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Hrp conjugated goat f ab 2 anti mouse

Manufactured by Rockland Immunochemicals
Sourced in Japan

HRP)-conjugated goat F(ab')2 anti-mouse is a laboratory reagent designed for use in immunoassay techniques. It consists of goat-derived F(ab')2 antibody fragments that are conjugated to the enzyme horseradish peroxidase (HRP). This product can be used to detect and quantify mouse antibodies or antigens in various immunochemical applications.

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2 protocols using hrp conjugated goat f ab 2 anti mouse

1

Antibody Detection in Cell Samples

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The following commercial antibodies were used: Mouse monoclonal anti-vimentin (V9, Dako, Tokyo, Japan) and anti-β-actin (AC-15, Sigma, Tokyo, Japan); rabbit polyclonal anti-vimentin (SC-5565, Santa Cruz Biotechnology, Dallas, USA) and anti-GST (60–021, BioAcademia, Osaka, Japan); horseradish peroxidase (HRP)-conjugated goat F(ab')2 anti-mouse (710–1332, Rockland Immunochemicals, Limerick, USA) and goat anti-rabbit IgG (111–035-003, Jackson ImmunoResearch Laboratories, West Grove, USA).
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2

Immunoblotting and Immunofluorescence Assay

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The following commercial antibodies were used: mouse monoclonal anti-FLAG (M2, Sigma-Aldrich, St. Louis, MO, USA); rabbit polyclonal anti-FLAG (60–031, BioAcademia, Osaka, Japan) and anti-GFP (60–011, BioAcademia); HRP-conjugated goat F(ab’)2 anti-mouse (710–1332, Rockland Immunochemicals, Limerick, ME, USA) and goat anti-rabbit IgG (111–035–003, Jackson ImmunoResearch Laboratories, West Grove, PA, USA); Alexa 488-conjugated goat anti-rabbit IgG(H+L) (A-11034, ThermoFisher Scientific, Waltham, MA, USA) and Alexa 594-conjugated goat anti-mouse IgG(H+L) (A-11032, ThermoFisher Scientific).
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