Cd3 fluorescein isothiocyanate

Manufactured by Thermo Fisher Scientific

CD3-fluorescein isothiocyanate is a fluorescent-labeled antibody that binds to the CD3 antigen, a protein complex expressed on the surface of T cells. It is used in flow cytometry and other immunological applications to identify and quantify T cell populations.

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Anti-CD3 fluorescein isothiocyanate (FITC)-conjugated antibody (3 citations) CD3 fluorescein isothiocyanate (FITC), (2 citations) Fluorescein isothiocyanate (FITC)-conjugated CD3 (2 citations)

2 protocols using cd3 fluorescein isothiocyanate

Multiparametric Immune Cell Analysis

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All antibodies were purchased from commercial sources: CD3-phycoerythrin (PE), CD4-allophycocyanin (APC), CD4-peridinin-chlorophyll–protein complex (PerCP), CD19-PE, CD3-APC, CD3-fluorescein isothiocyanate (FITC), CD8-PE, NK1.1-APC, F4/80-PE, CD11c-PE, CD11c-APC, IL-4-PE, IFN-γ-APC, TNFα-FITC, CD69-APC, anti-CD3 Abs, and anti-CD28 Abs from eBioscience; Rabbit polyclonal GIT2 Abs, anti-phospho-PAK1/2 (Ser199/204 of PAK1 and Ser192/197 of PAK2 Abs, and anti-phospho-c-Cbl (Y774) Abs from Cell Signaling; Anti-phospho-Erk1/2 (E4) Abs, anti-PAKα, anti-Erk1/2 (K23) from Santa Cruz.

Hao Y.E., He D.F., Yin R.H., Chen H., Wang J., Wang S.X., Zhan Y.Q., Ge C.H., Li C.Y., Yu M, & Yang X.M. (2015). GIT2 deficiency attenuates concanavalin A-induced hepatitis in mice. FEBS Open Bio, 5, 688-704.

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T-Cell Surface Phenotyping by Flow Cytometry

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The surface phenotype of the T-cell clone was determined by staining with CD3-fluorescein isothiocyanate, CD8-phycoethythrin, CD4-PerCP-Cyanine5.5, and CD56 (NCAM)-allophycocyanin (eBioscience, San Diego, CA). Isotype-matched antibodies were used as a negative control. Data were acquired and using FACS Fortessa and analyzed using FACS Diva (BD Biosciences, San Jose, CA) at the University of Arkansas for Medical Sciences Microbiology and Immunology Flow Cytometry Core Laboratory.

Coleman H.N., Wang X., Greenfield W.W, & Nakagawa M. (2014). A Human Papillomavirus Type 16 E6 52-62 CD4 T-Cell Epitope Restricted by the HLA-DR11 Molecule Described in an Epitope Hotspot. MOJ immunology, 1(3), 00018.

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