Peptides were separated on an Acclaim PepMap 100 C18 trap and an Acclaim PepMap RSLC C18 column (ThermoFisher Scientific), using a nanoLC Ultra 2D plus loading pump and nanoLC as-2 autosampler (Eksigent). Peptides were eluted with a gradient of increasing acetonitrile, containing 0.1% formic acid (5–40% acetonitrile in 15 min, 40–95% in a further 5 min, followed by 95% acetonitrile to clean the column, before re-equilibration to 5% acetonitrile). The eluate was sprayed into a TripleTOF 5600 electrospray tandem mass spectrometer (ABSciex) and analysed in Information Dependent Acquisition (IDA) mode, performing 200 msec of MS followed by 100 msec MSMS analyses on the 15 most intense peaks seen by MS.
Nanolc as 2 autosampler
Manufactured by AB Sciex
The NanoLC as-2 autosampler is a laboratory instrument designed for automated sample handling and injection in liquid chromatography (LC) systems. It is a core component of the NanoLC platform, responsible for the precise and consistent introduction of liquid samples into the analytical column. The NanoLC as-2 autosampler performs tasks such as sample aspiration, sample transfer, and sample injection, enabling efficient and reproducible sample analysis.
Automatically generated - may contain errors
Lab products found in correlation
Acclaim pepmap rslc c18 column, by Thermo Fisher Scientific (1 mentions)
Tripletof 5600 electrospray tandem mass spectrometer, by AB Sciex (1 mentions)
Nanolc ultra 2d, by AB Sciex (1 mentions)
Acclaim pepmap 100 c18 trap, by Thermo Fisher Scientific (1 mentions)
Nanolc ultra 2dplus system, by AB Sciex (1 mentions)
2 protocols using nanolc as 2 autosampler
1
Peptide Separation and Mass Spectrometry
2
Peptide Separation by Nano-LC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
One-dimensional (1D) chromatographic separations of peptides were performed by a nanoLC ultra 2Dplus system (Eksigent, AB SCIEX Germany GmbH, Darmstadt, Germany) coupled to a 15 cm fused-silica emitter, 75 µm inner diameter, packed with a Magic C18 AQ 5 µm resin (Michrom BioResources, Auburn, CA, USA). Peptides were loaded on the column from a cooled (4 °C) nanoLC-AS2 autosampler (Eksigent) and separated in a 60-min linear gradient of ACN (5–40%) and water containing 0.2% formic acid at a flow rate of 300 nL/min.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!