PET scans were carried out with Eminence SET-3000GCT-X (Shimadzu Corp) to measure regional brain radioactivity. This scanner provides 99 sections with an axial field of view of 26.0 cm. Spatial resolution was 3.45 mm in-plane and 3.72 mm axially full-width at half-maximum. A head fixation device was used during the scans. A 15-min transmission scan was done to correct for attenuation using a 137Cs source. Dynamic PET scan was performed for 90 min (1 min×15, 5 min×15) after i.v. bolus injection of [11C]-(+)-PHNO. Injected radioactivity was 196.4 to 385.0 MBq (348.8±64.2 (mean±SD MBq) for drug-free condition; 339.8±70.2 MBq for blonanserin condition). The injected mass of [11C]-(+)-PHNO was 1.9 – 2.5 μg (2.2±0.3 μg for drug-free condition; 2.4±0.1 μg for blonanserin condition). Specific radioactivity was 41.7 – 95.4 GBq/µmol (77.7±7.5 GBq/µmol for drug-free condition; 77.9±7.5 GBq/µmol for blonanserin condition) at the time of injection.
Eminence set 3000gct x
Manufactured by Shimadzu
Sourced in Japan
The Eminence SET-3000GCT-X is a gas chromatograph-triple quadrupole mass spectrometer (GC-MS/MS) system manufactured by Shimadzu. It is designed for the analysis of complex samples, providing high-performance separation and detection capabilities. The core function of this instrument is to perform sensitive and selective quantitative and qualitative analysis of organic compounds in various matrices.
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6 protocols using eminence set 3000gct x
1
Quantifying D2/D3 Receptor Availability via [11C]-(+)-PHNO PET
2
PET Quantification of [11C]-(+)-PHNO Binding
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PET scans were performed with Eminence SET-3000GCT-X (Shimadzu Corp, Kyoto, Japan) to measure regional brain radioactivity. This scanner provides 99 sections with an axial field of view of 26.0 cm. Spatial resolution was 3.45 mm in-plane and 3.72 mm axially full-width at half-maximum. A head fixation device was used during the scans. A 15-min transmission scan was done to correct for attenuation using a 137Cs source. Dynamic PET scan was performed for 90 min (1 min × 15, 5 min × 15) after i.v. bolus injection of [11C]-(+)-PHNO. Injected radioactivity was 139.1 to 386.4 MBq (309.8 ± 79.7 (mean ± SD MBq) for olanzapine-condition; 351.2 ± 34.3 MBq for blonanserin-condition). The injected mass of [11C]-(+)-PHNO was 0.5–2.5 μg (2.0 ± 0.7 μg for olanzapine-condition; 2.4 ± 0.3 μg for blonanserin-condition). Molar radioactivity was 55.3–141.0 GBq/μmol (86.0 ± 25.7 GBq/μmol for olanzapine-condition; 77.5 ± 21.9 GBq/μmol for blonanserin -condition) at the time of injection.
3
PET Imaging of Dopamine D2 Receptors with [11C]raclopride
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An Eminence SET-3000GCT/X (Shimadzu Corporation, Kyoto, Japan) PET scanner was used to measure radioactivity in the striatum. Dynamic PET scanning was performed for 30 to 60 minutes after i.v. bolus injection of 211.1 to 234.6 MBq/1.04 [0.44] (mean [SD]) μg [11C]raclopride. The specific radioactivity of [11C]raclopride was 33.5 to 163.3 GBq/μmol, and the injected mass of [11C]raclopride was 0.47 to 2.35 μg. MRI of the brain was acquired with 1.5T MR imaging, Intera 1.5T Achieva Nova (Philips Medical Systems, Best, the Netherlands) as proton density image (echo time = 17 milliseconds; repetition time = 6000 milliseconds; field of view = 22 cm, 2-dimensional, 256 × 256; slice thickness = 2 mm; number of excitations = 2). These images were used for analysis of the PET scans.
4
PET Imaging of Dopamine Receptors
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After intravenous injection of [11C]raclopride (228.4 ± 8.1 MBq with specific activity of 158.4 ± 53.5 GBq/µmol), three-dimensional dynamic images were acquired on a PET camera (Eminence SET-3000GCT/X, Shimadzu, Kyoto, Japan) for 60 min. All PET images were reconstructed by filtered back-projection method (Gaussian filter, kernel 5 mm; the reconstructed in-plane resolution was 7.5 mm in full-width at half-maximum; voxel size: 2 × 2 × 2.6 mm) corrected for attenuation, randoms, scatter and head motion.
5
Brain Imaging with [18F]FE-PE2I PET
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An Eminence SET-3000GCT-X (Shimadzu Corp., Japan) scanner system was used for all measurements, with a head fixation device to minimize head movement. A 10-min transmission scan was performed to correct for attenuation. Dynamic PET scan was performed for 60 min after intravenous bolus injection of [18F]FE-PE2I. Injected radioactivity was 182.9-191.1 (mean ± S.D = 188.2 ± 2.3) MBq at baseline condition, 180.5–189.7 (186.0 ± 2.6) MBq for placebo, and 182.3–190.8 (188.1 ± 2.4) MBq for dl-methylephedrine. Specific radioactivity was 566.8–728.0 (641.7 ± 77.4) GBq/μmol at baseline condition, 581.5–1072.7 (960.4±171.6) GBq/μmol for placebo and 544.0–1072.73 (894.2 ± 313.6) GBq/μmol for dl-methylephedrine.
6
PET Imaging of 5-HT1A Receptor and Extracellular 5-HT
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We used 11C-WAY-100635 (228.3 ± 10.8 MBq, molar activity (Am): 147.8 ± 100 GBq/μmol) for assessing 5-HT1A receptor density. We also employed 18F-MPPF (186.4 ± 96 MBq, Am: 174.8 ± 6.5 GBq/μmol) for evaluating extracellular 5-HT concentration in addition to receptor density. Out of 27 participants, 25 individuals received PET scans first with 18F-MPPF and then with 11C-WAY-100635, whereas 1 healthy control and 1 patient with depression underwent 2 PET scans in reverse order. The mean interval between the 2 PET sessions was 18 days, ranging from 7 to 68 days. After i.v. injection, 3-dimensional dynamic images were acquired using a PET scanner (Eminence SET-3000GCT/X; Shimadzu, Kyoto, Japan) for 90 and 60 minutes for 11C-WAY-100635 and 18F-MPPF, respectively. All PET images were reconstructed using the filtered back-projection method (Gaussian filter, kernel 5 mm; reconstructed in-plane resolution, 7.5 mm full width at half maximum; voxel size, 2 × 2 × 2.6 mm) with correction for attenuation, randoms, and scatter. Head motion during the scans was corrected on the emission images after attenuation correction with μ-maps realigned to each frame of the emission images (Wardak et al., 2010 (link)).
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