Anti mlc

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-MLC is a laboratory equipment product offered by Santa Cruz Biotechnology. It is designed to detect and quantify the presence of Myosin Light Chain (MLC) in biological samples. The core function of Anti-MLC is to provide researchers with a reliable tool for analyzing MLC levels, which is a key component in various cellular processes.

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3 protocols using anti mlc

Monitoring Phosphorylated Myosin Light Chain

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Anti-pMLC Ab specifically recognized the mono-phosphorylated MLC at Ser19 (Komatsu et al., 2000 (link)). Anti-MLC, RhoA, PKCα, PKGIα, ROCKα, PKGIβ, and ROCKβ antibodies (Abs) were purchased from Santa Cruz Biotechnology. Anti-smMHC Abs were purchased from abcam, MLCK from Pierce, MYPT1 (myosin phosphatase target subunit 1) from Millipore, and α-actin from Sigma.

Komatsu S., Kitazawa T, & Ikebe M. (2017). Visualization of stimulus-specific heterogeneous activation of individual vascular smooth muscle cells in aortic tissues. Journal of cellular physiology, 233(1), 434-446.

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Lipid-Induced Signaling Pathway Analysis

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Palmitate and oleate were obtained from Sigma-Aldrich. DMEM medium was obtained from Gibco. FBS was purchased from the Zhejiang Tianhang Biological Technology. anti-MLCK, anti-MLC, anti-MYPT1, anti-p-MYPT1, anti-ERK, anti-p-ERK and anti-β-actin antibodies were purchased from Santa Cruz Biotechnology. Anti-pMLC was obtained from Cell Signaling Technology. Anti-NOXA2/p67phox and anti-Rac family small GTPase 1 were purchased from Abcam.

Wang Y., Wang M., Yu P., Zuo L., Zhou Q., Zhou X, & Zhu H. (2020). MicroRNA-126 Modulates Palmitate-Induced Migration in HUVECs by Downregulating Myosin Light Chain Kinase via the ERK/MAPK Pathway. Frontiers in Bioengineering and Biotechnology, 8, 913.

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Melatonin Modulates Epithelial Barrier

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Melatonin was provided by School of Pharmacy, Anhui Medical University (Anhui, China). Dimethyl Sulfoxide (DMSO) was obtained from sigma Chemical (USA). Dulbeccos modified Eagles medium (DMEM) was purchased from Gibco BRL life Technologies (USA). Phorbol-12-myristate-13-acetate (PMA) and SP600125 were obtained from Cayman Chemical (USA). Primary antibodies (anti-OPN, anti-occludin, anti-MLCK, anti-pMLC, anti-MLC, anti-pJNK, anti-JNK, anti-β-actin) were purchased from Santa Cruz Biotechnology (USA). All secondary antibodies were purchased from Millipore (USA). ECL reagent and BCA kit were purchased from quantitative Pierce Company.

Zhou Q., Gui S., Zhou Q, & Wang Y. (2014). Melatonin Inhibits the Migration of Human Lung Adenocarcinoma A549 Cell Lines Involving JNK/MAPK Pathway. PLoS ONE, 9(7), e101132.

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