISX 9 (4439) are from Tocris Biosciences. Trace Elements B (25-022-CI) and Matrigel matrix growth factor reduced (354230) are from Corning Inc.

Anti-PGC-1α (ab191838), Anti-Bcl-2 (ab196495), Anti-NeuN (ab177487), and Anti-Bax (ab32503) were purchased from Abcam Ltd. (Abcam, Cambridge, UK). Cytochrome C (#4272) was purchased from Cell Signaling Technology (Boston, MA, USA). The TUNEL Assay Kit was purchased with the In Situ Cell Death Detection Kit, POD (11684817910, Roche Applied Science). The ATP Content Assay Kit was purchased from Solarbio Life Science (BC0305, Beijing Solarbio Science & Technology Co., Ltd., Beijing, China). H&E staining solution (BL700B) was purchased from Biosharp Life Sciences. The immunohistochemistry kits (SV0002) were purchased from BOSTER (BOSTER Biological Technology Co., Ltd., Wuhan, China). The DAB color development solution (ZLI-9017) was purchased from Beijing ZhongShan JinQiao Company. The proteinase K solution (P1121) was purchased from Beijing Solarbio Technology Co., Ltd.