The weight-average molecular weight Mw, number-average molecular weight Mn, and polydispersity of the sulfated GGM (SGGM) samples were determined by gel permeation chromatography (GPC) using an Agilent 1260 Infinity II Multi-Detector GPC/SEC system with two detectors: a refractometer (RI) and a viscometer (VS). The separation was made on two Agilent PL aquagel-OH columns. The samples were eluted with an eluent composed of 0.1 M NaNO3 and 0.25 g/L of NaN3 as a stabilizer (pH 7) at a flow rate 1 mL/min and a sample volume of 100 μL. Polyethylene glycol standards (Agilent, Santa Clara, CA, USA) were used to calibrate the columns. All the samples were dissolved overnight in the mobile phase (1–5 mg/mL) and then filtered through a 0.22-μm Agilent PES membrane filter to remove impurities. The data were collected and processed using the Agilent GPC/SEC MDS software.
Pes membrane filter
Manufactured by Agilent Technologies
The PES membrane filter is a laboratory filtration device made of polyethersulfone (PES) material. Its core function is to separate and retain particles, cells, or other suspended matter from liquid samples during the filtration process. The PES membrane provides a physical barrier for efficient removal of these components from the sample solution.
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2 protocols using pes membrane filter
1
Sulfated Galactoglucomannan: Molecular Weight Analysis
2
Globin Chain Separation by HPLC
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Globin chain separation was achieved by high-performance liquid chromatography (HPLC) using a LithoCART 250-4 column (VWR) and an Agilent 1260 infinity II HPLC system with acetonitrile-methanol gradients41 (link) Lysates were prepared from baboon peripheral blood by freeze-thaw lysis, clarified by centrifugation (12 000 rotation per minutes for 15 minutes), and filtered through a 22 μm Agilent PES membrane filter before injection.
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