For the suppressive assay, cryoconserved PBMCs were thawed and stained with Percp-Cy5.5–conjugated anti-human HLADR, FITC-conjugated anti-human CD33 (BD) and PE-conjugated anti-human IL4Rα (R&D Systems). For the cGMP and cAMP analysis, cryoconserved PBMCs from patients treated with “high” or “intermediate” dose of tadalafil, cells were labeled with: BV421-conjugated anti-human CD3 (e-Bioscience), FITC-conjugated anti-human CD33 (BD), PE-conjugated anti-human IL4Rα (R&D Systems), and brilliant violet 711–conjugated anti-human HLADR. Cells were sorted at the Diabetes Research Institute (DRI) flow cytometry core at the University of Miami, Miami, FL, using a BD-FACS-ARIA.
Pe conjugated anti human il4rα
Manufactured by R&D Systems
PE-conjugated anti-human IL4Rα is a fluorescently labeled antibody that specifically binds to the human interleukin-4 receptor alpha subunit (IL4Rα). It can be used to detect and quantify the expression of IL4Rα on the surface of cells by flow cytometry.
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Lab products found in correlation
1 protocol using pe conjugated anti human il4rα
1
Suppressive assay and cGMP/cAMP analysis
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