Pe cy7 conjugated mouse igg2b

Manufactured by BioLegend

PE/Cy7-conjugated mouse IgG2b is a fluorescent-conjugated antibody that can be used for flow cytometry and other immunoassay applications. It is a mouse IgG2b subclass antibody labeled with the tandem dye PE/Cy7.

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Lab products found in correlation

Facscanto ll, by BD (2 mentions) Alexa fluor 488 annexin 5 dead cell apoptosis kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Mouse IgG2b-PE IgG2b-PE Mouse IgG2b IgG2b

2 protocols using pe cy7 conjugated mouse igg2b

CD31+ Cell Identification and Isolation

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Cells (1 × 10⁶) resuspended in 100 μL PBS containing 3% FBS were stained by APC-conjugated anti–CD31 antibody (R&D Systems, Cat.# FAB3567A-025) for 30 min at room temperature, washed by PBS containing 3% FBS, and then analyzed by FACSCanto ll (BD Biosciences). PE/Cy7-conjugated mouse IgG2b (BioLegend, Cat.# 400325) was used as isotype control antibody. Flow sorting for CD31-positive cells was then confirmed with CD31 gene expression by RT-PCR.

Campisi M., Sundararaman S.K., Shelton S.E., Knelson E.H., Mahadevan N.R., Yoshida R., Tani T., Ivanova E., Cañadas I., Osaki T., Lee S.W., Thai T., Han S., Piel B.P., Gilhooley S., Paweletz C.P., Chiono V., Kamm R.D., Kitajima S, & Barbie D.A. (2020). Tumor-Derived cGAMP Regulates Activation of the Vasculature. Frontiers in Immunology, 11, 2090.

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PD-L1 Expression and Apoptosis Analysis

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1 × 10⁶ cells resuspended in 100 μl PBS containing 3% FBS were stained by PE/Cy7-conjugated anti-PD-L1 antibody (Biolegend, Cat.# 329718) for 30 min at room temperature, washed by PBS containing 3% FBS, and then analyzed by FACSCanto ll (BD Biosciences). PE/Cy7-conjugated mouse IgG2b (Biolegend, Cat.# 400325) was used as isotype control antibody. For apoptosis analysis, cells were stained by Annexin V using Alexa Fluor 488 Annexin V dead cell apoptosis kit (Thermo Fisher Scientific, Cat.# V13245) according to the manufacturer’s instructions, and then analyzed by FACSCanto ll (BD Biosciences).

Kitajima S., Ivanova E., Guo S., Yoshida R., Campisi M., Sundararaman S.K., Tange S., Mitsuishi Y., Thai T.C., Masuda S., Piel B.P., Sholl L.M., Kirschmeier P.T., Paweletz C.P., Watanabe H., Yajima M, & Barbie D.A. (2018). Suppression of STING associated with LKB1 loss in KRAS-driven lung cancer. Cancer discovery, 9(1), 34-45.

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