4 mm ghx nanoprobe

Manufactured by Agilent Technologies

Sourced in United States

The 4-mm gHX NanoProbe is a compact, high-performance nuclear magnetic resonance (NMR) probe designed for Agilent NMR spectrometers. It provides high-resolution analysis of small-volume samples. The probe features a 4-mm sample diameter and utilizes Agilent's gHX technology for enhanced sensitivity and efficient experimentation.

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Lab products found in correlation

Tsp d4, by Merck Group (2 mentions) Agilent 600 mhz nmr spectrometer, by Agilent Technologies (2 mentions) 600 mhz library database, by Chenomx (2 mentions) Nmr suite 7.1 professional, by Chenomx (2 mentions) Agilent 600 mhz spectrometer, by Agilent Technologies (1 mentions) Simca p 12, by Sartorius (1 mentions) Nmr suite 7, by Chenomx (1 mentions)

7 protocols using 4 mm ghx nanoprobe

Metabolomic Profiling of Beef Samples

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Beef meat samples (25 mg) were used for metabolomics profiling by NMR. Briefly,
samples were transferred to 4-mm NMR nanotubes with 25 µL deuterium oxide
containing 2 mM 3-(trimethylsilyl) propionic-2,2,3,3-d₄ acid sodium
salt (TSP-d₄; Sigma Aldrich, St. Louis, MO, USA) as an internal
standard. The NMR spectra for meat samples were acquired by a 600 MHz Agilent
NMR spectrometer (Agilent Technologies, Palo Alto, CA, USA) with a 4-mm gHX
NanoProbe for high-resolution magic angle spinning at Pusan National University
in Korea. Data were collected at a spinning rate of 2,000 Hz. A
Carr-Purcell-Meiboom-Gill pulse sequence was used to reduce the background
signals of water and macromolecules in the tissues. The ¹H-NMR
spectra were measured using 13 µs of a 90°C pulse, 0.065 s of
bigtau, 2 s of relaxation delay, 1.704 s of acquisition time, and 10 min 20 s of
total acquisition time. The TSP-d₄ peak at 0.0 ppm was used for
reference to calibrate the chemical shifts. Assignment of spectra and
quantification of metabolites were accomplished by Chenomx NMR suite 7.1
software (Chenomx, Edmonton, AB, Canada).

Jeong J.Y., Baek Y.C., Ji S.Y., Oh Y.K., Cho S., Seo H.W., Kim M, & Lee H.J. (2020). Nuclear magnetic resonance-based metabolomics analysis and characteristics of beef in different fattening periods. Journal of Animal Science and Technology, 62(3), 321-333.

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Metabolic Profiling of Heart Tissue by HR-MAS NMR

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The heart sample (72 hours after MI) was weighted 20 mg and transferred to a 4-mm HR-MAS ¹H NMR sample tube (Agilent Technologies, Santa Clara, CA, USA). 25 µL of D₂O containing 2 mM 3-(trimethylsilyl) propionic-2,2,3,3-d₄ acid sodium salt (TSP-d₄) was added to the tube. Samples were measured using HR-MAS NMR spectroscopy. All the NMR spectra were acquired at a 600.167 MHz Agilent spectrometer equipped with a 4-mm gHX NanoProbe (Agilent Technologies). CPMG (Carr-Purcell-Meiboom-Gill) pulse sequence was used because of the suppression of water and high molecular mass compounds. The acquisition time was 3.0 seconds, relaxation delay was 1 second and 128 transients were collected. The total acquisition time for each sample was 13 minutes. All spectra were processed and quantitatively analyzed using Chenomx NMR Suite 7.1 professional with the Chenomx 600 MHz library database (Chenomx Inc., Edmonton, Canada).

Lee S.Y., Lee T.W., Park G.T., Kim J.H., Lee H.C., Han J.H., Yoon A., Yoon D., Kim S., Jung S.M., Choi J.H., Chon M.K., Lee S.H., Hwang K.W., Kim J., Park Y.H., Kim J.H., Chun K.J, & Hur J. (2020). Sodium/glucose Co-Transporter 2 Inhibitor, Empagliflozin, Alleviated Transient Expression of SGLT2 after Myocardial Infarction. Korean Circulation Journal, 51(3), 251-262.

