Methyl isonipecotate (≥70%, Sigma, USA), thiophene-2-thiol (≥99%, Sigma, USA), ethyl acetate (≥99.8%, Energy Chemical, China), croconic acid (>95%, Aladdin, China), n-butanol (≥99%, Aladdin, China), toluene (98%, Yishi Chemical, China), N-hydroxysuccinimide (NHS, 98%, Aladdin, China), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC, Aladdin, China), imiquimod (R837, 99%, Meryer, China), aB2MG (ABclonal, China), 4,6-biphenyl-2-phenylindole (DAPI, Beyotime, China). For immunostaining experiments, the following antibodies were used: rabbit anti-forkhead box O4 (FOXO4) (A17978, ABclonal, China), rabbit anti-p16ink4a (A0262, ABclonal, China), rabbit anti-p21CIP1 Rabbit pAb (A1483, ABclonal, China), rabbit anti-lamin B1 (A16909, ABclonal, China), rabbit anti-p53 (A0263, ABclonal, China), rabbit anti-decoy receptor 2 (DcR2) (A6136, ABclonal, China), mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (AC002, ABclonal, China), goat anti-rabbit IgG H&L Alexa Fluor® 594 (ab150080, Abcam, UK), HRP goat anti-rabbit IgG (H+L) (AS029, ABclonal, China) and HRP goat anti-mouse IgG (H+L) (ab205719, Abcam, UK).
A0263
Manufactured by ABclonal
Sourced in China, United States
A0263 is a lab equipment product designed to facilitate specific laboratory procedures. It serves a core function within the research workflow, but a detailed description while maintaining an unbiased and factual approach is not available.
Automatically generated - may contain errors
Lab products found in correlation
Ethyl acetate, by Energy Chemical (1 mentions)
Ab150080, by Abcam (1 mentions)
Ab205719, by Abcam (1 mentions)
As029, by ABclonal (1 mentions)
A1483, by ABclonal (1 mentions)
A0262, by ABclonal (1 mentions)
Ac002, by ABclonal (1 mentions)
Ab8226, by Santa Cruz Biotechnology (1 mentions)
Anti p53, by ABclonal (1 mentions)
Anti bcl2 12789 1 ap, by Proteintech (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Anti β catenin, by Abcam (1 mentions)
Ab32572, by Abcam (1 mentions)
Ab32072, by Abcam (1 mentions)
2 protocols using a0263
1
Senescence-Associated Protein Profiling
2
Western Blot Analysis of Key Cellular Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cell protein lysates were generated using RIPA Lysis Buffer (Beyotime, China). Cellular proteins were extracted 72 h after transfection. Western blotting was performed as reported previously 45 (link). Five μg proteins were boiled in 5 × SDS buffer for 5 min, separated by SDS-PAGE, and transferred to PVDF membranes (Millipore, USA). Then, the membranes were blocked with skim milk and probed with Anti-p53 (A0263, abclonal, USA), Anti-NFAT5 (Bs-9473R, bioss,China), Anti-β-catenin (Ab32572, Abcam, USA), Anti-c-myc (Ab32072, Abcam, USA), Anti-Bcl-2 (12789-1-ap, Proteintech, China). β-actin (ab8226, Santa Cruz, USA) was used as a loading control. The results were visualized with horseradish peroxidase-conjugated secondary antibodies (Santa Cruz) and enhanced chemiluminescence. All blots were performed in triplicate and protein expression levels were quantified relatively to the expression of β-actin using the Image J 1.42q software (Wayne Rasband).
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