Cells or tissues were fixed in 4% paraformaldehyde (in 1× PBS), followed by permeabilization in 0.25% Triton X-100 for 15–30 min, then they were washed in PBS and incubated with primary antibody for at least 1 h at room temperature. Following washing in PBS, cells/tissues were incubated with Alexa Fluor–conjugated secondary antibodies. Alexa Fluor–conjugated phalloidin was purchased from Invitrogen (A12381). Rabbit anti–IL-1β (ab9722) and anti–caspase-1 (ab62698) antibodies were purchased from Abcam. Rabbit anti–gasdermin D (96458), cleaved gasdermin D (36425), and cleaved IL-1β (83186) were purchased from Cell Signaling Technology. Cells or tissues were mounted with VECTASHIELD mounting medium (H-1200; Vector Laboratories) containing DAPI, and images were captured using an Olympus IX83 inverted fluorescence microscope or a Zeiss LSM confocal microscope. Images were adjusted for brightness/contrast using Adobe Photoshop (Adobe). For three-dimensional sections, confocal images were acquired at 0.35–0.5-µm intervals.
Rabbit anti il 1β ab9722
Manufactured by Abcam
Rabbit anti-IL-1β (ab9722) is a primary antibody that recognizes the interleukin-1 beta (IL-1β) protein. IL-1β is a proinflammatory cytokine involved in the regulation of immune and inflammatory responses.
Automatically generated - may contain errors
Lab products found in correlation
Ab62698, by Abcam (1 mentions)
Alexa fluor conjugated phalloidin, by Thermo Fisher Scientific (1 mentions)
Cleaved il 1β 83186, by Cell Signaling Technology (1 mentions)
Vectashield mounting medium, by Vector Laboratories (1 mentions)
Rabbit anti map2, by Merck Group (1 mentions)
Fitc conjugated anti hrp, by Jackson ImmunoResearch (1 mentions)
Hrp conjugated anti mouse igg sc 2005, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti tdp 43 10782 2 ap, by Proteintech (1 mentions)
2 protocols using rabbit anti il 1β ab9722
1
Fluorescent Immunolabeling of Inflammatory Proteins
2
Antibody-based Protein Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following antibodies were used for immunoblotting: mouse anti-TurboGFP (TA150041) [Origene]; rabbit anti-TDP-43 (10782-2-AP) [Proteintech]; mouse anti-Lamin A/C (05-714) [EMD Millipore]; rabbit anti-beta Actin (ab16039) [Abcam]; rabbit anti-NF-κB p65 (Ser536) (3033), rabbit anti-NF-κB (8242) and HRP-conjugated anti-α-tubulin (9099) [Cell Signaling Technology]; and rabbit anti-PTP1B (sc-14021), HRP-conjugated anti-rabbit IgG (sc-2004) and HRP-conjugated anti-mouse IgG (sc-2005) [Santa Cruz]. The following antibodies were used for immunocytochemistry (ICC): rabbit anti-MAP2 (1:500; AB5622) and TRITC-conjugated phalloidin (1:500; 90228) [Millipore]. The following antibody was used for immunohistochemistry (IHC): FITC-conjugated anti-HRP (1:150; 123-095-021) [Jackson ImmunoResearch Laboratories]. The following antibodies were used for neutralizing target proteins: rabbit anti-IL-1β (ab9722) [Abcam]; rat anti-TNF-α (14-7321-81) [Invitrogen]; rabbit anti-IgG (2729) and mouse anti-IgG (5415) [Cell Signaling Technology]; and mouse anti-IL-6 (sc-57315) and rat anti-IgG (sc-2026) [Santa Cruz].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!