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High-Resolution NMR Metabolic Profiling

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The high-resolution magic-angle spinning nuclear magnetic resonance (HR-MAS NMR) spectra were recorded using an Agilent 600 MHz spectrometer that was equipped with a 4 mm gHX NanoProbe (Agilent Technologies, Santa Clara, CA, USA). All spectra were acquired at 600.167 MHz. The acquisition time was 1.703 s, relaxation delay was 1 s, and a total of 128 scans was obtained. The Carr-Purcell-Meiboom-Gill (CPMG) pulse sequence was used for the suppression of water and compounds with high molecular mass. For data processing, Chenomx NMR Suit 7.1 professional with the Chenomx 600 MHz library database were used (Chenomx Inc., Edmonton, AB, Canada). The bin size for spectra was 0.001 ppm. The binning data were normalized to the total area. PCA, partial least-squares discriminant analysis (PLS-DA), and orthogonal partial least-squares discriminant analysis (OPLS-DA) were performed using SIMCA-P+ 12.0 (Umetrics, Malmö, Sweden). For visualization of VIP scores of metabolites and Metabolic Set Enrichment Analysis, web-based software MetaboAnalyst 3.0 (http://www.metaboanalyst.ca) was used.

Yoon C.S., Kim H.K., Mishchenko N.P., Vasileva E.A., Fedoreyev S.A., Stonik V.A, & Han J. (2018). Spinochrome D Attenuates Doxorubicin-Induced Cardiomyocyte Death via Improving Glutathione Metabolism and Attenuating Oxidative Stress. Marine Drugs, 17(1), 2.

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Quantification of Prostate Biopsy Metabolites

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As previously described [19 (link)], ultrasound-guided prostate biopsies were acquired from 30 patients [15 from benign regions of the peripheral zone, 11 from regions of low-grade (Gleason score ≤ 3 + 4) cancer, and 4 from regions of high-grade (Gleason score ≥ 4 + 3) cancer] after approval by the UCSF Institutional Review Board (IRB) and informed consent using an 18- gauge needle (15-mm X 1-mm cores). Biopsies were placed in individual cryovials and snap-frozen on dry ice (≤15 s) immediately after the procedure. The tissues were processed for HR–MAS NMR, as described by Tessem et al. [40 (link)]. Briefly, each biopsy was weighed prior to loading into an HR–MAS rotor with a known mass of 3.0 μL of deuterium oxide containing 0.75% weight/volume sodium-3- trimethylsilylpropionate-2,2,3,3-d4 (D₂O + TSP). Quantitative ¹H HR–MAS NMR was performed at 11.7 T Varian INOVA NMR with a 4-mm gHX nanoprobe (Agilent Technologies, Santa Clara, CA, USA ) at 1 °C, and 2250 Hz spin rate. Fully relaxed pulse-acquire spectra were acquired with a 2-s presaturation delay, 2-s acquisition time, 40,000 points, 20,000-Hz spectral width, and 128 transients. The data were processed and quantified using the previously published HR–QUEST technique using ERETIC, an electronic reference to access ex vivo concentrations [60 (link)], yielding absolute concentrations of lactate [40 (link)].

Sriram R., Van Criekinge M., DeLos Santos J., Ahamed F., Qin H., Nolley R., DeLos Santos R., Tabatabai Z.L., Bok R.A., Keshari K.R., Vigneron D.B., Peehl D.M, & Kurhanewicz J. (2020). Elevated Tumor Lactate and Efflux in High-grade Prostate Cancer demonstrated by Hyperpolarized 13C Magnetic Resonance Spectroscopy of Prostate Tissue Slice Cultures. Cancers, 12(3), 537.

