Bradford protein assay reagent

Enzyme-linked immunosorbent assay (ELISA) plates were purchased from AGC (Tokyo, Japan). Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgE antibody was purchased from Southern Biotech (Birmingham, AL, USA). HRP-conjugated goat anti-mouse IgG1 antibody was purchased from Bethyl Laboratories (Montgomery, TX, USA). 3,3,5,5-Tetramethylbenzidine (TMB) peroxidase substrate was purchased from Kirkegaard & Perry Laboratories (KPL; Gaithersburg, MD, USA). Coomassie brilliant blue (CBB) R-250, Bradford protein assay reagent, and pentobarbital sodium salt were purchased from Nacalai Tesque (Kyoto, Japan). Enhanced chemiluminescence (ECL) western blotting substrates and X-ray films (Amersham Hyperfilm MP) were purchased from GE Healthcare (Chalfont St. Giles, UK). Luminata Crescendo Western HRP Substrate was purchased from Merck Millipore (Burlington, MA, USA). Immunoreactive enhancers, Can Get Signal solutions 1 and 2, were purchased from TOYOBO (Osaka, Japan). All other chemicals used in this study were of the highest purity available.

Enzyme-linked immunosorbent assay (ELISA) plates were purchased from AGC (Tokyo, Japan). Horseradish-peroxidase (HRP)-conjugated goat antimouse IgE antibody was purchased from Southern Biotech (Birmingham, AL, USA). HRP-conjugated goat antimouse IgG1-antibody was purchased from Bethyl Laboratories (Montgomery, TX, USA). 3,3′,5,5′-tetramethylbenzidine (TMB) peroxidase substrate was purchased from Kirkegaard & Perry Laboratories (Gaithersburg, MD, USA). Coomassie Brilliant Blue R-250, Bradford protein assay reagent, and pentobarbital sodium salt were purchased from Nacalai Tesque (Kyoto, Japan). Enhanced chemiluminescence (ECL) Western blotting substrate and X-ray films (Amersham Hyperfilm MP) were purchased from GE Healthcare (Chalfont St. Giles, UK). Immunoreactive enhancers, CanGet Signal solutions 1 and 2, were purchased from TOYOBO (Osaka, Japan). All other chemicals used in this study were of the highest purity available.