Csu xm2

Manufactured by Nikon

The CSU-Xm2 is a compact and reliable confocal scanning unit designed for use in advanced microscopy applications. It provides high-quality optical performance and enables precise control of the confocal aperture to optimize image quality and resolution. The CSU-Xm2 is a core component of Nikon's high-end microscopy systems, offering researchers a versatile tool for a wide range of imaging tasks.

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Lab products found in correlation

Te2000 u, by Nikon (5 mentions) Ixon3 897, by Oxford Instruments (2 mentions) Na 1.4 oil objective, by Nikon (2 mentions) Lsm 880, by Zeiss (2 mentions)

5 protocols using csu xm2

Live-cell and super-resolution imaging

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Imaging was performed with an inverted fluorescence microscope (TE2000-U; Nikon) equipped with an electron-multiplying charge-coupled device camera (Cascade II; Photometrics) and a Yokogawa spinning disc confocal system (CSU-Xm2; Nikon). Live-cell imaging was performed at 37°C. Images were captured with a 100x NA 1.4 oil objective and acquired using MetaMorph (version 7.0; MDS Analytical Technologies).
Super-resolution microscopy (SIM) was performed with an inverted fluorescence microscope (TI-E; Nikon) equipped with an electron-multiplying charge-coupled device camera (iXon3 897; Andor). Z-stack images were captured every 200 nm and reconstructed using NIS elements with SIM (Nikon).

Lee J.E., Westrate L.M., Wu H., Page C, & Voeltz G.K. (2016). Multiple Dynamin family members collaborate to drive mitochondrial division. Nature, 540(7631), 139-143.

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Imaging of Fixed and Live Cells

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Fixed cells were imaged at room temperature. Live cells were kept at 37°C in a live-cell incubation chamber (Pathology Devices, Westminster, MD). Cell imaging was performed with an inverted fluorescence microscope (TE2000-U; Nikon, Melville, NY) equipped with a Yokogawa spinning-disk confocal system (CSU-Xm2; Nikon). Images were taken with a 100× NA 1.4 oil objective or 60X NA 1.4 oil objective (Nikon) on electron-multiplying charge-coupled device (CCD) camera 50X50 (Cascade II; Photometrics), 50×50 (Andor) or 1TB (Andor). Images were acquired with MetaMorph 7.0 (MDS Analytical Technologies, Sunnyvale, CA), with Nikon Elements, or with Micromanager and then analyzed, merged, and contrasted using Fiji (ImageJ), as well as converted to 400dpi using Photoshop (Adobe, San Jose, CA). Scale bars were generated using Fiji. Supplemental videos were generated using ImageJ, Adobe Photoshop, and Quicktime.

Hoyer M.J., Chitwood P.J., Ebmeier C.C., Striepen J.F., Qi R.Z., Old W.M, & Voeltz G.K. (2018). A novel class of ER membrane proteins regulates ER-associated endosome fission. Cell, 175(1), 254-265.e14.

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Live-cell and super-resolution imaging

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Lee J.E., Westrate L.M., Wu H., Page C, & Voeltz G.K. (2016). Multiple Dynamin family members collaborate to drive mitochondrial division. Nature, 540(7631), 139-143.

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Live-Cell Imaging of ER Contact

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Imaging was performed with an inverted fluorescence microscope (TE2000-U; Nikon) equipped with an electron-multiplying charge-coupled device camera (Cascade II; Photometrics) and a Yokogawa spinning disc confocal system (CSU-Xm2; Nikon). Live-cell imaging was performed at 37°C. Images were captured with a 100× NA 1.4 oil objective and acquired using the open source microscopy software Micro-Manager. Live-cell super-resolution capture of ddFP-marked ER contact during PB fission was acquired with the Zeiss LSM 880 equipped with Airyscan detectors and 63×/1.4-NA plan Apochromat oil objective using Zeiss ZEN software.

Lee J.E., Cathey P.I., Wu H., Parker R, & Voeltz G.K. (2020). Endoplasmic reticulum contact sites regulate the dynamics of membraneless organelles. Science (New York, N.Y.), 367(6477), eaay7108.

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Live-Cell Imaging with Spinning-Disk Confocal Microscopy

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Live-cell imaging was performed with an inverted fluorescence microscope (TE2000-U; Nikon, Melville, NY) equipped with an electron-multiplying charge-coupled device (CCD) camera (Cascade II; Photometrics) and a Yokogawa spinning-disk confocal system (CSU-Xm2; Nikon). Images were taken with a 100× numerical aperture 1.4 oil objective (Nikon). While imaging, live cells were kept at 37°C in a live-cell incubation chamber (Pathology Devices, Westminster, MD). Images were acquired with MetaMorph 7.0 (MDS Analytical Technologies, Sunnyvale, CA), analyzed, merged, and contrasted using ImageJ, as well as contrasted and converted to 400dpi using Photoshop (Adobe, San Jose, CA). Scale bars were generated using ImageJ. Supplemental videos were generated using ImageJ.

Rowland A.A., Chitwood P.J., Phillips M.J, & Voeltz G.K. (2014). ER contact sites define the position and timing of endosome fission. Cell, 159(5), 1027-1041.

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