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Metabolic Profiling of Meat Samples

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Meat samples (25 mg) were used for ¹H-NMR metabolic profiling.
Briefly, samples were transferred to 4-mm NMR nanotubes with 25 μL
deuterium oxide containing 2 mM 3-(trimethylsilyl)
propionic-2,2,3,3-d₄ acid sodium salt (TSP-d₄; Sigma
Aldrich, St. Louis, MO, USA) as an internal standard. The NMR spectra for meat
samples were acquired by a 600 MHz Agilent NMR spectrometer (Agilent
Technologies, Palo Alto, CA, USA) with a 4-mm gHX NanoProbe for high-resolution
magic angle spinning at Pusan National University in Korea. Data were collected
at a spinning rate of 2,000 Hz. A Carr-Purcell-Meiboom-Gill pulse sequence was
used to reduce the background signals of water and macromolecules in the
tissues. The ¹H-NMR spectra were measured using 13 μs of a
90° pulse, 0.065 s of bigtau, 2 s of relaxation delay, 1.704 s of
acquisition time, and 10 min 20 s of total acquisition time. The
TSP-d₄ peak at 0.0 ppm was used for reference to calibrate the
chemical shifts. Assignment of spectra and quantification of metabolites were
accomplished by Chenomx NMR suite 7.1 software (Chenomx, Edmonton, AB,
Canada).

Jeong J.Y., Kim M., Ji S.Y., Baek Y.C., Lee S., Oh Y.K., Reddy K.E., Seo H.W., Cho S, & Lee H.J. (2020). Metabolomics Analysis of the Beef Samples with Different Meat Qualities and Tastes. Food Science of Animal Resources, 40(6), 924-937.

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High-Resolution 1H NMR Metabolic Profiling of Biopsied Tissues

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All spectra were acquired at 600.167 MHz using an Agilent spectrometer operating at ¹H frequency and equipped with a 4-mm gHX NanoProbe. High-resolution ¹H NMR metabolic profiling of the biopsied tissue samples was achieved using magic angle spinning (MAS) at 54.7° with respect to the direction of the magnetic field. All data were collected at a spinning rate of 2,000 Hz, and the spectra were checked between the water peak and side band, which coincides with the spin rate. Spectra were acquired using the presat-CPMG (Carr-Purcell-Meiboom-Gill) pulse sequence to suppress water and high molecular mass compounds. The acquisition time was 1.704 sec and relaxation delay time was 1 sec. In total, 128 scans were collected at a spectral width of 9615.4 Hz at 299.1 K. A total measurement time of 8 min 13 sec was required per sample. All data were Fourier-transformed and calibrated to TSP-d4 as 0.00 ppm using Chenomx NMR Suite 7.1 professional software (Chenomx Inc., Edmonton, Canada).

Mun J.H., Lee H., Yoon D., Kim B.S., Kim M.B, & Kim S. (2016). Discrimination of Basal Cell Carcinoma from Normal Skin Tissue Using High-Resolution Magic Angle Spinning 1H NMR Spectroscopy. PLoS ONE, 11(3), e0150328.

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Quantitative Metabolic Profiling of Ginseng

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For measuring high-resolution magic angle spinning (HR-MAS) nuclear magnetic resonance (NMR) spectroscopy, each ginseng sample (3 mg) was transferred to a 4-mm HR-MAS NMR sample tube (Agilent Technologies, Santa Clara, CA, USA). Heavy water (D 2 O; 37 μL) containing 2 mM of 3-(trimethylsilyl) propionic-2,2,3,3d 4 acid sodium salt (TSP-d 4 ) was added to each sample tube.
All NMR spectra were measured using a 600.167 MHz Agilent spectrometer equipped with a 4-mm gHX NanoProbe (Agilent Technologies). The spinning rate was 2050 Hz for HR-MAS. We collected 128 transients using the Carr-Purcell-Meiboom-Gill (CPMG) pulse sequence with PRESAT for the suppression of water and high-molecular-mass compounds [21] . The one-dimensional (1D) spectra were obtained using a 1.704 s acquisition time, a 1 s relaxation delay. The TSP-d 4 peak at 0.00 ppm was used as a reference to calibrate the chemical shift [22] . And correlation spectroscopy (COSY), which is one of the homonuclear two-dimensional (2D) experiment, was recorded and processed with 128 of scans and 256 of t1 increments.

Lee W., Yoon D., Ma S., Lee D., Lee J., Jo I., Kim T., & Kim S. (2020). Machine learning for a rapid discrimination of ginseng cultivation age using 1H-NMR spectra. Applied Biological Chemistry, 63(1).

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