4 nitrophenol pnp

Manufactured by Merck Group

Sourced in United Kingdom, United States

4-nitrophenol (PNP) is a chemical compound used in various laboratory applications. It is a white crystalline solid with a characteristic phenolic odor. PNP serves as a versatile reagent and intermediate in organic synthesis, analytical chemistry, and biochemical research.

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4-nitrophenol (111 citations)

4 protocols using 4 nitrophenol pnp

Inhibition of α-Glucosidase Activity by OH-PCBs

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Twenty-six OH-PCBs were purchased from J&K Chemical (Beijing, China). α-Glucosidase from S. cerevisiae (EC 3.2.1.20) and 4-Nitrophenol(PNP) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The substrate 4-nitrophenyl-α-D-glucopyranoside (PNPG) and Na₂CO₃ were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Phosphate buffer (Na₂HPO₄-NaH₂PO₄, PH6.8) was purchased from Leagene Biotechnology Co., Ltd. (Beijing, China). Ultra-performance liquid chromatography (UPLC)-grade acetonitrile was purchased from Tianjin Saifurui Technology Ltd. (Tianjin, China). Ultra-pure water was prepared by Millipore Elix 5 UV and Milli-Q Gradient Ultra-Pure Water System. All other reagents were of ultra-performance liquid chromatography (UPLC) grade or of the highest grade commercially available.

Qin G., Jia R., Xue J., Chen L., Li Y., Luo W., Wu X., An T, & Fang Z. (2024). New Perspectives on the Risks of Hydroxylated Polychlorinated Biphenyl (OH-PCB) Exposure: Intestinal Flora α-Glucosidase Inhibition. Toxics, 12(4), 237.

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Synthesis of Metal-Organic Nanocomposites

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Pyrrole (Aldrich) was kept below 5 °C in the absence of light. Anhydrous FeCl₃ 98% (Aldrich), 3-triethoxysilylpropylamine (APTES, 99%, Aldrich), FeCl₂·4H₂O (Aldrich), titanium n-butoxide (Sigma Aldrich, TBOT), NaOH pellets (LobaChemie, India) were used as received. NH₄OH solution (25 wt%), silver nitrate (BDH, UK), sodium boron hydride (NaBH₄) (Johnson Matthey, UK), 4-nitrophenol (PNP) (Sigma Aldrich), and methylene blue (MB) were used without further purification.

Amer W.A., Al-saida B, & Ayad M.M. (2019). Rational design of a polypyrrole-based competent bifunctional magnetic nanocatalyst. RSC Advances, 9(32), 18245-18255.

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Synthesis and Catalytic Applications

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Catalytic activity on reduction reaction was evaluated from the transformation of 4-nitrophenol (p-NP, 99%) supplied by Sigma-Aldrich. Selective oxidation catalysis was evaluated from the oxidation of dimethyl(phenyl)silane (C₈H₁₂Si, 98%) supplied by Sigma-Aldrich. Magnetic cobalt ferrite was synthesized using ACS reagent quality iron(ii) chloride anhydrous and cobalt chloride hexa-hydrated purchased from Sigma-Aldrich and Merck, respectively. Gold nanoparticles were synthesized using tetrachloroauric(iii) acid trihydrate (HAuCl₄·3H₂O, ≥99%) supplied by Sigma-Aldrich. Other chemicals used in nano-synthesis processes such as trisodium citrate di-hydrate, tannic acid, potassium carbonate, methylamine (40% w/w), (3-aminopropyl)triethoxysilane (APTES), and sodium dodecyl sulfate (SDS) were of analytical grade supplied by Sigma-Aldrich and Merck. Reductant agent sodium borohydride was supplied by Merck. All solutions were prepared with ultrapure water obtained from a Millipore Milli-Q system with resistivity > 18.2 MΩ cm at 25 °C.

Saire-Saire S., Barbosa E.C., Garcia D., Andrade L.H., Garcia-Segura S., Camargo P.H, & Alarcon H. (2019). Green synthesis of Au decorated CoFe2O4 nanoparticles for catalytic reduction of 4-nitrophenol and dimethylphenylsilane oxidation. RSC Advances, 9(38), 22116-22123.

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Standardized Extraction of Citrus Flavonoids

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Pericarpium Citri Reticulatae was purchased from a local herbal market. It was authenticated by Professor Xin-feng Gao. A voucher specimen was deposited in Herbaruim of Chengdu Institute of Biology, No. CIBI0064257.
Nobiletin and Tangeretin were purchased from Chengdu Must Bio-technology Co., LTD (China) and indentified in our laboratory for qualitative and quantitative analysis. β-naphthoflavone, polydiallyldimethylammonium chloride (PDDA), β-nicotinamide adenine dinucleotide phosphate hydrate (NADP), glucose-6-phosphate, yeast glucose-6-phosphate dehydrogenase, 4-nitrophenol (PNP) and 4-nitrocatechol (PNC) were purchased from Sigma (MO, USA). HPLC grade acetonitrile was purchased from Fisher Scientific (Fisher, Fair Lawn, USA). Deionized water was purified by a Milli-Q water system (Millipore Corp., Bedford, MA, USA). Tetraethyl orthosilicate (TEOS) was purchased from TCI (Tokyo, Japan). Other chemicals and solvents were of analytical reagent grade and were obtained from Chengdu Chemical Factory (Chengdu, China).

Lei J., Xue Y., Liu Y.M, & Liao X. (2017). Characterization of major metabolites of polymethoxylated flavonoids in Pericarpium Citri Reticulatae using liver microsomes immobilized on magnetic nanoparticles coupled with UPLC/MS–MS. Chemistry Central Journal, 11, 13.

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