9 amino 1 2 3 4 tetrahydroacridine hydrochloride hydrate

The following reagents were obtained from Sigma (St. Louis, MO, USA); acetic acid, bicinchoninic acid solution, bovine serum albumin, copper(II) sulfate pentahydrate, Cresyl violet acetate, 3,3′-diamino-benzidine (DAB), radioimmunoprecipitation assay (RIPA), scopolamine hydrobromide, skim milk, and 9-amino-1,2,3,4-tetrahydroacridine hydrochloride hydrate (THA). The CREB, phospho-CREB, NGF, BDNF, beta-actin, and secondary horseradish peroxidase (HRP)-conjugated antibodies used for western blotting were obtained from Abcam (Cambridge, MA) and Santa Cruz Biotechnology (Santa Cruz, CA); the doublecortin (DCX), biotinylated secondary antibodies, and avidin-biotin peroxidase complex used for immunohistochemical staining were obtained from Abcam (Cambridge, MA), Santa Cruz Biotechnology (Santa Cruz, CA) and Vector Laboratories (Burlingame, CA).

(−)-Scopolamine hydrobromide (scopolamine) and 9-Amino-1,2,3,4-tetrahydroacridine hydrochloride hydrate (tacrine) were purchased from Sigma. Scopolamine and tacrine were dissolved in 0.9% saline solution for animal administration.

Acetylcholinesterase from Electrophorus electricus (Type VI-S; EC 3.1.1.7) (eelAChE), acetylcholine iodide (≥97%, ACh), choline chloride (≥99%, Ch), galanthamine hydrobromide from Lycoris sp (≥94% HPLC), 9-amino-1,2,3,4-tetrahydroacridine hydrochloride hydrate (tacrine hydrochloride, ≥99%), donepezil hydrochloride (≥98% HPLC), edrophonium chloride, caffeine, ammonium acetate, Tris hydrochloride, glutaraldehyde (25% aqueous solution), and sodium phosphate monobasic were purchased from Sigma-Aldrich (St. Louis, United States). 3-(Aminopropyl)trietoxysilane (≥99%, APTES) was purchased from Acros Organics (Geel, BE). HPLC grade methanol (MeOH), acetonitrile (ACN) and ethanol (EtOH) were acquired from Merck-Millipore (Burlington, United States). Ultrapure water was obtained with a Merck-Millipore Milli-Q^® system (Burlington, United States). Fused silica capillary (0.375 mm o. d. x 0.1 mm i. d.) was acquired from Polymicro Technologies (Phoenix, United States). All the solutions were filtered through a Merck-Millipore nylon membrane filter (0.22 μm) prior to use (Burlington, United States).