Morpholinoethanesulfonic acid mes

Gelatin (Type B, from bovine skin, 225 g Bloom, average molecular weight=50,000, Cat# G9391), heparin (sodium salt from porcine intestinal mucosa, MW ∼ 17-19 kDa), and Type I collagenase from clostridium histolyticum were purchased from Sigma-Aldrich (St. Louis, MO, USA). 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and horseradish peroxidise (HRP, 304 units/mg) were purchased from Thermo Scientific (Rockford, IL, USA). Morpholinoethanesulfonic acid (MES) and N-hydroxysulfosuccinimide (NHS) were purchased from Acros Organics (New Jersey, USA). Hydrogen peroxide (H₂O₂) aqueous solution (35%, wt/wt) was purchased from BDH Chemicals (Westchester, PA, USA). Dialysis membrane (MWCO: 10 kD and 50 kD) were purchased from Spectrum® Laboratories (Dallas, TX, USA). Human umbilical vein endothelial cells (HUVECs) and endothelial cell growth media kits (EGM-2 BulletKit) were purchased from the Lonza Company (Allendale, NJ, USA). Recombinant mouse VEGF₁₆₄ and mouse VEGF Quantikine ELISA Kits were purchased from R&D Systems, Inc (Minneapolis, MN, USA). Fertile eggs (White Leghorns) were purchased from the Texas A&M University poultry science department (Bryan, TX, USA).

Gelatin (type B, from bovine skin, 225 g Bloom), heparin (sodium salt from porcine intestinal mucosa, MW ≈ 17–19 kDa), bovine serum albumin (BSA), fluorescein isothiocyanate (FITC), and type I collagenase from Clostridium histolyticum were purchased from Sigma-Aldrich (St Louis, MO). 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) was purchased from Thermo Scientific (Rockford, IL). Morpholinoethanesulfonic acid (MES) and N-hydroxysuccinimide (NHS) were purchased from Acros Organics (New Jersey). Dialysis membrane (molecular weight cutoff (MW cutoff 50 kDa)) was purchased from Spectrum Laboratories (Dallas, TX). Mineral oil, isopropanol, 1,4-dioxane, ethanol, and glycine were purchased from VWR.